clostridium_estertheticum_medium (CultureMech:006182)

Overview

External ontology

komodo.medium:642 (source link unavailable) — CLOSTRIDIUM ESTERTHETICUM medium

Applications

Microbial cultivation

pH

6.8

Notes

Archived DSMZ Medium 642 defines CLOSTRIDIUM ESTERTHETICUM MEDIUM as RCM medium prepared under 80% N2 + 20% CO2, with 2 g/L sodium bicarbonate and 1% glucose added before use from anaerobic stock solutions; this record expands the KOMODO Medium 642 RCM component table and adds the DSMZ bicarbonate, glucose, and anaerobic gas components.

Composition

flowchart LR
    CultureMech_006182["clostridium_estertheticum_medium"]
    ing0_sodium_bicarbonate["sodium bicarbonate"]
    CultureMech_006182 --> ing0_sodium_bicarbonate
    CHEBI_32139["CHEBI:32139"]
    ing0_sodium_bicarbonate -.-> CHEBI_32139
    ing1_Agar["Agar"]
    CultureMech_006182 --> ing1_Agar
    CHEBI_2509["CHEBI:2509"]
    ing1_Agar -.-> CHEBI_2509
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_006182 --> ing2_Yeast_extract
    ing3_Glucose["Glucose"]
    CultureMech_006182 --> ing3_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing3_Glucose -.-> CHEBI_17634
    ing4_CO2["CO2"]
    CultureMech_006182 --> ing4_CO2
    CHEBI_16526["CHEBI:16526"]
    ing4_CO2 -.-> CHEBI_16526
    ing5_Sodium_acetate["Sodium acetate"]
    CultureMech_006182 --> ing5_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing5_Sodium_acetate -.-> CHEBI_32954
    ing6_N2["N2"]
    CultureMech_006182 --> ing6_N2
    CHEBI_17997["CHEBI:17997"]
    ing6_N2 -.-> CHEBI_17997
    ing7_Cysteine_hydrochloride["Cysteine hydrochloride"]
    CultureMech_006182 --> ing7_Cysteine_hydrochloride
    CHEBI_91247["CHEBI:91247"]
    ing7_Cysteine_hydrochloride -.-> CHEBI_91247
    ing8_Lab_Lemco_powder["Lab-Lemco powder"]
    CultureMech_006182 --> ing8_Lab_Lemco_powder
    ing9_Sodium_chloride["Sodium chloride"]
    CultureMech_006182 --> ing9_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing9_Sodium_chloride -.-> CHEBI_26710
    ing10_Soluble_starch["Soluble starch"]
    CultureMech_006182 --> ing10_Soluble_starch
    CHEBI_28017["CHEBI:28017"]
    ing10_Soluble_starch -.-> CHEBI_28017
    ing11_Peptone["Peptone"]
    CultureMech_006182 --> ing11_Peptone

Ingredients (12)

#	Ingredient	Concentration	Ontology	Notes
1	sodium bicarbonate CHO3.Na 84.006 g/mol	2.000000 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Archived DSMZ Medium 642 adds 2 g/L sodium bicarbonate before use.
2	Agar	0.500000 G_PER_L	CHEBI:2509 agar	KOMODO Medium 642 expands the RCM base and lists 0.50 g/L agar.
3	Yeast extract	3.000000 G_PER_L	FOODON:03315426 yeast extract	KOMODO Medium 642 expands the RCM base and lists 3.00 g/L yeast extract.
4	Glucose C6H12O6 180.156 g/mol	15.000000 G_PER_L	CHEBI:17634 D-glucose	KOMODO Medium 642 expands total glucose to 15 g/L, corresponding to the RCM base glucose plus the 1% anaerobic glucose stock addition specified by Archived DSMZ Medium 642.
5	CO2 CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Archived DSMZ Medium 642 prepares the RCM base under 80% N2 + 20% CO2 gas.
6	Sodium acetate C2H3O2.Na 82.034 g/mol	3.000000 G_PER_L	CHEBI:32954 sodium acetate	KOMODO Medium 642 expands the RCM base and lists 3.00 g/L sodium acetate.
7	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Archived DSMZ Medium 642 prepares the RCM base under 80% N2 + 20% CO2 gas.
8	Cysteine hydrochloride C3H7NO2S.HCl 157.622 g/mol	0.500000 G_PER_L	CHEBI:91247 L-cysteine hydrochloride	KOMODO Medium 642 expands the RCM base and lists 0.50 g/L cysteine hydrochloride.
9	Lab-Lemco powder	10.000000 G_PER_L	FOODON:03302088 beef extract	KOMODO Medium 642 expands the RCM base and lists 10.00 g/L Lab-Lemco powder.
10	Sodium chloride Cl.Na 58.443 g/mol	5.000000 G_PER_L	CHEBI:26710 sodium chloride	KOMODO Medium 642 expands the RCM base and lists 5.00 g/L sodium chloride.
11	Soluble starch	1.000000 G_PER_L	CHEBI:28017 starch	KOMODO Medium 642 expands the RCM base and lists 1.00 g/L soluble starch.
12	Peptone	10.000000 G_PER_L	MICRO:0000178 (source link unavailable) peptone	KOMODO Medium 642 expands the RCM base and lists 10.00 g/L peptone.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:03.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 642
2026-03-13T06:19:38.905524Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:006182
2026-03-17T19:45:55.911517+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "CLOSTRIDIUM ESTERTHETICUM medium" to "clostridium_estertheticum_medium" for consistent matching
2026-08-06T08:58:32.407922+00:00	curate_flag_empty_composition.py	FLAGGED_INCOMPLETE_COMPOSITION	Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175).
2026-09-10T00:00:00-07:00	repair_komodo_642_score30.py	RESOLVED_KOMODO_642_SCORE30	Archived DSMZ Medium 642 defines CLOSTRIDIUM ESTERTHETICUM MEDIUM as RCM medium prepared under 80% N2 + 20% CO2, with 2 g/L sodium bicarbonate and 1% glucose added before use from anaerobic stock solutions; this record expands the KOMODO Medium 642 RCM component table and adds the DSMZ bicarbonate, glucose, and anaerobic gas components.
2026-09-22T00:03:26.686152+00:00	repair_rejected_peptone_groundings.py	CORRECTED_REJECTED_PEPTONE_GROUNDING	CultureMech #484. /ingredients/11/term/id: Peptone FOODON:03302071 -> MICRO:0000178 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved.