Overview
- External ontology
- komodo.medium:638 (source link unavailable) — LACTOBACILLUS medium III
- Applications
-
- Microbial cultivation
- pH
- 6.3
- Notes
- Source: KOMODO ModelSEED | ID: 638 | DSMZ Medium: 638 (mediadive.medium:638) | Aerobic: No
Composition
flowchart LR
CultureMech_006159["lactobacillus_medium_iii"]
ing0_Tryptone["Tryptone"]
CultureMech_006159 --> ing0_Tryptone
ing1_Meat_extract["Meat extract"]
CultureMech_006159 --> ing1_Meat_extract
ing2_Yeast_extract["Yeast extract"]
CultureMech_006159 --> ing2_Yeast_extract
ing3_Glucose["Glucose"]
CultureMech_006159 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Fructose["Fructose"]
CultureMech_006159 --> ing4_Fructose
CHEBI_28757["CHEBI:28757"]
ing4_Fructose -.-> CHEBI_28757
ing5_Maltose["Maltose"]
CultureMech_006159 --> ing5_Maltose
CHEBI_17306["CHEBI:17306"]
ing5_Maltose -.-> CHEBI_17306
ing6_Na_gluconate["Na-gluconate"]
CultureMech_006159 --> ing6_Na_gluconate
CHEBI_84997["CHEBI:84997"]
ing6_Na_gluconate -.-> CHEBI_84997
ing7_Na_acetate_x_3_H2O["Na-acetate x 3 H2O"]
CultureMech_006159 --> ing7_Na_acetate_x_3_H2O
CHEBI_32138["CHEBI:32138"]
ing7_Na_acetate_x_3_H2O -.-> CHEBI_32138
ing8__NH4_2_citrate["(NH4)2 citrate"]
CultureMech_006159 --> ing8__NH4_2_citrate
CHEBI_63076["CHEBI:63076"]
ing8__NH4_2_citrate -.-> CHEBI_63076
ing9_K2HPO4_x_3_H2O["K2HPO4 x 3 H2O"]
CultureMech_006159 --> ing9_K2HPO4_x_3_H2O
CHEBI_131527["CHEBI:131527"]
ing9_K2HPO4_x_3_H2O -.-> CHEBI_131527
ing10_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006159 --> ing10_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing10_MgSO4_x_7_H2O -.-> CHEBI_31795
ing11_MnSO4_x_4_H2O["MnSO4 x 4 H2O"]
CultureMech_006159 --> ing11_MnSO4_x_4_H2O
CHEBI_86358["CHEBI:86358"]
ing11_MnSO4_x_4_H2O -.-> CHEBI_86358
ing12_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_006159 --> ing12_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing12_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing13_Tween_80["Tween 80"]
CultureMech_006159 --> ing13_Tween_80
CHEBI_53426["CHEBI:53426"]
ing13_Tween_80 -.-> CHEBI_53426
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tryptone | 10 G_PER_L | ||
| 2 | Meat extract | 5 G_PER_L | ||
| 3 | Yeast extract | 5 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
7 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 | Fructose | 7 G_PER_L |
CHEBI:28757
fructose
|
|
| 6 |
Maltose
C12H22O11 342.297 g/mol
|
7 G_PER_L |
CHEBI:17306
maltose
|
|
| 7 |
Na-gluconate
C6H11O7.Na 218.137 g/mol
|
2 G_PER_L |
CHEBI:84997
sodium gluconate
|
|
| 8 |
Na-acetate x 3 H2O
C2H3O2.3H2O.Na 136.079 g/mol
|
5 G_PER_L |
CHEBI:32138
sodium acetate trihydrate
|
|
| 9 |
(NH4)2 citrate
C6H6O7.2H4N 226.185 g/mol
|
2 G_PER_L |
CHEBI:63076
diammonium citrate
|
|
| 10 |
K2HPO4 x 3 H2O
HO4P.2K 174.174 g/mol
|
2.6 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 11 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 12 |
MnSO4 x 4 H2O
4H2O.Mn.O4S 223.061 g/mol
|
0.05 G_PER_L |
CHEBI:86358
manganese(II) sulfate tetrahydrate
|
|
| 13 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 14 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 638 |
| 2026-02-03T11:19:03.956237 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 638 |
| 2026-03-13T06:19:38.679679Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006159 |
| 2026-03-17T06:30:07.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:55.642986+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LACTOBACILLUS medium III" to "lactobacillus_medium_iii" for consistent matching |
| 2026-06-05T07:40:25.129149+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:45.442530+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-08T03:14:00.696534+00:00 | codex | CURATED_KOMODO_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. KOMODO accession 638 is mapped to the same DSMZ accession by https://komodo.modelseed.org/servlet/KomodoTomcatServerSideUtilitiesModelSeed?MediaList; mapping response SHA256=d8259d98e6dbffdf6961a1ab92bc86f3567d5d96de352bfa079edb2a91e9580a. The mapping supports identity only, not ingredient quantities or variants. |