Overview
- External ontology
- komodo.medium:655 (source link unavailable) — PYE medium
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: KOMODO ModelSEED | ID: 655 | DSMZ Medium: 655 (mediadive.medium:655) | Aerobic: No
Composition
flowchart LR
CultureMech_006233["pye_medium"]
ing0_K2HPO4["K2HPO4"]
CultureMech_006233 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006233 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_006233 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_006233 --> ing3_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing3_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing4_Na_pyruvate["Na-pyruvate"]
CultureMech_006233 --> ing4_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing4_Na_pyruvate -.-> CHEBI_50144
ing5_Yeast_extract["Yeast extract"]
CultureMech_006233 --> ing5_Yeast_extract
ing6__NH4_2SO4["(NH4)2SO4"]
CultureMech_006233 --> ing6__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing6__NH4_2SO4 -.-> CHEBI_62946
ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_006233 --> ing7_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_006233 --> ing8_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
ing9_H3BO3["H3BO3"]
CultureMech_006233 --> ing9_H3BO3
CHEBI_33118["CHEBI:33118"]
ing9_H3BO3 -.-> CHEBI_33118
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_006233 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_006233 --> ing11_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing11_CuCl2_x_2_H2O -.-> CHEBI_86318
ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_006233 --> ing12_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_006233 --> ing13_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.02 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
0.2 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 5 |
Na-pyruvate
C3H3O3.Na 110.044 g/mol
|
2.2 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 6 | Yeast extract | 4 G_PER_L | ||
| 7 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 8 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 9 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 10 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 13 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 14 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 655 |
| 2026-02-03T11:19:18.238171 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 655 |
| 2026-03-13T06:19:39.172537Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006233 |
| 2026-03-17T06:30:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:56.234166+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYE medium" to "pye_medium" for consistent matching |
| 2026-06-05T07:40:25.228345+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:45.484451+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |