Overview
- External ontology
- komodo.medium:545a (source link unavailable) — DILUTED TRYPTONE SOYA BROTH (TSB)
- Applications
-
- Microbial cultivation
- pH
- 8.0
- Notes
- Source: KOMODO ModelSEED | ID: 545a | DSMZ Medium: 545a (mediadive.medium:545a) | Aerobic: No
Composition
flowchart LR
CultureMech_005986["diluted_tryptone_soya_broth_tsb"]
ing0_Casein_peptone["Casein peptone"]
CultureMech_005986 --> ing0_Casein_peptone
ing1_Soy_peptone["Soy peptone"]
CultureMech_005986 --> ing1_Soy_peptone
ing2_D____Glucose["D(+)-Glucose"]
CultureMech_005986 --> ing2_D____Glucose
CHEBI_17634["CHEBI:17634"]
ing2_D____Glucose -.-> CHEBI_17634
ing3_NaCl["NaCl"]
CultureMech_005986 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_K2HPO4["K2HPO4"]
CultureMech_005986 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_Sodium_resazurin["Sodium resazurin"]
CultureMech_005986 --> ing5_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Sodium_resazurin -.-> CHEBI_8806
ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_005986 --> ing6_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing7_Na_thioglycolate["Na-thioglycolate"]
CultureMech_005986 --> ing7_Na_thioglycolate
CHEBI_86481["CHEBI:86481"]
ing7_Na_thioglycolate -.-> CHEBI_86481
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casein peptone | 17 G_PER_L | ||
| 2 | Soy peptone | 3 G_PER_L | ||
| 3 |
D(+)-Glucose
C6H12O6 180.156 g/mol
|
2.5 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 7 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.3 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 8 |
Na-thioglycolate
C2H3O2S.Na 114.101 g/mol
|
0.3 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 545a |
| 2026-02-03T11:19:18.780655 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 545 |
| 2026-03-13T06:19:37.470422Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005986 |
| 2026-03-17T06:30:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:54.246702+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DILUTED TRYPTONE SOYA BROTH (TSB)" to "diluted_tryptone_soya_broth_tsb" for consistent matching |
| 2026-06-05T07:40:24.541829+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:45.275359+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |