centenum_medium

CultureMech:006227 bacterial COMPLEX LIQUID
Original name: CENTENUM medium

Overview

External ontology
komodo.medium:650 (source link unavailable) — CENTENUM medium
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: KOMODO ModelSEED | ID: 650 | DSMZ Medium: 650 (mediadive.medium:650) | Aerobic: No Concentration follow-up: water volumes denote batch inputs, not final volume. Historical source-duplicate links and import annotations are preserved but not revalidated by this correction; linked records may still carry flattened quantities. Preparation stages follow the cited source.

Composition

flowchart LR
    CultureMech_006227["centenum_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_006227 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_MgSO4["MgSO4"]
    CultureMech_006227 --> ing1_MgSO4
    CHEBI_32599["CHEBI:32599"]
    ing1_MgSO4 -.-> CHEBI_32599
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_006227 --> ing2_Yeast_extract
    ing3_Vitamin_B12["Vitamin B12"]
    CultureMech_006227 --> ing3_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing3_Vitamin_B12 -.-> CHEBI_176843
    ing4_Tap_water["Tap water"]
    CultureMech_006227 --> ing4_Tap_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Tap_water -.-> CHEBI_15377
    ing5_Pyruvate["Pyruvate"]
    CultureMech_006227 --> ing5_Pyruvate
    CHEBI_15361["CHEBI:15361"]
    ing5_Pyruvate -.-> CHEBI_15361

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Source batch input: 1 g. Retained g/L is the provider's nominal main-recipe rate (volume field 1000 ml), not a measured final-medium concentration or a stock concentration.
2 MgSO4
Mg.O4S 120.368 g/mol
0.5 G_PER_L CHEBI:32599
magnesium sulfate
 Source batch input: 0.5 g. Retained g/L is the provider's nominal main-recipe rate (volume field 1000 ml), not a measured final-medium concentration or a stock concentration.
3 Yeast extract 10 G_PER_L Source batch input: 10 g. Retained g/L is the provider's nominal main-recipe rate (volume field 1000 ml), not a measured final-medium concentration or a stock concentration.
4 Vitamin B12 2e-05 G_PER_L CHEBI:176843
vitamin B12
 Source batch input: 20 µg. Retained g/L is the provider's nominal main-recipe rate (volume field 1000 ml), not a measured final-medium concentration or a stock concentration.
5 Tap water
H2O 18.015 g/mol
1 L CHEBI:15377
water
 1000 ml / 1000 = 1 L tap-water input per batch, not a mass concentration or measured final volume.
6 Pyruvate
C3H3O3 87.054 g/mol
20 MILLIMOLAR CHEBI:15361
pyruvate
 Source-explicit 20 mM pyruvate added after autoclaving. Replaces the imported mass conversion; no molecular weight, stock strength or stock dose is inferred.

Preparation

  1. Adjust pH to 7.0.
  2. Boil under nitrogen gas and autoclave at 121°C for 15 min. Add 20 mM pyruvate after autoclaving.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:03.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 650
2026-02-03T11:19:05.825349 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 650
2026-03-13T06:19:39.137041Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:006227
2026-03-17T06:30:14.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:56.193401+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CENTENUM medium" to "centenum_medium" for consistent matching
2026-06-05T07:40:25.198040+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:45.471821+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-08T03:47:55.453935+00:00 codex CORRECTED_KOMODO650_752_762_AMOUNTS_AND_PREPARATION_STAGES Corrected source-explicit amounts and preparation stages. Exact excerpts accompany all changes; retained identity/history and linked records without propagating this formulation. No growth claim added.