Overview
- External ontology
- komodo.medium:549 (source link unavailable) — CM + YE medium
- Applications
-
- Microbial cultivation
- pH
- 7.4
- High-metal flag
- True
- Notes
- pH buffer: NaOH | Source: KOMODO ModelSEED | ID: 549 | DSMZ Medium: 549 (mediadive.medium:549) | Aerobic: No
Composition
flowchart LR
CultureMech_005996["cm_ye_medium"]
ing0_Casamino_acids["Casamino acids"]
CultureMech_005996 --> ing0_Casamino_acids
ing1_Yeast_extract["Yeast extract"]
CultureMech_005996 --> ing1_Yeast_extract
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_005996 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_Na3_citrate["Na3-citrate"]
CultureMech_005996 --> ing3_Na3_citrate
CHEBI_53258["CHEBI:53258"]
ing3_Na3_citrate -.-> CHEBI_53258
ing4_KCl["KCl"]
CultureMech_005996 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_NaCl["NaCl"]
CultureMech_005996 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_Agar["Agar"]
CultureMech_005996 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_005996 --> ing7_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
ing8_HCl["HCl"]
CultureMech_005996 --> ing8_HCl
CHEBI_17883["CHEBI:17883"]
ing8_HCl -.-> CHEBI_17883
ing9_NaOH["NaOH"]
CultureMech_005996 --> ing9_NaOH
CHEBI_32145["CHEBI:32145"]
ing9_NaOH -.-> CHEBI_32145
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casamino acids | 7.5 G_PER_L | ||
| 2 | Yeast extract | 10 G_PER_L | ||
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
20 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
Na3-citrate
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
200 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 8 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
10 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 9 |
HCl
HCl 36.461 g/mol
|
0.03646 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 10 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 549 |
| 2026-02-03T11:19:05.275305 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 549 |
| 2026-03-13T03:09:05.567297Z | data-quality-cleanup-v1.0 | Data quality fixes | Added pH buffer: NaOH |
| 2026-03-13T06:19:37.517788Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:005996 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-17T06:30:11.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:54.306840+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CM + YE medium" to "cm_ye_medium" for consistent matching |
| 2026-05-16T09:03:03.446524+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:24.568219+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:28.343504+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |