Overview
- External ontology
- komodo.medium:670 (source link unavailable) — MS-MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 2.2
- Notes
- Source: KOMODO ModelSEED | ID: 670 | DSMZ Medium: 670 (mediadive.medium:670) | Aerobic: No Stock follow-up: quantities distinguish base-water inputs, stock strengths and batch doses; no measured final volume or new growth claim is inferred. Existing chemical mappings and variant links are retained, not independently validated. Note: Incubate statically without shaking. This correction covers the base formulation only; strain-specific alternatives require separate review. Four DSM 9463/9466/9467/9468 children now carry explicitly archived formulations and separate current-source alternatives. Their relationship is strain-specific, not pH-only. This does not re-curate the other two duplicate-source links.
Composition
flowchart LR
CultureMech_006265["ms_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_006265 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006265 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_K2HPO4["K2HPO4"]
CultureMech_006265 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_KCl["KCl"]
CultureMech_006265 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_Distilled_water["Distilled water"]
CultureMech_006265 --> ing4_Distilled_water
ing5_H2SO4["H2SO4"]
CultureMech_006265 --> ing5_H2SO4
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Source batch input: 2 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a separately prepared base or stock concentration, or an independently measured final-volume concentration. |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.25 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Source batch input: 0.25 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a separately prepared base or stock concentration, or an independently measured final-volume concentration. |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Source batch input: 0.1 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a separately prepared base or stock concentration, or an independently measured final-volume concentration. |
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.1 G_PER_L |
CHEBI:32588
potassium chloride
|
Source batch input: 0.1 g. Retained g/L is the provider-declared nominal main-recipe rate (volume field 1000 ml), not a separately prepared base or stock concentration, or an independently measured final-volume concentration. |
| 5 | Distilled water | 0.95 L |
mediadive.compound:4 (source link unavailable)
Distilled water
|
Distilled-water input to the base per recipe batch, not final medium volume or stock carrier volume. |
| 6 | H2SO4 | Use 4 N H2SO4 to adjust the base to pH 2.2 before autoclaving; addition amount is not quantified. Normality is retained as text, not converted to an unsupported concentration unit. This is not the 0.2 N iron-stock carrier. |
Preparation
- Dissolve ingredients except ferrous sulfate and adjust pH to 2.2 with 4 N H2SO4. Sterilize medium by autoclaving for 15 min at 121°C. Before use, add FeSO4 x 7 H2O from a 40% (w/v) stock solution prepared with 0.2 N H2SO4 and filter-sterilized under 100% N2 gas atmosphere.
Solutions
FeSO4 x 7 H2O stock
Dose: 0.05 L
| Ingredient | Concentration | Notes |
|---|---|---|
| FeSO4 x 7 H2O | 40 PERCENT_W_V | Source-explicit stock strength, not a final-medium concentration. The former flattened value is replaced at the stock stage; no molecular-weight or density conversion is inferred. |
| H2SO4 | Stock carrier is 0.2 N H2SO4; carrier amount is unspecified. Normality is retained as source text because no NORMAL unit exists in the concentration enum. This is distinct from the 4 N reagent used to adjust base pH. |
50 ml stock addition per recipe batch, not per liter of an inferred final medium. Carrier amount and final-medium concentration are not inferred.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 670 |
| 2026-02-03T11:18:55.665237 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 670 |
| 2026-03-13T06:19:39.354136Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006265 |
| 2026-03-17T06:29:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:56.453994+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MS-MEDIUM" to "ms_medium" for consistent matching |
| 2026-06-05T07:40:25.336224+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:45.519092+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-13T00:00:00-07:00 | repair_komodo_670_ms_ph_score10.py | RESOLVED_KOMODO_670_MS_PH_VARIANTS | Linked reviewed pH-specific KOMODO 670 strain variants. |
| 2026-10-08T04:59:45.451604+00:00 | codex | CORRECTED_DSMZ546_659_670_STOCK_STAGES | Restored source-explicit stock strengths/doses and base-water inputs; prior excerpts, history and mappings preserved. No strain-variant or growth curation is claimed. |
| 2026-10-08T05:41:55.293153+00:00 | codex | CURATED_KOMODO670_STRAIN_RECIPES_WITH_SEPARATE_SOURCE_VERSIONS | Explicit archived formulation curation with separate current-source alternatives; no new organism-growth association. Prior history is preserved, not endorsed. |