medium_645_modified_for_dsm_44482

CultureMech:006187 bacterial COMPLEX SOLID_AGAR
Original name: MEDIUM 645 MODIFIED FOR DSM 44482

Overview

External ontology
komodo.medium:645_44482 (source link unavailable) — MEDIUM 645 MODIFIED FOR DSM 44482
Applications
  • Microbial cultivation
Notes
Source: KOMODO ModelSEED | ID: 645_44482 | DSMZ Medium: 645 (mediadive.medium:645) | Aerobic: No Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. This record now follows its own KOMODO table rather than the composition copied from DSMZ. Rates remain provider-qualified; a dilution mechanism is unresolved. Null PH Info is not replaced by a copied pH assertion. No source omission proves an experimentally absent ingredient. Source-specific ingredient identities, quantity bases and preparation routes supersede the copied flattened amounts. Historical events are preserved. Chemical groundings are not comprehensively recertified; no taxa-media association is added.

Composition

flowchart LR
    CultureMech_006187["medium_645_modified_for_dsm_44482"]
    ing0_L_Glutamic_acid["L-Glutamic acid"]
    CultureMech_006187 --> ing0_L_Glutamic_acid
    CHEBI_16015["CHEBI:16015"]
    ing0_L_Glutamic_acid -.-> CHEBI_16015
    ing1_CuSO4["CuSO4"]
    CultureMech_006187 --> ing1_CuSO4
    CHEBI_23414["CHEBI:23414"]
    ing1_CuSO4 -.-> CHEBI_23414
    ing2_Na2HPO4["Na2HPO4"]
    CultureMech_006187 --> ing2_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing2_Na2HPO4 -.-> CHEBI_34683
    ing3_ZnSO4["ZnSO4"]
    CultureMech_006187 --> ing3_ZnSO4
    CHEBI_35176["CHEBI:35176"]
    ing3_ZnSO4 -.-> CHEBI_35176
    ing4_Sodium_citrate["Sodium citrate"]
    CultureMech_006187 --> ing4_Sodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing4_Sodium_citrate -.-> CHEBI_53258
    ing5_Distilled_water["Distilled water"]
    CultureMech_006187 --> ing5_Distilled_water
    ing6_Agar["Agar"]
    CultureMech_006187 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Glycerol["Glycerol"]
    CultureMech_006187 --> ing7_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing7_Glycerol -.-> CHEBI_17754
    ing8_KH2PO4["KH2PO4"]
    CultureMech_006187 --> ing8_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing8_KH2PO4 -.-> CHEBI_63036
    ing9_Pyridoxine["Pyridoxine"]
    CultureMech_006187 --> ing9_Pyridoxine
    CHEBI_16709["CHEBI:16709"]
    ing9_Pyridoxine -.-> CHEBI_16709
    ing10_Biotin["Biotin"]
    CultureMech_006187 --> ing10_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing10_Biotin -.-> CHEBI_15956
    ing11_Dextrose["Dextrose"]
    CultureMech_006187 --> ing11_Dextrose
    CHEBI_17634["CHEBI:17634"]
    ing11_Dextrose -.-> CHEBI_17634
    ing12_MgSO4["MgSO4"]
    CultureMech_006187 --> ing12_MgSO4
    CHEBI_32599["CHEBI:32599"]
    ing12_MgSO4 -.-> CHEBI_32599
    ing13_Catalase["Catalase"]
    CultureMech_006187 --> ing13_Catalase
    ing14_Ferric_ammonium_citrate["Ferric ammonium citrate"]
    CultureMech_006187 --> ing14_Ferric_ammonium_citrate
    CHEBI_31604["CHEBI:31604"]
    ing14_Ferric_ammonium_citrate -.-> CHEBI_31604
    ing15_Oleic_acid["Oleic acid"]
    CultureMech_006187 --> ing15_Oleic_acid
    CHEBI_16196["CHEBI:16196"]
    ing15_Oleic_acid -.-> CHEBI_16196
    ing16_CaCl2["CaCl2"]
    CultureMech_006187 --> ing16_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing16_CaCl2 -.-> CHEBI_3312
    ing17_Albumin_fraction_V__bovine["Albumin fraction V, bovine"]
    CultureMech_006187 --> ing17_Albumin_fraction_V__bovine
    ing18_NaCl["NaCl"]
    CultureMech_006187 --> ing18_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing18_NaCl -.-> CHEBI_26710
    ing19__NH4_2SO4["(NH4)2SO4"]
    CultureMech_006187 --> ing19__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing19__NH4_2SO4 -.-> CHEBI_62946
    ing20_Malachite_green["Malachite green"]
    CultureMech_006187 --> ing20_Malachite_green
    CHEBI_72449["CHEBI:72449"]
    ing20_Malachite_green -.-> CHEBI_72449

Ingredients (21)

# Ingredient Concentration Ontology Notes
1 L-Glutamic acid
C5H9NO4 147.13 g/mol
0.25 G_PER_L CHEBI:16015
L-glutamic acid
 Provider-reported medium rate: Gram Amount 0.25 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form.
2 CuSO4
Cu.O4S 159.609 g/mol
4.99E-4 G_PER_L CHEBI:23414
copper(II) sulfate
 Provider-reported medium rate: Gram Amount 4.99E-4 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form.
3 Na2HPO4
HO4P.2Na 141.958 g/mol
0.75 G_PER_L CHEBI:34683
disodium hydrogenphosphate
 Provider-reported medium rate: Gram Amount 0.75 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form. Source secondary-name ambiguity: displayed name 'Na2HPO4' versus 'sodium phosphate monobasique'; retained chemical annotations are not recertified.
4 ZnSO4
O4S.Zn 161.453 g/mol
4.99E-4 G_PER_L CHEBI:35176
zinc sulfate
 Provider-reported medium rate: Gram Amount 4.99E-4 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form.
5 Sodium citrate
C6H5O7.3Na 258.069 g/mol
0.20 G_PER_L CHEBI:53258
sodium citrate
 Provider-reported medium rate: Gram Amount 0.20 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form.
6 Distilled water The source Gram Amount is null: no fixed amount is asserted; missing is not zero. The Mol Amount column is not used to infer a new molarity or hydrate form.
7 Agar 7.48 G_PER_L CHEBI:2509
agar
 Provider-reported medium rate: Gram Amount 7.48 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form.
8 Glycerol
C3H8O3 92.094 g/mol
CHEBI:17754
glycerol
 The source Gram Amount is null: no fixed amount is asserted; missing is not zero. The Mol Amount column is not used to infer a new molarity or hydrate form.
9 KH2PO4
H2O4P.K 136.084 g/mol
0.75 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Provider-reported medium rate: Gram Amount 0.75 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form.
10 Pyridoxine
C8H11NO3 169.18 g/mol
4.99E-4 G_PER_L CHEBI:16709
pyridoxine
 Provider-reported medium rate: Gram Amount 4.99E-4 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form. Source secondary-name ambiguity: displayed name 'Pyridoxine' versus 'Pyridoxine HCl'; retained chemical annotations are not recertified.
11 Biotin
C10H16N2O3S 244.316 g/mol
2.49E-4 G_PER_L CHEBI:15956
biotin
 Provider-reported medium rate: Gram Amount 2.49E-4 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form.
12 Dextrose
C6H12O6 180.156 g/mol
1.00 G_PER_L CHEBI:17634
D-glucose
 Provider-reported medium rate: Gram Amount 1.00 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form.
13 MgSO4
Mg.O4S 120.368 g/mol
0.01 G_PER_L CHEBI:32599
magnesium sulfate
 Provider-reported medium rate: Gram Amount 0.01 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form.
14 Catalase 2.00E-3 G_PER_L Provider-reported medium rate: Gram Amount 2.00E-3 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form.
15 Ferric ammonium citrate 0.02 G_PER_L CHEBI:31604
ferric ammonium citrate
 Provider-reported medium rate: Gram Amount 0.02 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form.
16 Oleic acid
C18H34O2 282.468 g/mol
0.02 G_PER_L CHEBI:16196
oleic acid
 Provider-reported medium rate: Gram Amount 0.02 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form.
17 CaCl2
Ca.2Cl 110.984 g/mol
2.49E-4 G_PER_L CHEBI:3312
calcium dichloride
 Provider-reported medium rate: Gram Amount 2.49E-4 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form.
18 Albumin fraction V, bovine 2.49 G_PER_L Provider-reported medium rate: Gram Amount 2.49 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form.
19 NaCl
Cl.Na 58.443 g/mol
0.42 G_PER_L CHEBI:26710
sodium chloride
 Provider-reported medium rate: Gram Amount 0.42 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form.
20 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.25 G_PER_L CHEBI:62946
ammonium sulfate
 Provider-reported medium rate: Gram Amount 0.25 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form.
21 Malachite green
C23H25N2.Cl 364.92 g/mol
1.25E-4 G_PER_L CHEBI:72449
malachite green
 Provider-reported medium rate: Gram Amount 1.25E-4 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-14T05:58:58.561437+00:00 idnf-grounding-fix-v1.0 Fixed ID_NOT_FOUND term groundings Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable).
2026-01-27T01:15:03.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 645_44482
2026-02-03T11:19:03.586663 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 645
2026-03-13T06:19:38.953945Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:006187
2026-03-17T06:30:06.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:55.970539+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MEDIUM 645 MODIFIED FOR DSM 44482" to "medium_645_modified_for_dsm_44482" for consistent matching
2026-06-05T07:41:26.393661+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:05.210217+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-09T01:29:53.718683+00:00 codex RESTORED_LINKED_MIDDLEBROOK_FORMULATIONS_AND_VARIANT_SCOPES_WITH_EVIDENCE Restore source-specific Middlebrook ingredients, distinguish commercial/alternative/base/OADC scopes, retain uncertainty and repair reciprocal family links without changing permanent IDs.