medium_547_modified_for_dsm_44048 (CultureMech:005988)

Overview

External ontology

komodo.medium:547_44048 (source link unavailable) — MEDIUM 547 MODIFIED FOR DSM 44048

Applications

Microbial cultivation

pH

7.2

Notes

Source: KOMODO ModelSEED | ID: 547_44048 | DSMZ Medium: 547 (mediadive.medium:547) | Aerobic: No

Composition

flowchart LR
    CultureMech_005988["medium_547_modified_for_dsm_44048"]
    ing0_Agar["Agar"]
    CultureMech_005988 --> ing0_Agar
    CHEBI_2509["CHEBI:2509"]
    ing0_Agar -.-> CHEBI_2509
    ing1_Starch["Starch"]
    CultureMech_005988 --> ing1_Starch
    CHEBI_28017["CHEBI:28017"]
    ing1_Starch -.-> CHEBI_28017
    ing2_CaCO3["CaCO3"]
    CultureMech_005988 --> ing2_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing2_CaCO3 -.-> CHEBI_3311
    ing3_K2HPO4["K2HPO4"]
    CultureMech_005988 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_005988 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_NaCl["NaCl"]
    CultureMech_005988 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6__NH4_2SO4["(NH4)2SO4"]
    CultureMech_005988 --> ing6__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing6__NH4_2SO4 -.-> CHEBI_62946

Ingredients (7)

#	Ingredient	Concentration	Ontology
1	Agar	19.98 G_PER_L	CHEBI:2509 agar
2	Starch	20 G_PER_L	CHEBI:28017 starch
3	CaCO3 CO3.Ca 100.086 g/mol	3.99202 G_PER_L	CHEBI:3311 calcium carbonate
4	K2HPO4 HO4P.2K 174.174 g/mol	1.99601 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate
5	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	1.99601 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
6	NaCl Cl.Na 58.443 g/mol	1.99601 G_PER_L	CHEBI:26710 sodium chloride
7	(NH4)2SO4 2H4N.O4S 132.141 g/mol	3.99202 G_PER_L	CHEBI:62946 ammonium sulfate

Solutions

Trace salt solution

Dose: 1 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
FeSO4 x 7 H2O	1 G_PER_L
MnCl2 x 4 H2O	1 G_PER_L
ZnSO4 x 7 H2O	1 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:03.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 547_44048
2026-02-03T11:19:03.482968	dsmz-resolver-v1.0	Enriched with DSMZ composition	Copied ingredients from DSMZ Medium 547
2026-03-13T06:19:37.479221Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:005988
2026-03-17T06:30:06.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:54.257750+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "MEDIUM 547 MODIFIED FOR DSM 44048" to "medium_547_modified_for_dsm_44048" for consistent matching
2026-06-05T07:41:25.531676+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:04.943492+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-13T07:04:22.114827+00:00	apply_cocktail_nesting.py	NESTED_FLATTENED_COCKTAIL	Moved 3 stock-strength component(s) out of ingredients into 1 solution(s): 3 -> 'Trace salt solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).