for_s_buswellii_dsm_2612m (CultureMech:006276)

Overview

External ontology

komodo.medium:679.1 (source link unavailable) — For S. buswellii DSM 2612M

Applications

Microbial cultivation

pH

7.2

Notes

Archived DSMZ Medium 679 defines the S. buswellii DSM 2612M variant as DSMZ Medium 298 with butanediol omitted, 10 mL/L DSMZ Medium 503 Seven Vitamins Solution, 10 mM sodium crotonate, and sodium dithionite after inoculation; this record expands archived DSMZ Media 298, 320, 383, 503, and 679 into final per-liter components.

Composition

flowchart LR
    CultureMech_006276["for_s_buswellii_dsm_2612m"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_006276 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_006276 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_NaCl["NaCl"]
    CultureMech_006276 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_006276 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_KCl["KCl"]
    CultureMech_006276 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_006276 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_Resazurin["Resazurin"]
    CultureMech_006276 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_HCl["HCl"]
    CultureMech_006276 --> ing7_HCl
    CHEBI_17883["CHEBI:17883"]
    ing7_HCl -.-> CHEBI_17883
    ing8_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_006276 --> ing8_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing8_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing9_ZnCl2["ZnCl2"]
    CultureMech_006276 --> ing9_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing9_ZnCl2 -.-> CHEBI_49976
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_006276 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_H3BO3["H3BO3"]
    CultureMech_006276 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_006276 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_006276 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_006276 --> ing14_NiCl2_x_6_H2O
    CHEBI_53542["CHEBI:53542"]
    ing14_NiCl2_x_6_H2O -.-> CHEBI_53542
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_006276 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing16_NaHCO3["NaHCO3"]
    CultureMech_006276 --> ing16_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing16_NaHCO3 -.-> CHEBI_32139
    ing17_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_006276 --> ing17_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing17_Na2S_x_9_H2O -.-> CHEBI_76209
    ing18_sodium_crotonate["sodium crotonate"]
    CultureMech_006276 --> ing18_sodium_crotonate
    CHEBI_35899["CHEBI:35899"]
    ing18_sodium_crotonate -.-> CHEBI_35899
    ing19_Vitamin_B12["Vitamin B12"]
    CultureMech_006276 --> ing19_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing19_Vitamin_B12 -.-> CHEBI_176843
    ing20_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_006276 --> ing20_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing20_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing21_D____Biotin["D(+)-Biotin"]
    CultureMech_006276 --> ing21_D____Biotin
    CHEBI_15956["CHEBI:15956"]
    ing21_D____Biotin -.-> CHEBI_15956
    ing22_Nicotinic_acid["Nicotinic acid"]
    CultureMech_006276 --> ing22_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing22_Nicotinic_acid -.-> CHEBI_15940
    ing23_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_006276 --> ing23_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing23_Calcium_pantothenate -.-> CHEBI_31345
    ing24_Pyridoxine_HCl["Pyridoxine-HCl"]
    CultureMech_006276 --> ing24_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing24_Pyridoxine_HCl -.-> CHEBI_30961
    ing25_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_006276 --> ing25_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing25_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing26_Na2S2O4["Na2S2O4"]
    CultureMech_006276 --> ing26_Na2S2O4
    CHEBI_66870["CHEBI:66870"]
    ing26_Na2S2O4 -.-> CHEBI_66870
    ing27_CO2["CO2"]
    CultureMech_006276 --> ing27_CO2
    CHEBI_16526["CHEBI:16526"]
    ing27_CO2 -.-> CHEBI_16526
    ing28_N2["N2"]
    CultureMech_006276 --> ing28_N2
    CHEBI_17997["CHEBI:17997"]
    ing28_N2 -.-> CHEBI_17997
    ing29_Distilled_water["Distilled water"]
    CultureMech_006276 --> ing29_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing29_Distilled_water -.-> CHEBI_15377

Ingredients (30)

#	Ingredient	Concentration	Ontology	Notes
1	KH2PO4 H2O4P.K 136.084 g/mol	0.197824 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Archived DSMZ Medium 298 contributes 0.20 g KH2PO4 to a 1011 mL final formulation, yielding 0.197824 g/L.
2	NH4Cl Cl.H4N 53.492 g/mol	0.247280 G_PER_L	CHEBI:31206 ammonium chloride	Archived DSMZ Medium 298 contributes 0.25 g NH4Cl to a 1011 mL final formulation, yielding 0.247280 g/L.
3	NaCl Cl.Na 58.443 g/mol	0.989120 G_PER_L	CHEBI:26710 sodium chloride	Archived DSMZ Medium 298 contributes 1.00 g NaCl to a 1011 mL final formulation, yielding 0.989120 g/L.
4	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	0.395648 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	Archived DSMZ Medium 298 contributes 0.40 g MgCl2 x 6 H2O to a 1011 mL final formulation, yielding 0.395648 g/L.
5	KCl Cl.K 74.551 g/mol	0.494560 G_PER_L	CHEBI:32588 potassium chloride	Archived DSMZ Medium 298 contributes 0.50 g KCl to a 1011 mL final formulation, yielding 0.494560 g/L.
6	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.148368 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Archived DSMZ Medium 298 contributes 0.15 g CaCl2 x 2 H2O to a 1011 mL final formulation, yielding 0.148368 g/L.
7	Resazurin C12H7NO4 229.191 g/mol	0.000989 G_PER_L	CHEBI:8806 Resazurin	Archived DSMZ Medium 298 contributes 1.00 mg Resazurin to a 1011 mL final formulation, yielding 0.000989 g/L.
8	HCl HCl 36.461 g/mol	0.002473 G_PER_L	CHEBI:17883 hydrogen chloride	Archived DSMZ Medium 298 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1011 mL final formulation; the SL-10 stock contains 2.50 g/L HCl, yielding 0.002473 g/L.
9	FeCl2 x 4 H2O Cl2Fe.4H2O 198.811 g/mol	0.001484 G_PER_L	CHEBI:86249 iron dichloride tetrahydrate	Archived DSMZ Medium 298 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1011 mL final formulation; the SL-10 stock contains 1.50 g/L FeCl2 x 4 H2O, yielding 0.001484 g/L.
10	ZnCl2 2Cl.Zn 136.296 g/mol	0.0000692 G_PER_L	CHEBI:49976 zinc dichloride	Archived DSMZ Medium 298 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1011 mL final formulation; the SL-10 stock contains 0.070 g/L ZnCl2, yielding 0.0000692 g/L.
11	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.0000989 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	Archived DSMZ Medium 298 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1011 mL final formulation; the SL-10 stock contains 0.100 g/L MnCl2 x 4 H2O, yielding 0.0000989 g/L.
12	H3BO3 H3BO3 61.833 g/mol	0.00000593 G_PER_L	CHEBI:33118 boric acid	Archived DSMZ Medium 298 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1011 mL final formulation; the SL-10 stock contains 0.006 g/L H3BO3, yielding 0.00000593 g/L.
13	CoCl2 x 6 H2O 2Cl.Co.6H2O 237.929 g/mol	0.000188 G_PER_L	CHEBI:53503 cobalt chloride hexahydrate	Archived DSMZ Medium 298 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1011 mL final formulation; the SL-10 stock contains 0.190 g/L CoCl2 x 6 H2O, yielding 0.000188 g/L.
14	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	0.00000198 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	Archived DSMZ Medium 298 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1011 mL final formulation; the SL-10 stock contains 0.002 g/L CuCl2 x 2 H2O, yielding 0.00000198 g/L.
15	NiCl2 x 6 H2O 2Cl.6H2O.Ni 237.689 g/mol	0.0000237 G_PER_L	CHEBI:53542 nickel chloride hexahydrate	Archived DSMZ Medium 298 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1011 mL final formulation; the SL-10 stock contains 0.024 g/L NiCl2 x 6 H2O, yielding 0.0000237 g/L.
16	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.0000356 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Archived DSMZ Medium 298 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1011 mL final formulation; the SL-10 stock contains 0.036 g/L Na2MoO4 x 2 H2O, yielding 0.0000356 g/L.
17	NaHCO3 CHO3.Na 84.006 g/mol	2.472799 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Archived DSMZ Medium 298 contributes 2.50 g NaHCO3 to a 1011 mL final formulation, yielding 2.472799 g/L.
18	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.356083 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	Archived DSMZ Medium 298 contributes 0.36 g Na2S x 9 H2O to a 1011 mL final formulation, yielding 0.356083 g/L.
19	sodium crotonate C4H5O2 85.082 g/mol	10.000000 MILLIMOLAR	CHEBI:35899 crotonate	Archived DSMZ Medium 679 adds 10 mM sodium crotonate for S. buswellii DSM 2612M.
20	Vitamin B12	0.000989 G_PER_L	CHEBI:176843 vitamin B12	Archived DSMZ Medium 679 uses 10 mL/L of the Archived DSMZ Medium 503 Seven Vitamins Solution; the Seven Vitamins stock contains 0.100 g/L Vitamin B12, yielding 0.000989 g/L in the 1011 mL final formulation.
21	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	0.000791 G_PER_L	CHEBI:30753 4-aminobenzoic acid	Archived DSMZ Medium 679 uses 10 mL/L of the Archived DSMZ Medium 503 Seven Vitamins Solution; the Seven Vitamins stock contains 0.080 g/L p-Aminobenzoic acid, yielding 0.000791 g/L in the 1011 mL final formulation.
22	D(+)-Biotin C10H16N2O3S 244.316 g/mol	0.000198 G_PER_L	CHEBI:15956 biotin	Archived DSMZ Medium 679 uses 10 mL/L of the Archived DSMZ Medium 503 Seven Vitamins Solution; the Seven Vitamins stock contains 0.020 g/L D(+)-Biotin, yielding 0.000198 g/L in the 1011 mL final formulation.
23	Nicotinic acid C6H5NO2 123.111 g/mol	0.001978 G_PER_L	CHEBI:15940 nicotinic acid	Archived DSMZ Medium 679 uses 10 mL/L of the Archived DSMZ Medium 503 Seven Vitamins Solution; the Seven Vitamins stock contains 0.200 g/L Nicotinic acid, yielding 0.001978 g/L in the 1011 mL final formulation.
24	Calcium pantothenate 2C9H16NO5.Ca 476.536 g/mol	0.000989 G_PER_L	CHEBI:31345 Calcium pantothenate	Archived DSMZ Medium 679 uses 10 mL/L of the Archived DSMZ Medium 503 Seven Vitamins Solution; the Seven Vitamins stock contains 0.100 g/L Calcium pantothenate, yielding 0.000989 g/L in the 1011 mL final formulation.
25	Pyridoxine-HCl C8H11NO3.HCl 205.641 g/mol	0.002967 G_PER_L	CHEBI:30961 pyridoxine hydrochloride	Archived DSMZ Medium 679 uses 10 mL/L of the Archived DSMZ Medium 503 Seven Vitamins Solution; the Seven Vitamins stock contains 0.300 g/L Pyridoxine hydrochloride, yielding 0.002967 g/L in the 1011 mL final formulation.
26	Thiamine-HCl x 2 H2O C12H18N4OS.2Cl.2H2O 373.306 g/mol	0.001978 G_PER_L	CHEBI:132751 Thiamine-HCl x 2 H2O	Archived DSMZ Medium 679 uses 10 mL/L of the Archived DSMZ Medium 503 Seven Vitamins Solution; the Seven Vitamins stock contains 0.200 g/L Thiamine-HCl x 2 H2O, yielding 0.001978 g/L in the 1011 mL final formulation.
27	Na2S2O4 2Na.O4S2 174.11 g/mol	0.010-0.020 G_PER_L	CHEBI:66870 sodium dithionite	Archived DSMZ Medium 679 adds sodium dithionite as reductant after inoculation; Archived DSMZ Medium 383 sodium dithionite instruction states that adding 10-20 mg/L sodium dithionite can stimulate growth.
28	CO2 CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Archived DSMZ Medium 298 prepares the medium under 80% N2 and 20% CO2.
29	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Archived DSMZ Medium 298 prepares the medium under 80% N2 and 20% CO2.
30	Distilled water H2O 18.015 g/mol	1000.000 ML_PER_L	CHEBI:15377 water	Archived DSMZ Medium 298 lists 1000 mL distilled water before adding the SL-10 trace solution and later anaerobic stock solutions.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:03.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 679.1
2026-03-13T06:19:39.430786Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:006276
2026-03-17T19:45:56.543840+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "For S. buswellii DSM 2612M" to "for_s_buswellii_dsm_2612m" for consistent matching
2026-08-06T08:58:32.438565+00:00	curate_flag_empty_composition.py	FLAGGED_INCOMPLETE_COMPOSITION	Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175).
2026-09-10T00:00:00-07:00	repair_komodo_679_score35.py	RESOLVED_KOMODO_679_SCORE35	Archived DSMZ Medium 679 defines the S. buswellii DSM 2612M variant as DSMZ Medium 298 with butanediol omitted, 10 mL/L DSMZ Medium 503 Seven Vitamins Solution, 10 mM sodium crotonate, and sodium dithionite after inoculation; this record expands archived DSMZ Media 298, 320, 383, 503, and 679 into final per-liter components.