Overview
- External ontology
- komodo.medium:619 (source link unavailable) — HYPHOMICROBIUM METHYLOVORUM MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: KOMODO ModelSEED | ID: 619 | DSMZ Medium: 619 (mediadive.medium:619) | Aerobic: No
Composition
flowchart LR
CultureMech_006113["hyphomicrobium_methylovorum_medium"]
ing0_Methanol["Methanol"]
CultureMech_006113 --> ing0_Methanol
CHEBI_17790["CHEBI:17790"]
ing0_Methanol -.-> CHEBI_17790
ing1__NH4_2HPO4["(NH4)2HPO4"]
CultureMech_006113 --> ing1__NH4_2HPO4
CHEBI_63051["CHEBI:63051"]
ing1__NH4_2HPO4 -.-> CHEBI_63051
ing2_NaCl["NaCl"]
CultureMech_006113 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006113 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_006113 --> ing4_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
ing5_MnSO4_x_1_H2O["MnSO4 x 1 H2O"]
CultureMech_006113 --> ing5_MnSO4_x_1_H2O
CHEBI_86360["CHEBI:86360"]
ing5_MnSO4_x_1_H2O -.-> CHEBI_86360
ing6_Agar["Agar"]
CultureMech_006113 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_Tap_water["Tap water"]
CultureMech_006113 --> ing7_Tap_water
CHEBI_15377["CHEBI:15377"]
ing7_Tap_water -.-> CHEBI_15377
ing8_Biotin["Biotin"]
CultureMech_006113 --> ing8_Biotin
CHEBI_15956["CHEBI:15956"]
ing8_Biotin -.-> CHEBI_15956
ing9_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_006113 --> ing9_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing9_Calcium_pantothenate -.-> CHEBI_31345
ing10_Folic_acid["Folic acid"]
CultureMech_006113 --> ing10_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing10_Folic_acid -.-> CHEBI_27470
ing11_Inositol["Inositol"]
CultureMech_006113 --> ing11_Inositol
CHEBI_24848["CHEBI:24848"]
ing11_Inositol -.-> CHEBI_24848
ing12_Niacin["Niacin"]
CultureMech_006113 --> ing12_Niacin
CHEBI_15940["CHEBI:15940"]
ing12_Niacin -.-> CHEBI_15940
ing13_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_006113 --> ing13_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing13_p_Aminobenzoic_acid -.-> CHEBI_30753
ing14_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_006113 --> ing14_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing14_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing15_Riboflavin["Riboflavin"]
CultureMech_006113 --> ing15_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing15_Riboflavin -.-> CHEBI_17015
ing16_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_006113 --> ing16_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing16_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing17_Choline["Choline"]
CultureMech_006113 --> ing17_Choline
CHEBI_15354["CHEBI:15354"]
ing17_Choline -.-> CHEBI_15354
ing18_Vitamin_B12["Vitamin B12"]
CultureMech_006113 --> ing18_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing18_Vitamin_B12 -.-> CHEBI_176843
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Methanol
CH4O 32.042 g/mol
|
7.92 G_PER_L |
CHEBI:17790
methanol
|
|
| 2 |
(NH4)2HPO4
2H4N.HO4P 132.056 g/mol
|
3 G_PER_L |
CHEBI:63051
diammonium hydrogen phosphate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.01 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 6 |
MnSO4 x 1 H2O
Mn.O4S 151.001 g/mol
|
0.005 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 7 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 8 |
Tap water
H2O 18.015 g/mol
|
1000 G_PER_L |
CHEBI:15377
water
|
|
| 9 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.0002 G_PER_L |
CHEBI:15956
biotin
|
|
| 10 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.04 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 11 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.0002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 12 |
Inositol
C6H12O6 180.156 g/mol
|
0.2 G_PER_L |
CHEBI:24848
inositol
|
|
| 13 |
Niacin
C6H5NO2 123.111 g/mol
|
0.04 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 14 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.02 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 15 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.04 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 16 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.04 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 17 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.02 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 18 |
Choline
C5H14NO 104.173 g/mol
|
0.1 G_PER_L |
CHEBI:15354
choline
|
|
| 19 | Vitamin B12 | 2e-06 G_PER_L |
CHEBI:176843
vitamin B12
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 619 |
| 2026-02-03T11:19:13.718365 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 619 |
| 2026-03-13T06:19:38.373609Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006113 |
| 2026-03-17T06:30:38.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:55.281869+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HYPHOMICROBIUM METHYLOVORUM MEDIUM" to "hyphomicrobium_methylovorum_medium" for consistent matching |
| 2026-06-05T07:40:24.979095+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.286991+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:45.401661+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |