Overview
- External ontology
- komodo.medium:659 (source link unavailable) — M17 medium (modified)
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.1, 'max': 7.2}
- Notes
- Source: KOMODO ModelSEED | ID: 659 | DSMZ Medium: 659 (mediadive.medium:659) | Aerobic: No Stock follow-up: quantities distinguish base-water inputs, stock strengths and batch doses; no measured final volume or new growth claim is inferred. Existing chemical mappings and variant links are retained, not independently validated.
Composition
flowchart LR
CultureMech_006236["m17_medium_modified"]
ing0_Casein_peptone["Casein peptone"]
CultureMech_006236 --> ing0_Casein_peptone
ing1_Soy_peptone["Soy peptone"]
CultureMech_006236 --> ing1_Soy_peptone
ing2_Meat_peptone["Meat peptone"]
CultureMech_006236 --> ing2_Meat_peptone
ing3_Yeast_extract["Yeast extract"]
CultureMech_006236 --> ing3_Yeast_extract
ing4_Ascorbic_acid["Ascorbic acid"]
CultureMech_006236 --> ing4_Ascorbic_acid
CHEBI_22652["CHEBI:22652"]
ing4_Ascorbic_acid -.-> CHEBI_22652
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006236 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_Na2___glycerolphosphate["Na2-ß-glycerolphosphate"]
CultureMech_006236 --> ing6_Na2___glycerolphosphate
CHEBI_132089["CHEBI:132089"]
ing6_Na2___glycerolphosphate -.-> CHEBI_132089
ing7_Lactose["Lactose"]
CultureMech_006236 --> ing7_Lactose
CHEBI_17716["CHEBI:17716"]
ing7_Lactose -.-> CHEBI_17716
ing8_Distilled_water["Distilled water"]
CultureMech_006236 --> ing8_Distilled_water
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casein peptone | 5 G_PER_L | Source batch input: 5 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a separately prepared base or stock concentration, or an independently measured final-volume concentration. | |
| 2 | Soy peptone | 5 G_PER_L | Source batch input: 5 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a separately prepared base or stock concentration, or an independently measured final-volume concentration. | |
| 3 | Meat peptone | 5 G_PER_L | Source batch input: 5 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a separately prepared base or stock concentration, or an independently measured final-volume concentration. | |
| 4 | Yeast extract | 2.5 G_PER_L | Source batch input: 2.5 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a separately prepared base or stock concentration, or an independently measured final-volume concentration. | |
| 5 | Ascorbic acid | 0.5 G_PER_L |
CHEBI:22652
ascorbic acid
|
Source batch input: 0.5 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a separately prepared base or stock concentration, or an independently measured final-volume concentration. |
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.25 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Source batch input: 0.25 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a separately prepared base or stock concentration, or an independently measured final-volume concentration. |
| 7 |
Na2-ß-glycerolphosphate
C3H7O6P.2Na 216.037 g/mol
|
9.5 G_PER_L |
CHEBI:132089
sodium glycerol 2-phosphate
|
Source batch input: 9.5 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a separately prepared base or stock concentration, or an independently measured final-volume concentration. |
| 8 |
Lactose
C12H22O11 342.297 g/mol
|
8 G_PER_L |
CHEBI:17716
lactose
|
Final-medium lactose target: 8.0 g/L, added after autoclaving as a filter-sterilized solution. Stock strength and addition volume are unspecified; 8 g/L is not the stock concentration. |
| 9 | Distilled water | 1 L |
mediadive.compound:4 (source link unavailable)
Distilled water
|
Distilled-water input to the base per recipe batch, not final medium volume or stock carrier volume. |
Preparation
- After autoclaving add a lactose solution (sterilized by filtration) up to a concentration of 8.0 g/l, and 1.2 ml 1M CaCl2 x 2 H2O. Adjust pH to 7.15 ± 0.05.
Solutions
CaCl2 x 2 H2O stock
Dose: 0.0012 L
| Ingredient | Concentration | Notes |
|---|---|---|
| CaCl2 x 2 H2O | 1 MOLAR | Source-explicit stock strength, not a final-medium concentration. The former flattened value is replaced at the stock stage; no molecular-weight or density conversion is inferred. |
1.2 ml stock addition per recipe batch, not per liter of an inferred final medium. Carrier amount and final-medium concentration are not inferred.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 659 |
| 2026-02-03T11:19:07.237747 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 659 |
| 2026-03-13T06:19:39.184395Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006236 |
| 2026-03-17T06:30:18.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:56.249216+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M17 medium (modified)" to "m17_medium_modified" for consistent matching |
| 2026-05-20T06:00:53.083891+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.1-7.2' -> {'min': 7.1, 'max': 7.2} |
| 2026-06-05T07:40:25.240646+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-08T04:59:45.451604+00:00 | codex | CORRECTED_DSMZ546_659_670_STOCK_STAGES | Restored source-explicit stock strengths/doses and base-water inputs; prior excerpts, history and mappings preserved. No strain-variant or growth curation is claimed. |