desulfotomaculum_sp_medium_ii (CultureMech:006282)

Overview

External ontology

komodo.medium:682 (source link unavailable) — DESULFOTOMACULUM SP. MEDIUM II

Applications

Microbial cultivation

pH range

{'min': 7.1, 'max': 7.4}

Notes

Archived DSMZ Medium 682 defines DESULFOTOMACULUM SP. MEDIUM II as DSMZ Medium 194 with sodium sulfate lowered to 0.7 g/L, DSMZ Medium 503 Seven Vitamins Solution, and 2 mM 3,4,5-trimethoxybenzoate replacing propionate; KOMODO Medium 682 shows the DSMZ Medium 141 vitamin solution is retained and the DSMZ Medium 503 stock is added, so this record expands archived DSMZ Media 193, 194, 320, 141, and 503 into final per-liter components.

Composition

flowchart LR
    CultureMech_006282["desulfotomaculum_sp_medium_ii"]
    ing0_Na2SO4["Na2SO4"]
    CultureMech_006282 --> ing0_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing0_Na2SO4 -.-> CHEBI_32149
    ing1_KH2PO4["KH2PO4"]
    CultureMech_006282 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_006282 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaCl["NaCl"]
    CultureMech_006282 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_006282 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_KCl["KCl"]
    CultureMech_006282 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_006282 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_Resazurin["Resazurin"]
    CultureMech_006282 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_HCl["HCl"]
    CultureMech_006282 --> ing8_HCl
    CHEBI_17883["CHEBI:17883"]
    ing8_HCl -.-> CHEBI_17883
    ing9_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_006282 --> ing9_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing9_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing10_ZnCl2["ZnCl2"]
    CultureMech_006282 --> ing10_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing10_ZnCl2 -.-> CHEBI_49976
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_006282 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_H3BO3["H3BO3"]
    CultureMech_006282 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_006282 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_006282 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_006282 --> ing15_NiCl2_x_6_H2O
    CHEBI_53542["CHEBI:53542"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_53542
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_006282 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_NaHCO3["NaHCO3"]
    CultureMech_006282 --> ing17_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing17_NaHCO3 -.-> CHEBI_32139
    ing18_3_4_5_trimethoxybenzoate["3,4,5-trimethoxybenzoate"]
    CultureMech_006282 --> ing18_3_4_5_trimethoxybenzoate
    CHEBI_454991["CHEBI:454991"]
    ing18_3_4_5_trimethoxybenzoate -.-> CHEBI_454991
    ing19_Biotin["Biotin"]
    CultureMech_006282 --> ing19_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing19_Biotin -.-> CHEBI_15956
    ing20_Folic_acid["Folic acid"]
    CultureMech_006282 --> ing20_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing20_Folic_acid -.-> CHEBI_27470
    ing21_D____Biotin["D(+)-Biotin"]
    CultureMech_006282 --> ing21_D____Biotin
    ing21_D____Biotin -.-> CHEBI_15956
    ing22_Pyridoxine_HCl["Pyridoxine-HCl"]
    CultureMech_006282 --> ing22_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing22_Pyridoxine_HCl -.-> CHEBI_30961
    ing23_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_006282 --> ing23_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing23_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing24_Riboflavin["Riboflavin"]
    CultureMech_006282 --> ing24_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing24_Riboflavin -.-> CHEBI_17015
    ing25_Nicotinic_acid["Nicotinic acid"]
    CultureMech_006282 --> ing25_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing25_Nicotinic_acid -.-> CHEBI_15940
    ing26_D_Ca_pantothenate["D-Ca-pantothenate"]
    CultureMech_006282 --> ing26_D_Ca_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing26_D_Ca_pantothenate -.-> CHEBI_31345
    ing27_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_006282 --> ing27_Calcium_pantothenate
    ing27_Calcium_pantothenate -.-> CHEBI_31345
    ing28_Vitamin_B12["Vitamin B12"]
    CultureMech_006282 --> ing28_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing28_Vitamin_B12 -.-> CHEBI_176843
    ing29_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_006282 --> ing29_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing29_p_Aminobenzoic_acid -.-> CHEBI_30753
    CultureMech_006282 --> more["...5 more"]

Ingredients (35)

#	Ingredient	Concentration	Ontology	Notes
1	Na2SO4 2Na.O4S 142.043 g/mol	0.700000 G_PER_L	CHEBI:32149 sodium sulfate	Archived DSMZ Medium 682 lowers the sodium sulfate amount to 0.7 g/L.
2	KH2PO4 H2O4P.K 136.084 g/mol	0.199601 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Archived DSMZ Medium 193 contributes 0.20 g KH2PO4 to a 1002 mL final formulation, yielding 0.199601 g/L.
3	NH4Cl Cl.H4N 53.492 g/mol	0.299401 G_PER_L	CHEBI:31206 ammonium chloride	Archived DSMZ Medium 193 contributes 0.30 g NH4Cl to a 1002 mL final formulation, yielding 0.299401 g/L.
4	NaCl Cl.Na 58.443 g/mol	1.000000 G_PER_L	CHEBI:26710 sodium chloride	Archived DSMZ Medium 194 lowers the DSMZ Medium 193 NaCl amount to 1.0 g/L.
5	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	0.400000 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	Archived DSMZ Medium 194 lowers the DSMZ Medium 193 MgCl2 x 6 H2O amount to 0.4 g/L.
6	KCl Cl.K 74.551 g/mol	0.499002 G_PER_L	CHEBI:32588 potassium chloride	Archived DSMZ Medium 193 contributes 0.50 g KCl to a 1002 mL final formulation, yielding 0.499002 g/L.
7	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.149701 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Archived DSMZ Medium 193 contributes 0.15 g CaCl2 x 2 H2O to a 1002 mL final formulation, yielding 0.149701 g/L.
8	Resazurin C12H7NO4 229.191 g/mol	0.000998 G_PER_L	CHEBI:8806 Resazurin	Archived DSMZ Medium 193 contributes 1.00 mg Resazurin to a 1002 mL final formulation, yielding 0.000998 g/L.
9	HCl HCl 36.461 g/mol	0.002495 G_PER_L	CHEBI:17883 hydrogen chloride	Archived DSMZ Medium 193 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1002 mL final formulation; the SL-10 stock contains 2.50 g/L HCl, yielding 0.002495 g/L.
10	FeCl2 x 4 H2O Cl2Fe.4H2O 198.811 g/mol	0.001497 G_PER_L	CHEBI:86249 iron dichloride tetrahydrate	Archived DSMZ Medium 193 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1002 mL final formulation; the SL-10 stock contains 1.50 g/L FeCl2 x 4 H2O, yielding 0.001497 g/L.
11	ZnCl2 2Cl.Zn 136.296 g/mol	0.0000699 G_PER_L	CHEBI:49976 zinc dichloride	Archived DSMZ Medium 193 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1002 mL final formulation; the SL-10 stock contains 0.070 g/L ZnCl2, yielding 0.0000699 g/L.
12	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.0000998 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	Archived DSMZ Medium 193 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1002 mL final formulation; the SL-10 stock contains 0.100 g/L MnCl2 x 4 H2O, yielding 0.0000998 g/L.
13	H3BO3 H3BO3 61.833 g/mol	0.00000599 G_PER_L	CHEBI:33118 boric acid	Archived DSMZ Medium 193 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1002 mL final formulation; the SL-10 stock contains 0.006 g/L H3BO3, yielding 0.00000599 g/L.
14	CoCl2 x 6 H2O 2Cl.Co.6H2O 237.929 g/mol	0.000190 G_PER_L	CHEBI:53503 cobalt chloride hexahydrate	Archived DSMZ Medium 193 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1002 mL final formulation; the SL-10 stock contains 0.190 g/L CoCl2 x 6 H2O, yielding 0.000190 g/L.
15	CuCl2 x 2 H2O Cl2Cu.2H2O 170.482 g/mol	0.00000200 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	Archived DSMZ Medium 193 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1002 mL final formulation; the SL-10 stock contains 0.002 g/L CuCl2 x 2 H2O, yielding 0.00000200 g/L.
16	NiCl2 x 6 H2O 2Cl.6H2O.Ni 237.689 g/mol	0.0000240 G_PER_L	CHEBI:53542 nickel chloride hexahydrate	Archived DSMZ Medium 193 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1002 mL final formulation; the SL-10 stock contains 0.024 g/L NiCl2 x 6 H2O, yielding 0.0000240 g/L.
17	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.0000359 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Archived DSMZ Medium 193 adds 1 mL of the Archived DSMZ Medium 320 SL-10 trace element solution to a 1002 mL final formulation; the SL-10 stock contains 0.036 g/L Na2MoO4 x 2 H2O, yielding 0.0000359 g/L.
18	NaHCO3 CHO3.Na 84.006 g/mol	4.990020 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Archived DSMZ Medium 193 contributes 5.00 g NaHCO3 to a 1002 mL final formulation, yielding 4.990020 g/L.
19	3,4,5-trimethoxybenzoate C10H12O5 212.201 g/mol	2.000 MILLIMOLAR	CHEBI:454991 3,4,5-trimethoxybenzoic acid	Archived DSMZ Medium 682 replaces sodium propionate with 2 mM 3,4,5-trimethoxybenzoate.
20	Biotin C10H16N2O3S 244.316 g/mol	0.0000200 G_PER_L	CHEBI:15956 biotin	Archived DSMZ Medium 193 adds 10 mL of the Archived DSMZ Medium 141 vitamin solution to a 1002 mL final formulation; the vitamin stock contains 0.002 g/L Biotin, yielding 0.0000200 g/L.
21	Folic acid C19H19N7O6 441.404 g/mol	0.0000200 G_PER_L	CHEBI:27470 folic acid	Archived DSMZ Medium 193 adds 10 mL of the Archived DSMZ Medium 141 vitamin solution to a 1002 mL final formulation; the vitamin stock contains 0.002 g/L Folic acid, yielding 0.0000200 g/L.
22	D(+)-Biotin C10H16N2O3S 244.316 g/mol	0.0000200 G_PER_L	CHEBI:15956 biotin	Archived DSMZ Medium 682 uses the Archived DSMZ Medium 503 Seven Vitamins Solution; KOMODO Medium 682 expands it at 1 mL/L, and the stock contains 0.020 g/L D(+)-Biotin, yielding 0.0000200 g/L in the 1002 mL final formulation.
23	Pyridoxine-HCl C8H11NO3.HCl 205.641 g/mol	0.000399 G_PER_L	CHEBI:30961 pyridoxine hydrochloride	Archived DSMZ Medium 141 vitamin solution and Archived DSMZ Medium 503 Seven Vitamins Solution both contribute Pyridoxine-HCl; their scaled contributions of 0.0000998 and 0.000299 g/L yield 0.000399 g/L in the 1002 mL final formulation.
24	Thiamine-HCl x 2 H2O C12H18N4OS.2Cl.2H2O 373.306 g/mol	0.000250 G_PER_L	CHEBI:132751 Thiamine-HCl x 2 H2O	Archived DSMZ Medium 141 vitamin solution and Archived DSMZ Medium 503 Seven Vitamins Solution both contribute Thiamine-HCl x 2 H2O; their scaled contributions of 0.0000499 and 0.000200 g/L yield 0.000250 g/L in the 1002 mL final formulation.
25	Riboflavin C17H20N4O6 376.369 g/mol	0.0000499 G_PER_L	CHEBI:17015 riboflavin	Archived DSMZ Medium 193 adds 10 mL of the Archived DSMZ Medium 141 vitamin solution to a 1002 mL final formulation; the vitamin stock contains 0.005 g/L Riboflavin, yielding 0.0000499 g/L.
26	Nicotinic acid C6H5NO2 123.111 g/mol	0.000250 G_PER_L	CHEBI:15940 nicotinic acid	Archived DSMZ Medium 141 vitamin solution and Archived DSMZ Medium 503 Seven Vitamins Solution both contribute Nicotinic acid; their scaled contributions of 0.0000499 and 0.000200 g/L yield 0.000250 g/L in the 1002 mL final formulation.
27	D-Ca-pantothenate 2C9H16NO5.Ca 476.536 g/mol	0.0000499 G_PER_L	CHEBI:31345 Calcium pantothenate	Archived DSMZ Medium 193 adds 10 mL of the Archived DSMZ Medium 141 vitamin solution to a 1002 mL final formulation; the vitamin stock contains 0.005 g/L D-Ca-pantothenate, yielding 0.0000499 g/L.
28	Calcium pantothenate 2C9H16NO5.Ca 476.536 g/mol	0.0000998 G_PER_L	CHEBI:31345 Calcium pantothenate	Archived DSMZ Medium 682 uses the Archived DSMZ Medium 503 Seven Vitamins Solution; KOMODO Medium 682 expands it at 1 mL/L, and the stock contains 0.100 g/L Calcium pantothenate, yielding 0.0000998 g/L in the 1002 mL final formulation.
29	Vitamin B12	0.000101 G_PER_L	CHEBI:176843 vitamin B12	Archived DSMZ Medium 141 vitamin solution and Archived DSMZ Medium 503 Seven Vitamins Solution both contribute Vitamin B12; their scaled contributions of 0.000000998 and 0.0000998 g/L yield 0.000101 g/L in the 1002 mL final formulation.
30	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	0.000130 G_PER_L	CHEBI:30753 4-aminobenzoic acid	Archived DSMZ Medium 141 vitamin solution and Archived DSMZ Medium 503 Seven Vitamins Solution both contribute p-Aminobenzoic acid; their scaled contributions of 0.0000499 and 0.0000798 g/L yield 0.000130 g/L in the 1002 mL final formulation.
31	Lipoic acid C8H14O2S2 206.332 g/mol	0.0000499 G_PER_L	CHEBI:16494 lipoic acid	Archived DSMZ Medium 193 adds 10 mL of the Archived DSMZ Medium 141 vitamin solution to a 1002 mL final formulation; the vitamin stock contains 0.005 g/L Lipoic acid, yielding 0.0000499 g/L.
32	Na2S x 9 H2O 9H2O.2Na.S 240.182 g/mol	0.399202 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	Archived DSMZ Medium 193 contributes 0.40 g Na2S x 9 H2O to a 1002 mL final formulation, yielding 0.399202 g/L.
33	CO2 CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Archived DSMZ Medium 193 prepares the medium under 80% N2 and 20% CO2.
34	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Archived DSMZ Medium 193 prepares the medium under 80% N2 and 20% CO2.
35	Distilled water H2O 18.015 g/mol	990.000 ML_PER_L	CHEBI:15377 water	Archived DSMZ Medium 193 lists 990 mL direct distilled water across solutions A, C, D, and F before stock additions and before Archived DSMZ Medium 682 replaces the propionate with 3,4,5-trimethoxybenzoate.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:03.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 682
2026-03-13T06:19:39.443451Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:006282
2026-03-17T19:45:56.558803+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "DESULFOTOMACULUM SP. MEDIUM II" to "desulfotomaculum_sp_medium_ii" for consistent matching
2026-08-06T08:58:32.420006+00:00	curate_flag_empty_composition.py	FLAGGED_INCOMPLETE_COMPOSITION	Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175).
2026-09-10T00:00:00-07:00	repair_komodo_682_score35.py	RESOLVED_KOMODO_682_SCORE35	Archived DSMZ Medium 682 defines DESULFOTOMACULUM SP. MEDIUM II as DSMZ Medium 194 with sodium sulfate lowered to 0.7 g/L, DSMZ Medium 503 Seven Vitamins Solution, and 2 mM 3,4,5-trimethoxybenzoate replacing propionate; KOMODO Medium 682 shows the DSMZ Medium 141 vitamin solution is retained and the DSMZ Medium 503 stock is added, so this record expands archived DSMZ Media 193, 194, 320, 141, and 503 into final per-liter components.