Overview
- External ontology
- komodo.medium:677 (source link unavailable) — P-2 medium
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.0, 'max': 7.2}
- Notes
- Source: KOMODO ModelSEED | ID: 677 | DSMZ Medium: 677 (mediadive.medium:677) | Aerobic: Yes
Composition
flowchart LR
CultureMech_006273["p_2_medium"]
ing0_K2HPO4["K2HPO4"]
CultureMech_006273 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_KH2PO4["KH2PO4"]
CultureMech_006273 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_006273 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_006273 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_006273 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Yeast_extract["Yeast extract"]
CultureMech_006273 --> ing5_Yeast_extract
ing6_Sodium_resazurin["Sodium resazurin"]
CultureMech_006273 --> ing6_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Sodium_resazurin -.-> CHEBI_8806
ing7_D_Glucose["D-Glucose"]
CultureMech_006273 --> ing7_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing7_D_Glucose -.-> CHEBI_17634
ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_006273 --> ing8_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_006273 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
2 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 | Yeast extract | 5 G_PER_L | ||
| 7 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 |
D-Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17634
D-glucose
|
|
| 9 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 677 |
| 2026-02-03T11:19:03.971595 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 677 |
| 2026-03-13T06:19:39.407068Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006273 |
| 2026-03-17T06:30:07.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:56.515885+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "P-2 medium" to "p_2_medium" for consistent matching |
| 2026-05-20T06:02:01.748221+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.0-7.2' -> {'min': 7.0, 'max': 7.2} |
| 2026-06-05T07:41:40.754575+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.842491+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |