glutarate_medium (CultureMech:006014)

Overview

External ontology

komodo.medium:562 (source link unavailable) — GLUTARATE medium

Applications

Microbial cultivation

pH

7.2

Notes

Archived DSMZ Medium 562 defines GLUTARATE MEDIUM as DSMZ Medium 298 with butanediol replaced by 2.6 g/L sodium glutarate and with 2% v/v rumen fluid; this record expands archived DSMZ Media 298, 320, and 562 into final per-liter components.

Composition

flowchart LR
    CultureMech_006014["glutarate_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_006014 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_006014 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_NaCl["NaCl"]
    CultureMech_006014 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_006014 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_KCl["KCl"]
    CultureMech_006014 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_006014 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_Resazurin["Resazurin"]
    CultureMech_006014 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_HCl["HCl"]
    CultureMech_006014 --> ing7_HCl
    CHEBI_17883["CHEBI:17883"]
    ing7_HCl -.-> CHEBI_17883
    ing8_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_006014 --> ing8_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing8_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing9_ZnCl2["ZnCl2"]
    CultureMech_006014 --> ing9_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing9_ZnCl2 -.-> CHEBI_49976
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_006014 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_H3BO3["H3BO3"]
    CultureMech_006014 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_006014 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_006014 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_006014 --> ing14_NiCl2_x_6_H2O
    CHEBI_53542["CHEBI:53542"]
    ing14_NiCl2_x_6_H2O -.-> CHEBI_53542
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_006014 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing16_NaHCO3["NaHCO3"]
    CultureMech_006014 --> ing16_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing16_NaHCO3 -.-> CHEBI_32139
    ing17_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_006014 --> ing17_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing17_Na2S_x_9_H2O -.-> CHEBI_76209
    ing18_sodium_glutarate["sodium glutarate"]
    CultureMech_006014 --> ing18_sodium_glutarate
    CHEBI_24329["CHEBI:24329"]
    ing18_sodium_glutarate -.-> CHEBI_24329
    ing19_Rumen_fluid["Rumen fluid"]
    CultureMech_006014 --> ing19_Rumen_fluid
    ing20_CO2["CO2"]
    CultureMech_006014 --> ing20_CO2
    CHEBI_16526["CHEBI:16526"]
    ing20_CO2 -.-> CHEBI_16526
    ing21_N2["N2"]
    CultureMech_006014 --> ing21_N2
    CHEBI_17997["CHEBI:17997"]
    ing21_N2 -.-> CHEBI_17997
    ing22_Distilled_water["Distilled water"]
    CultureMech_006014 --> ing22_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing22_Distilled_water -.-> CHEBI_15377

Ingredients (23)

#	Ingredient	Concentration	Ontology	Notes
1	KH2PO4 H2O4P.K 136.084 g/mol	0.199800 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Archived DSMZ Medium 298 contributes 0.20 g KH2PO4 to a 1001 mL DSMZ Medium 298 base formulation, yielding 0.199800 g/L before Archived DSMZ Medium 562 replaces butanediol and adds rumen fluid.
2	NH4Cl Cl.H4N 53.492 g/mol	0.249750 G_PER_L	CHEBI:31206 ammonium chloride	Archived DSMZ Medium 298 contributes 0.25 g NH4Cl to a 1001 mL DSMZ Medium 298 base formulation, yielding 0.249750 g/L before Archived DSMZ Medium 562 replaces butanediol and adds rumen fluid.
3	NaCl Cl.Na 58.443 g/mol	0.999001 G_PER_L	CHEBI:26710 sodium chloride	Archived DSMZ Medium 298 contributes 1.00 g NaCl to a 1001 mL DSMZ Medium 298 base formulation, yielding 0.999001 g/L before Archived DSMZ Medium 562 replaces butanediol and adds rumen fluid.
4	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	0.399600 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	Archived DSMZ Medium 298 contributes 0.40 g MgCl2 x 6 H2O to a 1001 mL DSMZ Medium 298 base formulation, yielding 0.399600 g/L before Archived DSMZ Medium 562 replaces butanediol and adds rumen fluid.
5	KCl Cl.K 74.551 g/mol	0.499500 G_PER_L	CHEBI:32588 potassium chloride	Archived DSMZ Medium 298 contributes 0.50 g KCl to a 1001 mL DSMZ Medium 298 base formulation, yielding 0.499500 g/L before Archived DSMZ Medium 562 replaces butanediol and adds rumen fluid.
6	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.149850 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Archived DSMZ Medium 298 contributes 0.15 g CaCl2 x 2 H2O to a 1001 mL DSMZ Medium 298 base formulation, yielding 0.149850 g/L before Archived DSMZ Medium 562 replaces butanediol and adds rumen fluid.
7	Resazurin C12H7NO4 229.191 g/mol	0.000999 G_PER_L	CHEBI:8806 Resazurin	Archived DSMZ Medium 298 contributes 1.00 mg Resazurin to a 1001 mL DSMZ Medium 298 base formulation, yielding 0.000999 g/L before Archived DSMZ Medium 562 replaces butanediol and adds rumen fluid.
8	HCl HCl 36.461 g/mol	0.002498 G_PER_L	CHEBI:17883 hydrogen chloride	Archived DSMZ Medium 298 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1001 mL DSMZ Medium 298 base formulation; the SL-10 stock contains 2.50 g/L HCl, yielding 0.002498 g/L.
9	FeCl2 x 4 H2O Cl2Fe.4H2O 198.811 g/mol	0.001499 G_PER_L	CHEBI:86249 iron dichloride tetrahydrate	Archived DSMZ Medium 298 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1001 mL DSMZ Medium 298 base formulation; the SL-10 stock contains 1.50 g/L FeCl2 x 4 H2O, yielding 0.001499 g/L.
10	ZnCl2 2Cl.Zn 136.296 g/mol	0.0000699 G_PER_L	CHEBI:49976 zinc dichloride	Archived DSMZ Medium 298 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1001 mL DSMZ Medium 298 base formulation; the SL-10 stock contains 0.070 g/L ZnCl2, yielding 0.0000699 g/L.
11	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.0000999 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	Archived DSMZ Medium 298 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1001 mL DSMZ Medium 298 base formulation; the SL-10 stock contains 0.100 g/L MnCl2 x 4 H2O, yielding 0.0000999 g/L.
12	H3BO3 H3BO3 61.833 g/mol	0.00000599 G_PER_L	CHEBI:33118 boric acid	Archived DSMZ Medium 298 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1001 mL DSMZ Medium 298 base formulation; the SL-10 stock contains 0.006 g/L H3BO3, yielding 0.00000599 g/L.
13	CoCl2 x 6 H2O 2Cl.Co.6H2O 237.929 g/mol	0.000190 G_PER_L	CHEBI:53503 cobalt chloride hexahydrate	Archived DSMZ Medium 298 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1001 mL DSMZ Medium 298 base formulation; the SL-10 stock contains 0.190 g/L CoCl2 x 6 H2O, yielding 0.000190 g/L.
14	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	0.00000200 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	Archived DSMZ Medium 298 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1001 mL DSMZ Medium 298 base formulation; the SL-10 stock contains 0.002 g/L CuCl2 x 2 H2O, yielding 0.00000200 g/L.
15	NiCl2 x 6 H2O 2Cl.6H2O.Ni 237.689 g/mol	0.0000240 G_PER_L	CHEBI:53542 nickel chloride hexahydrate	Archived DSMZ Medium 298 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1001 mL DSMZ Medium 298 base formulation; the SL-10 stock contains 0.024 g/L NiCl2 x 6 H2O, yielding 0.0000240 g/L.
16	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.0000360 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Archived DSMZ Medium 298 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1001 mL DSMZ Medium 298 base formulation; the SL-10 stock contains 0.036 g/L Na2MoO4 x 2 H2O, yielding 0.0000360 g/L.
17	NaHCO3 CHO3.Na 84.006 g/mol	2.497502 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Archived DSMZ Medium 298 contributes 2.50 g NaHCO3 to a 1001 mL DSMZ Medium 298 base formulation, yielding 2.497502 g/L before Archived DSMZ Medium 562 replaces butanediol and adds rumen fluid.
18	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.359640 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	Archived DSMZ Medium 298 contributes 0.36 g Na2S x 9 H2O to a 1001 mL DSMZ Medium 298 base formulation, yielding 0.359640 g/L before Archived DSMZ Medium 562 replaces butanediol and adds rumen fluid.
19	sodium glutarate	2.600000 G_PER_L	CHEBI:24329 glutarate	Archived DSMZ Medium 562 replaces butanediol with 2.6 g/L sodium glutarate.
20	Rumen fluid	20.000 ML_PER_L		Archived DSMZ Medium 562 adds 2% v/v rumen fluid to DSMZ Medium 298.
21	CO2 CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Archived DSMZ Medium 298 prepares the medium under 80% N2 and 20% CO2.
22	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Archived DSMZ Medium 298 prepares the medium under 80% N2 and 20% CO2.
23	Distilled water H2O 18.015 g/mol	1000.000 ML_PER_L	CHEBI:15377 water	Archived DSMZ Medium 298 lists 1000 mL distilled water before adding the SL-10 trace solution and later anaerobic stock solutions.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:03.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 562
2026-03-13T06:19:37.653718Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:006014
2026-03-17T19:45:54.463579+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "GLUTARATE medium" to "glutarate_medium" for consistent matching
2026-08-06T08:58:32.440457+00:00	curate_flag_empty_composition.py	FLAGGED_INCOMPLETE_COMPOSITION	Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175).
2026-09-10T00:00:00-07:00	repair_komodo_562_score35.py	RESOLVED_KOMODO_562_SCORE35	Archived DSMZ Medium 562 defines GLUTARATE MEDIUM as DSMZ Medium 298 with butanediol replaced by 2.6 g/L sodium glutarate and with 2% v/v rumen fluid; this record expands archived DSMZ Media 298, 320, and 562 into final per-liter components.