Overview
- External ontology
- komodo.medium:645a (source link unavailable) — HEMIN medium FOR MYCOBACTERIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: KOMODO ModelSEED | ID: 645a | DSMZ Medium: 645a (mediadive.medium:645a) | Aerobic: No Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. This record now follows its own KOMODO table rather than the composition copied from DSMZ. Rates remain provider-qualified; a dilution mechanism is unresolved. Null PH Info is not replaced by a copied pH assertion. No source omission proves an experimentally absent ingredient. The hemin variant is not an exact duplicate of the plain 645 records. Its glycerol omission is a table observation, not evidence that the experimental preparation was glycerol-free. Source-specific ingredient identities, quantity bases and preparation routes supersede the copied flattened amounts. Historical events are preserved. Chemical groundings are not comprehensively recertified; no taxa-media association is added.
Composition
flowchart LR
CultureMech_006191["hemin_medium_for_mycobacterium"]
ing0_L_Glutamic_acid["L-Glutamic acid"]
CultureMech_006191 --> ing0_L_Glutamic_acid
CHEBI_16015["CHEBI:16015"]
ing0_L_Glutamic_acid -.-> CHEBI_16015
ing1_CuSO4["CuSO4"]
CultureMech_006191 --> ing1_CuSO4
CHEBI_23414["CHEBI:23414"]
ing1_CuSO4 -.-> CHEBI_23414
ing2_Na2HPO4["Na2HPO4"]
CultureMech_006191 --> ing2_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing2_Na2HPO4 -.-> CHEBI_34683
ing3_ZnSO4["ZnSO4"]
CultureMech_006191 --> ing3_ZnSO4
CHEBI_35176["CHEBI:35176"]
ing3_ZnSO4 -.-> CHEBI_35176
ing4_Sodium_citrate["Sodium citrate"]
CultureMech_006191 --> ing4_Sodium_citrate
CHEBI_53258["CHEBI:53258"]
ing4_Sodium_citrate -.-> CHEBI_53258
ing5_Distilled_water["Distilled water"]
CultureMech_006191 --> ing5_Distilled_water
ing6_NAOH["NAOH"]
CultureMech_006191 --> ing6_NAOH
ing7_Agar["Agar"]
CultureMech_006191 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
ing8_KH2PO4["KH2PO4"]
CultureMech_006191 --> ing8_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing8_KH2PO4 -.-> CHEBI_63036
ing9_Pyridoxine["Pyridoxine"]
CultureMech_006191 --> ing9_Pyridoxine
CHEBI_16709["CHEBI:16709"]
ing9_Pyridoxine -.-> CHEBI_16709
ing10_Biotin["Biotin"]
CultureMech_006191 --> ing10_Biotin
CHEBI_15956["CHEBI:15956"]
ing10_Biotin -.-> CHEBI_15956
ing11_Dextrose["Dextrose"]
CultureMech_006191 --> ing11_Dextrose
CHEBI_17634["CHEBI:17634"]
ing11_Dextrose -.-> CHEBI_17634
ing12_MgSO4["MgSO4"]
CultureMech_006191 --> ing12_MgSO4
CHEBI_32599["CHEBI:32599"]
ing12_MgSO4 -.-> CHEBI_32599
ing13_Catalase["Catalase"]
CultureMech_006191 --> ing13_Catalase
ing14_Ferric_ammonium_citrate["Ferric ammonium citrate"]
CultureMech_006191 --> ing14_Ferric_ammonium_citrate
CHEBI_31604["CHEBI:31604"]
ing14_Ferric_ammonium_citrate -.-> CHEBI_31604
ing15_Oleic_acid["Oleic acid"]
CultureMech_006191 --> ing15_Oleic_acid
CHEBI_16196["CHEBI:16196"]
ing15_Oleic_acid -.-> CHEBI_16196
ing16_CaCl2["CaCl2"]
CultureMech_006191 --> ing16_CaCl2
CHEBI_3312["CHEBI:3312"]
ing16_CaCl2 -.-> CHEBI_3312
ing17_Albumin_fraction_V__bovine["Albumin fraction V, bovine"]
CultureMech_006191 --> ing17_Albumin_fraction_V__bovine
ing18_NaCl["NaCl"]
CultureMech_006191 --> ing18_NaCl
CHEBI_26710["CHEBI:26710"]
ing18_NaCl -.-> CHEBI_26710
ing19_hemin["hemin"]
CultureMech_006191 --> ing19_hemin
ing20__NH4_2SO4["(NH4)2SO4"]
CultureMech_006191 --> ing20__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing20__NH4_2SO4 -.-> CHEBI_62946
ing21_Malachite_green["Malachite green"]
CultureMech_006191 --> ing21_Malachite_green
CHEBI_72449["CHEBI:72449"]
ing21_Malachite_green -.-> CHEBI_72449
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
L-Glutamic acid
C5H9NO4 147.13 g/mol
|
0.50 G_PER_L |
CHEBI:16015
L-glutamic acid
|
Provider-reported medium rate: Gram Amount 0.50 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form. |
| 2 |
CuSO4
Cu.O4S 159.609 g/mol
|
1.00E-3 G_PER_L |
CHEBI:23414
copper(II) sulfate
|
Provider-reported medium rate: Gram Amount 1.00E-3 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form. |
| 3 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
1.50 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Provider-reported medium rate: Gram Amount 1.50 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form. Source secondary-name ambiguity: displayed name 'Na2HPO4' versus 'sodium phosphate monobasique'; retained chemical annotations are not recertified. |
| 4 |
ZnSO4
O4S.Zn 161.453 g/mol
|
1.00E-3 G_PER_L |
CHEBI:35176
zinc sulfate
|
Provider-reported medium rate: Gram Amount 1.00E-3 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form. |
| 5 |
Sodium citrate
C6H5O7.3Na 258.069 g/mol
|
0.40 G_PER_L |
CHEBI:53258
sodium citrate
|
Provider-reported medium rate: Gram Amount 0.40 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form. |
| 6 | Distilled water | The source Gram Amount is null: no fixed amount is asserted; missing is not zero. The Mol Amount column is not used to infer a new molarity or hydrate form. | ||
| 7 | NAOH | The source Gram Amount is null: no fixed amount is asserted; missing is not zero. The Mol Amount column is not used to infer a new molarity or hydrate form. Original DSMZ 645a says 60 micromoles hemin without a volume denominator, dissolved using 1N NAOH without an addition dose. This does not independently establish 60 uM or a mass conversion. The provider hemin rate and unquantified NaOH dose remain separately scoped. | ||
| 8 | Agar | 15.00 G_PER_L |
CHEBI:2509
agar
|
Provider-reported medium rate: Gram Amount 15.00 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form. |
| 9 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.50 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Provider-reported medium rate: Gram Amount 1.50 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form. |
| 10 |
Pyridoxine
C8H11NO3 169.18 g/mol
|
1.00E-3 G_PER_L |
CHEBI:16709
pyridoxine
|
Provider-reported medium rate: Gram Amount 1.00E-3 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form. Source secondary-name ambiguity: displayed name 'Pyridoxine' versus 'Pyridoxine HCl'; retained chemical annotations are not recertified. |
| 11 |
Biotin
C10H16N2O3S 244.316 g/mol
|
5.00E-4 G_PER_L |
CHEBI:15956
biotin
|
Provider-reported medium rate: Gram Amount 5.00E-4 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form. |
| 12 |
Dextrose
C6H12O6 180.156 g/mol
|
2.00 G_PER_L |
CHEBI:17634
D-glucose
|
Provider-reported medium rate: Gram Amount 2.00 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form. |
| 13 |
MgSO4
Mg.O4S 120.368 g/mol
|
0.03 G_PER_L |
CHEBI:32599
magnesium sulfate
|
Provider-reported medium rate: Gram Amount 0.03 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form. |
| 14 | Catalase | 4.00E-3 G_PER_L | Provider-reported medium rate: Gram Amount 4.00E-3 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form. | |
| 15 | Ferric ammonium citrate | 0.04 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
Provider-reported medium rate: Gram Amount 0.04 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form. |
| 16 |
Oleic acid
C18H34O2 282.468 g/mol
|
0.05 G_PER_L |
CHEBI:16196
oleic acid
|
Provider-reported medium rate: Gram Amount 0.05 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form. |
| 17 |
CaCl2
Ca.2Cl 110.984 g/mol
|
5.00E-4 G_PER_L |
CHEBI:3312
calcium dichloride
|
Provider-reported medium rate: Gram Amount 5.00E-4 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form. |
| 18 | Albumin fraction V, bovine | 5.00 G_PER_L | Provider-reported medium rate: Gram Amount 5.00 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form. | |
| 19 |
NaCl
Cl.Na 58.443 g/mol
|
0.85 G_PER_L |
CHEBI:26710
sodium chloride
|
Provider-reported medium rate: Gram Amount 0.85 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form. |
| 20 | hemin | 0.04 G_PER_L | Provider-reported medium rate: Gram Amount 0.04 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form. Original DSMZ 645a says 60 micromoles hemin without a volume denominator, dissolved using 1N NAOH without an addition dose. This does not independently establish 60 uM or a mass conversion. The provider hemin rate and unquantified NaOH dose remain separately scoped. | |
| 21 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.50 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Provider-reported medium rate: Gram Amount 0.50 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form. |
| 22 |
Malachite green
C23H25N2.Cl 364.92 g/mol
|
2.50E-4 G_PER_L |
CHEBI:72449
malachite green
|
Provider-reported medium rate: Gram Amount 2.50E-4 per liter. Preserve displayed precision; the linked DSMZ batch/stock recipe does not independently explain this rate. The Mol Amount column is not used to infer a new molarity or hydrate form. |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable). |
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 645a |
| 2026-02-03T11:19:08.899481 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 645 |
| 2026-03-13T06:19:38.990269Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006191 |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:56.017752+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HEMIN medium FOR MYCOBACTERIUM" to "hemin_medium_for_mycobacterium" for consistent matching |
| 2026-06-05T07:40:25.168042+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:45.455984+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-09T01:29:53.718683+00:00 | codex | RESTORED_LINKED_MIDDLEBROOK_FORMULATIONS_AND_VARIANT_SCOPES_WITH_EVIDENCE | Restore source-specific Middlebrook ingredients, distinguish commercial/alternative/base/OADC scopes, retain uncertainty and repair reciprocal family links without changing permanent IDs. |