Overview
- External ontology
- komodo.medium:63.1 (source link unavailable) — For DSM 12927 supplement medium with 10 g/l NaCl
- Applications
-
- Microbial cultivation
- pH
- 7.8
- Notes
- pH buffer: NaOH | Source: KOMODO ModelSEED | ID: 63.1 | DSMZ Medium: 63 (mediadive.medium:63) | Aerobic: Yes
Composition
flowchart LR
CultureMech_006137["for_dsm_12927_supplement_medium_with_10_g_l_nacl"]
ing0_K2HPO4["K2HPO4"]
CultureMech_006137 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_NH4Cl["NH4Cl"]
CultureMech_006137 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_Na2SO4["Na2SO4"]
CultureMech_006137 --> ing2_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing2_Na2SO4 -.-> CHEBI_32149
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_006137 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006137 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_Na_acetate["Na-acetate"]
CultureMech_006137 --> ing5_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing5_Na_acetate -.-> CHEBI_32954
ing6_Na_pyruvate["Na-pyruvate"]
CultureMech_006137 --> ing6_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing6_Na_pyruvate -.-> CHEBI_50144
ing7_Yeast_extract["Yeast extract"]
CultureMech_006137 --> ing7_Yeast_extract
ing8_Sodium_resazurin["Sodium resazurin"]
CultureMech_006137 --> ing8_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Sodium_resazurin -.-> CHEBI_8806
ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_006137 --> ing9_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
ing10_Na_thioglycolate["Na-thioglycolate"]
CultureMech_006137 --> ing10_Na_thioglycolate
CHEBI_86481["CHEBI:86481"]
ing10_Na_thioglycolate -.-> CHEBI_86481
ing11_Ascorbic_acid["Ascorbic acid"]
CultureMech_006137 --> ing11_Ascorbic_acid
CHEBI_22652["CHEBI:22652"]
ing11_Ascorbic_acid -.-> CHEBI_22652
ing12_NaOH["NaOH"]
CultureMech_006137 --> ing12_NaOH
CHEBI_32145["CHEBI:32145"]
ing12_NaOH -.-> CHEBI_32145
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.510204 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.02041 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
Na2SO4
2Na.O4S 142.043 g/mol
|
1.02041 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.102041 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
2.04082 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
2.04082 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 7 |
Na-pyruvate
C3H3O3.Na 110.044 g/mol
|
5.10204 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 8 | Yeast extract | 1.02041 G_PER_L | ||
| 9 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 10 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
50 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 11 |
Na-thioglycolate
C2H3O2S.Na 114.101 g/mol
|
10 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
|
| 12 | Ascorbic acid | 10 G_PER_L |
CHEBI:22652
ascorbic acid
|
|
| 13 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 63.1 |
| 2026-02-03T11:19:00.581829 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 63 |
| 2026-03-13T03:08:53.350257Z | data-quality-cleanup-v1.0 | Data quality fixes | Added pH buffer: NaOH |
| 2026-03-13T06:19:38.552105Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006137 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-17T06:29:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:55.492348+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "For DSM 12927 supplement medium with 10 g/l NaCl" to "for_dsm_12927_supplement_medium_with_10_g_l_nacl" for consistent matching |
| 2026-05-16T09:03:32.450870+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:01.092607+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:57.128906+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |