methanogenium_sp_medium (CultureMech:005910)

Overview

External ontology

komodo.medium:532 (source link unavailable) — METHANOGENIUM SP. medium

Applications

Microbial cultivation

Notes

KOMODO MediaInfo 532 lists 27 quantified g/L components for METHANOGENIUM SP. medium and also lists CO2, H2O, H2, distilled water, and NaOH without gram or mol amounts.

Composition

flowchart LR
    CultureMech_005910["methanogenium_sp_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_005910 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_005910 --> ing1_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing1_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing2_HCl["HCl"]
    CultureMech_005910 --> ing2_HCl
    CHEBI_17883["CHEBI:17883"]
    ing2_HCl -.-> CHEBI_17883
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_005910 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_005910 --> ing4_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing4_Na2S_x_9_H2O -.-> CHEBI_76209
    ing5_ZnCl2["ZnCl2"]
    CultureMech_005910 --> ing5_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing5_ZnCl2 -.-> CHEBI_49976
    ing6_2_Methylbutyric_acid["2-Methylbutyric acid"]
    CultureMech_005910 --> ing6_2_Methylbutyric_acid
    CHEBI_37070["CHEBI:37070"]
    ing6_2_Methylbutyric_acid -.-> CHEBI_37070
    ing7_NH4Cl["NH4Cl"]
    CultureMech_005910 --> ing7_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing7_NH4Cl -.-> CHEBI_31206
    ing8_CO2["CO2"]
    CultureMech_005910 --> ing8_CO2
    CHEBI_16526["CHEBI:16526"]
    ing8_CO2 -.-> CHEBI_16526
    ing9_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_005910 --> ing9_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing9_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing10_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_005910 --> ing10_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing10_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing11_H3BO3["H3BO3"]
    CultureMech_005910 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_005910 --> ing12_NiCl2_x_6_H2O
    CHEBI_53542["CHEBI:53542"]
    ing12_NiCl2_x_6_H2O -.-> CHEBI_53542
    ing13_Isovaleric_acid["Isovaleric acid"]
    CultureMech_005910 --> ing13_Isovaleric_acid
    CHEBI_28484["CHEBI:28484"]
    ing13_Isovaleric_acid -.-> CHEBI_28484
    ing14_Isobutyric_acid["Isobutyric acid"]
    CultureMech_005910 --> ing14_Isobutyric_acid
    CHEBI_16135["CHEBI:16135"]
    ing14_Isobutyric_acid -.-> CHEBI_16135
    ing15_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_005910 --> ing15_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing15_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing16_H2O["H2O"]
    CultureMech_005910 --> ing16_H2O
    CHEBI_15377["CHEBI:15377"]
    ing16_H2O -.-> CHEBI_15377
    ing17_Cysteine_HCl_x_H2O["Cysteine-HCl x H2O"]
    CultureMech_005910 --> ing17_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing17_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing18_Resazurin["Resazurin"]
    CultureMech_005910 --> ing18_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing18_Resazurin -.-> CHEBI_8806
    ing19_Na_formate["Na-formate"]
    CultureMech_005910 --> ing19_Na_formate
    CHEBI_62965["CHEBI:62965"]
    ing19_Na_formate -.-> CHEBI_62965
    ing20_NaHCO3["NaHCO3"]
    CultureMech_005910 --> ing20_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing20_NaHCO3 -.-> CHEBI_32139
    ing21_Yeast_extract["Yeast extract"]
    CultureMech_005910 --> ing21_Yeast_extract
    ing22_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_005910 --> ing22_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing22_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing23_Valeric_acid["Valeric acid"]
    CultureMech_005910 --> ing23_Valeric_acid
    CHEBI_17418["CHEBI:17418"]
    ing23_Valeric_acid -.-> CHEBI_17418
    ing24_Na_acetate["Na-acetate"]
    CultureMech_005910 --> ing24_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing24_Na_acetate -.-> CHEBI_32954
    ing25_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_005910 --> ing25_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing25_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing26_H2["H2"]
    CultureMech_005910 --> ing26_H2
    CHEBI_18276["CHEBI:18276"]
    ing26_H2 -.-> CHEBI_18276
    ing27_Distilled_water["Distilled water"]
    CultureMech_005910 --> ing27_Distilled_water
    ing27_Distilled_water -.-> CHEBI_15377
    ing28_NaCl["NaCl"]
    CultureMech_005910 --> ing28_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing28_NaCl -.-> CHEBI_26710
    ing29_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_005910 --> ing29_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing29_FeCl2_x_4_H2O -.-> CHEBI_86249
    CultureMech_005910 --> more["...2 more"]

Ingredients (32)

#	Ingredient	Concentration	Ontology	Notes
1	KH2PO4 H2O4P.K 136.084 g/mol	0.49 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	KOMODO MediaInfo 532 lists 0.49 g/L KH2PO4.
2	CoCl2 x 6 H2O 2Cl.Co.6H2O 237.929 g/mol	1.87E-4 G_PER_L	CHEBI:53503 cobalt chloride hexahydrate	KOMODO MediaInfo 532 lists 1.87E-4 g/L CoCl2 x 6 H2O.
3	HCl HCl 36.461 g/mol	6.65E-4 G_PER_L	CHEBI:17883 hydrogen chloride	KOMODO MediaInfo 532 lists 6.65E-4 g/L HCl.
4	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.05 G_PER_L	CHEBI:86158 calcium chloride dihydrate	KOMODO MediaInfo 532 lists 0.05 g/L CaCl2 x 2 H2O.
5	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.49 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	KOMODO MediaInfo 532 lists 0.49 g/L Na2S x 9 H2O.
6	ZnCl2 2Cl.Zn 136.296 g/mol	6.91E-5 G_PER_L	CHEBI:49976 zinc dichloride	KOMODO MediaInfo 532 lists 6.91E-5 g/L ZnCl2.
7	2-Methylbutyric acid C5H10O2 102.133 g/mol	0.49 G_PER_L	CHEBI:37070 2-methylbutyric acid	KOMODO MediaInfo 532 lists 0.49 g/L 2-Methylbutyric acid.
8	NH4Cl Cl.H4N 53.492 g/mol	0.40 G_PER_L	CHEBI:31206 ammonium chloride	KOMODO MediaInfo 532 lists 0.40 g/L NH4Cl.
9	CO2 CO2 44.009 g/mol		CHEBI:16526 carbon dioxide	KOMODO MediaInfo 532 lists CO2 without a gram or mol amount.
10	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	1.97E-6 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	KOMODO MediaInfo 532 lists 1.97E-6 g/L CuCl2 x 2 H2O.
11	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	3.55E-5 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	KOMODO MediaInfo 532 lists 3.55E-5 g/L Na2MoO4 x 2 H2O.
12	H3BO3 H3BO3 61.833 g/mol	5.92E-6 G_PER_L	CHEBI:33118 boric acid	KOMODO MediaInfo 532 lists 5.92E-6 g/L H3BO3.
13	NiCl2 x 6 H2O 2Cl.6H2O.Ni 237.689 g/mol	2.52E-3 G_PER_L	CHEBI:53542 nickel chloride hexahydrate	KOMODO MediaInfo 532 lists 2.52E-3 g/L NiCl2 x 6 H2O.
14	Isovaleric acid C5H10O2 102.133 g/mol	0.49 G_PER_L	CHEBI:28484 isovaleric acid	KOMODO MediaInfo 532 lists 0.49 g/L Isovaleric acid.
15	Isobutyric acid C4H8O2 88.106 g/mol	0.49 G_PER_L	CHEBI:16135 isobutyric acid	KOMODO MediaInfo 532 lists 0.49 g/L Isobutyric acid.
16	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.40 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	KOMODO MediaInfo 532 lists 0.40 g/L MgSO4 x 7 H2O.
17	H2O H2O 18.015 g/mol		CHEBI:15377 water	KOMODO MediaInfo 532 lists H2O without a gram or mol amount.
18	Cysteine-HCl x H2O C3H7NO2S.H2O.HCl 175.637 g/mol	0.49 G_PER_L	CHEBI:91248 L-cysteine hydrochloride hydrate	KOMODO MediaInfo 532 lists 0.49 g/L Cysteine-HCl x H2O.
19	Resazurin C12H7NO4 229.191 g/mol	9.89E-4 G_PER_L	CHEBI:8806 Resazurin	KOMODO MediaInfo 532 lists 9.89E-4 g/L Resazurin.
20	Na-formate CHO2.Na 68.007 g/mol	1.98 G_PER_L	CHEBI:62965 sodium formate	KOMODO MediaInfo 532 lists 1.98 g/L Na-formate.
21	NaHCO3 CHO3.Na 84.006 g/mol	3.96 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	KOMODO MediaInfo 532 lists 3.96 g/L NaHCO3.
22	Yeast extract	1.19 G_PER_L	FOODON:03315426 yeast extract	KOMODO MediaInfo 532 lists 1.19 g/L Yeast extract.
23	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	9.87E-5 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	KOMODO MediaInfo 532 lists 9.87E-5 g/L MnCl2 x 4 H2O.
24	Valeric acid C5H10O2 102.133 g/mol	0.49 G_PER_L	CHEBI:17418 valeric acid	KOMODO MediaInfo 532 lists 0.49 g/L Valeric acid.
25	Na-acetate C2H3O2.Na 82.034 g/mol	0.99 G_PER_L	CHEBI:32954 sodium acetate	KOMODO MediaInfo 532 lists 0.99 g/L Na-acetate.
26	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	1.98E-3 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate	KOMODO MediaInfo 532 lists 1.98E-3 g/L FeSO4 x 7 H2O.
27	H2 H2 2.016 g/mol		CHEBI:18276 dihydrogen	KOMODO MediaInfo 532 lists H2 without a gram or mol amount.
28	Distilled water H2O 18.015 g/mol		CHEBI:15377 water	KOMODO MediaInfo 532 lists Distilled water without a gram or mol amount.
29	NaCl Cl.Na 58.443 g/mol	5.40 G_PER_L	CHEBI:26710 sodium chloride	KOMODO MediaInfo 532 lists 5.40 g/L NaCl.
30	FeCl2 x 4 H2O Cl2Fe.4H2O 198.811 g/mol	1.48E-3 G_PER_L	CHEBI:86249 iron dichloride tetrahydrate	KOMODO MediaInfo 532 lists 1.48E-3 g/L FeCl2 x 4 H2O.
31	NaOH HO.Na 39.997 g/mol		CHEBI:32145 sodium hydroxide	KOMODO MediaInfo 532 lists NaOH without a gram or mol amount.
32	sludge from an anaerobic digester	49.46 G_PER_L		KOMODO MediaInfo 532 lists 49.46 g/L sludge from an anaerobic digester.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:03.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 532
2026-03-13T06:19:37.204494Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:005910
2026-03-17T19:45:53.922259+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "METHANOGENIUM SP. medium" to "methanogenium_sp_medium" for consistent matching
2026-08-06T08:58:32.366253+00:00	curate_flag_empty_composition.py	FLAGGED_INCOMPLETE_COMPOSITION	Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175).
2026-09-10T00:00:00-07:00	repair_komodo_532_score35.py	RESOLVED_KOMODO_532_SCORE35	KOMODO MediaInfo 532 lists 27 quantified g/L components for METHANOGENIUM SP. medium and also lists CO2, H2O, H2, distilled water, and NaOH without gram or mol amounts.