for_dsm_9468

Overview

External ontology

komodo.medium:670.5 (source link unavailable) — For DSM 9468

Applications

• Microbial cultivation

pH

1.6

Notes

Source: KOMODO ModelSEED | ID: 670.5 | DSMZ Medium: 670 (mediadive.medium:670) | Aerobic: No Curated formulation: the explicitly named DSM strain instructions in BacDive 4303 archive version 2 (2017), not the current DSMZ 670 formulation. The archive agrees with the KOMODO pH profile, but that agreement does not prove which recipe version KOMODO originally imported. Current DSMZ differences are a separate inline variant. The BacDive biological record is DSM 2392; only its reproduced, explicitly named recipe instructions are used here, not its growth, temperature or taxonomic claims. Batch mass, unspecified percent basis, normality and final-volume gaps remain explicit. New ingredient labels are source labels, not independently resolved ontology mappings.

Composition

flowchart LR
    CultureMech_006264["for_dsm_9468"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_006264 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_006264 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_K2HPO4["K2HPO4"]
    CultureMech_006264 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_KCl["KCl"]
    CultureMech_006264 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_Distilled_water["Distilled water"]
    CultureMech_006264 --> ing4_Distilled_water
    ing5_H2SO4["H2SO4"]
    CultureMech_006264 --> ing5_H2SO4

Ingredients (6)

Preparation

1. For DSM 9468: Adjust pH of MS-medium to 1.6 with 4 N H2SO4 prior to autoclaving. Before use, add 0.25 ml 40% (w/v) FeSO4 x 7 H2O solution (dissolved in 0.2 N H2SO4 and filter sterilized or autoclaved under N2) per 5 ml MS-medium.

Solutions

Target organisms

No target organisms are recorded.

Recipe variants

• Name

  DSM 9468 current DSMZ 670 source formulation

  Relationship

  DERIVED_FROM

  Description

  Separate recipe version; not applied to the asserted archived formulation.

  Modifications

  • Current DSMZ 670 base table uses 950 ml distilled-water input, not the archive's 1000 ml; this is not a stated final volume.
  • Current base preparation is autoclaving for 15 min at 121 C; incubate statically without shaking. These instructions are not imported into the historical formulation.
  • Current source: pH 1.6 with 4 N H2SO4 and 50 ml of 40% w/v FeSO4 x 7 H2O stock per recipe batch. Stock is made in 0.2 N H2SO4 and filter-sterilized under 100% N2; the old per-5-ml-base ratio and autoclaving alternative are not asserted for this version.

  Evidence

  • Reference

    https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium670.pdf

    Supports

    SUPPORT

    Snippet

    For DSM 9468: Adjust pH to 1.6 with 4 N H2SO4 prior to autoclaving.

    Explanation

    variants[0].modifications (separate_source_version): Current-source changes apply only to this inline alternative; they do not repair the archive by substitution. Locator: PDF page 1. variants[0].description (separate_source_version): The same current-source base table and preparation qualify this alternative; final volume remains unstated. Locator: PDF page 1. Additional exact excerpt: 670: ACIDIFERROBACTER (MS) MEDIUM (NH4)2SO4 2.00 g MgSO4 x 7 H2O 0.25 g K2HPO4 0.10 g KCl 0.10 g FeSO4 x 7 H2O (40% w/v in 0.2 N H2SO4) 50.00 ml Distilled water 950.00 ml 1. Dissolve ingredients except ferrous sulfate and adjust pH to 2.2 with 4 N H2SO4. Sterilize medium by autoclaving for 15 min at 121°C. Before use, add FeSO4 x 7 H2O from a 40% (w/v) stock solution prepared with 0.2 N H2SO4 and filter-sterilized under 100% N2 gas atmosphere. 2. Note: Incubate statically without shaking.

Provenance