for_dsm_9466

Overview

External ontology

komodo.medium:670.3 (source link unavailable) — For DSM 9466

Applications

• Microbial cultivation

pH

3.5

Notes

pH buffer: H2SO4 | Source: KOMODO ModelSEED | ID: 670.3 | DSMZ Medium: 670 (mediadive.medium:670) | Aerobic: No Curated formulation: the explicitly named DSM strain instructions in BacDive 4303 archive version 2 (2017), not the current DSMZ 670 formulation. The archive agrees with the KOMODO pH profile, but that agreement does not prove which recipe version KOMODO originally imported. Current DSMZ differences are a separate inline variant. The BacDive biological record is DSM 2392; only its reproduced, explicitly named recipe instructions are used here, not its growth, temperature or taxonomic claims. Batch mass, unspecified percent basis, normality and final-volume gaps remain explicit. New ingredient labels are source labels, not independently resolved ontology mappings.

Composition

flowchart LR
    CultureMech_006262["for_dsm_9466"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_006262 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_006262 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_K2HPO4["K2HPO4"]
    CultureMech_006262 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_KCl["KCl"]
    CultureMech_006262 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_Distilled_water["Distilled water"]
    CultureMech_006262 --> ing4_Distilled_water
    ing5_H2SO4["H2SO4"]
    CultureMech_006262 --> ing5_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing5_H2SO4 -.-> CHEBI_26836
    ing6_Powdered_sulfur["Powdered sulfur"]
    CultureMech_006262 --> ing6_Powdered_sulfur
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_006262 --> ing7_Yeast_extract

Ingredients (8)

Preparation

1. For DSM 9466: Adjust pH of MS-medium to 3.5 with 1 N H2SO4 prior to autoclaving. Before use, add 5 g/l of sterile, powdered sulfur and 0.2 g/l of yeast extract (from sterile, autoclaved stock solution).

Target organisms

No target organisms are recorded.

Recipe variants

• Name

  DSM 9466 current DSMZ 670 source formulation

  Relationship

  DERIVED_FROM

  Description

  Separate recipe version; not applied to the asserted archived formulation.

  Modifications

  • Current DSMZ 670 base table uses 950 ml distilled-water input, not the archive's 1000 ml; this is not a stated final volume.
  • Current base preparation is autoclaving for 15 min at 121 C; incubate statically without shaking. These instructions are not imported into the historical formulation.
  • Omit FeSO4 x 7 H2O; add 5.00 g/L powdered sulfur sterilized by steaming in a boiling water bath for 3 hours on each of 3 successive days.
  • Current source: pH 3.0 using 1 N H2SO4, versus archived pH 3.5. Add 0.20 g/L yeast extract from a sterile stock; its strength, dose and sterilization method are unspecified.

  Evidence

  • Reference

    https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium670.pdf

    Supports

    SUPPORT

    Snippet

    For DSM 9466: Adjust pH to 3.0 with 1 N H2SO4 prior to autoclaving and omit FeSO4 x 7 H2O from the medium. Before use, add 5.00 g/l of powdered sulfur sterilized by steaming in a boiling water bath for 3 hours on each of 3 successive days and 0.20 g/l of yeast extract (from a sterile stock solution).

    Explanation

    variants[0].modifications (separate_source_version): Current-source changes apply only to this inline alternative; they do not repair the archive by substitution. Locator: PDF page 1. variants[0].description (separate_source_version): The same current-source base table and preparation qualify this alternative; final volume remains unstated. Locator: PDF page 1. Additional exact excerpt: 670: ACIDIFERROBACTER (MS) MEDIUM (NH4)2SO4 2.00 g MgSO4 x 7 H2O 0.25 g K2HPO4 0.10 g KCl 0.10 g FeSO4 x 7 H2O (40% w/v in 0.2 N H2SO4) 50.00 ml Distilled water 950.00 ml 1. Dissolve ingredients except ferrous sulfate and adjust pH to 2.2 with 4 N H2SO4. Sterilize medium by autoclaving for 15 min at 121°C. Before use, add FeSO4 x 7 H2O from a 40% (w/v) stock solution prepared with 0.2 N H2SO4 and filter-sterilized under 100% N2 gas atmosphere. 2. Note: Incubate statically without shaking.

Provenance