Overview
- External ontology
- komodo.medium:708 (source link unavailable) — DESULFORHABDUS AMNIGENUS MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.2, 'max': 7.6}
- Notes
- Source: KOMODO ModelSEED | ID: 708 | DSMZ Medium: 708 (mediadive.medium:708) | Aerobic: Yes
Composition
flowchart LR
CultureMech_006340["desulforhabdus_amnigenus_medium"]
ing0_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_006340 --> ing0_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing0_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing1_KH2PO4["KH2PO4"]
CultureMech_006340 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_006340 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_006340 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_006340 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_Na2SO4["Na2SO4"]
CultureMech_006340 --> ing5_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing5_Na2SO4 -.-> CHEBI_32149
ing6_NaCl["NaCl"]
CultureMech_006340 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_Sodium_resazurin["Sodium resazurin"]
CultureMech_006340 --> ing7_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Sodium_resazurin -.-> CHEBI_8806
ing8_Na2CO3["Na2CO3"]
CultureMech_006340 --> ing8_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing8_Na2CO3 -.-> CHEBI_29377
ing9_Na_propionate["Na-propionate"]
CultureMech_006340 --> ing9_Na_propionate
CHEBI_132106["CHEBI:132106"]
ing9_Na_propionate -.-> CHEBI_132106
ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_006340 --> ing10_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing10_Na2S_x_9_H2O -.-> CHEBI_76209
ing11_HCl["HCl"]
CultureMech_006340 --> ing11_HCl
CHEBI_17883["CHEBI:17883"]
ing11_HCl -.-> CHEBI_17883
ing12_ZnCl2["ZnCl2"]
CultureMech_006340 --> ing12_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing12_ZnCl2 -.-> CHEBI_49976
ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_006340 --> ing13_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
ing14_H3BO3["H3BO3"]
CultureMech_006340 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_006340 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_006340 --> ing16_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_006340 --> ing17_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_006340 --> ing18_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing19_NaOH["NaOH"]
CultureMech_006340 --> ing19_NaOH
CHEBI_32145["CHEBI:32145"]
ing19_NaOH -.-> CHEBI_32145
ing20_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_006340 --> ing20_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing20_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_006340 --> ing21_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing22_Biotin["Biotin"]
CultureMech_006340 --> ing22_Biotin
CHEBI_15956["CHEBI:15956"]
ing22_Biotin -.-> CHEBI_15956
ing23_Folic_acid["Folic acid"]
CultureMech_006340 --> ing23_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing23_Folic_acid -.-> CHEBI_27470
ing24_Thiamine_HCl["Thiamine HCl"]
CultureMech_006340 --> ing24_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing24_Thiamine_HCl -.-> CHEBI_49105
ing25_Riboflavin["Riboflavin"]
CultureMech_006340 --> ing25_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing25_Riboflavin -.-> CHEBI_17015
ing26_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_006340 --> ing26_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing26_Calcium_D_____pantothenate -.-> CHEBI_31345
ing27__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_006340 --> ing27__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing27__DL__alpha_Lipoic_acid -.-> CHEBI_16494
ing28_D_____biotin["D-(+)-biotin"]
CultureMech_006340 --> ing28_D_____biotin
ing28_D_____biotin -.-> CHEBI_15956
ing29_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_006340 --> ing29_Calcium_pantothenate
ing29_Calcium_pantothenate -.-> CHEBI_31345
Ingredients (30)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
0.527888 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.408367 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.298805 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.109562 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.0996016 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 6 |
Na2SO4
2Na.O4S 142.043 g/mol
|
2.78884 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
0.298805 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000498008 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
Na2CO3
CO3.2Na 105.988 g/mol
|
1.49402 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 10 |
Na-propionate
C3H5O2.Na 96.061 g/mol
|
1.99203 G_PER_L |
CHEBI:132106
sodium propionate
|
|
| 11 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.498008 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 12 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 13 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 14 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 17 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 18 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 19 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 20 |
NaOH
HO.Na 39.997 g/mol
|
0.5 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 21 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 22 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 23 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 24 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 25 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 26 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 27 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 28 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 29 |
D-(+)-biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 30 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Solutions
Trace element solution SL-10
| Ingredient | Concentration | Notes |
|---|---|---|
| FeCl2 x 4 H2O | 1.5 G_PER_L |
Seven vitamins solution
| Ingredient | Concentration | Notes |
|---|---|---|
| Thiamine-HCl x 2 H2O | 0.2 G_PER_L | |
| Nicotinic acid | 0.2 G_PER_L | [Merged 2 duplicates: 0.05, 0.2] |
| p-Aminobenzoic acid | 0.08 G_PER_L | [Merged 2 duplicates: 0.05, 0.08] |
| Pyridoxine hydrochloride | 0.3 G_PER_L | [Merged 2 duplicates: 0.1, 0.3] |
| Vitamin B12 | 0.1 G_PER_L | [Merged 2 duplicates: 0.001, 0.1] |
Wolin's vitamin solution (10x)
| Ingredient | Concentration | Notes |
|---|---|---|
| Nicotinic acid | 0.05 G_PER_L | [Merged 2 duplicates: 0.05, 0.2] |
| p-Aminobenzoic acid | 0.05 G_PER_L | [Merged 2 duplicates: 0.05, 0.08] |
| Pyridoxine hydrochloride | 0.1 G_PER_L | [Merged 2 duplicates: 0.1, 0.3] |
| Vitamin B12 | 0.001 G_PER_L | [Merged 2 duplicates: 0.001, 0.1] |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 708 |
| 2026-02-03T11:18:57.170787 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 708 |
| 2026-02-06T05:10:16.432268Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:08:44.718717Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:39.870741Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006340 |
| 2026-03-17T06:29:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:57.041463+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFORHABDUS AMNIGENUS MEDIUM" to "desulforhabdus_amnigenus_medium" for consistent matching |
| 2026-05-20T06:01:14.307669+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.2-7.6' -> {'min': 7.2, 'max': 7.6} |
| 2026-06-05T07:40:57.159487+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 34 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:55.788356+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-15T00:17:28.810242+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 6 stock-strength component(s) out of ingredients into 3 solution(s): 1 -> 'Trace element solution SL-10' @ 1 ml/l; 1 -> 'Seven vitamins solution' @ 1 ml/l; 0 -> "Wolin's vitamin solution (10x)" @ 1 ml/l. 4 component(s) were SUMMED across stocks by the flattening and are restored to each stock's own value, verified by exact sum: nicotinic acid, p-aminobenzoic acid, pyridoxine hydrochloride, vitamin b12. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |
| 2026-08-15T05:34:52.044642+00:00 | promote_komodo_volume_candidates.py | SHARPENED_VOLUME_COUNTEREVIDENCE | This record reproduces MediaDive medium 708 ('DESULFORHABDUS AMNIGENA MEDIUM') across 36 shared compounds at 89% identical values. That is short of the exact agreement required to assert the volume, so it stays a candidate (#262). |
| 2026-08-15T05:57:24.147240+00:00 | promote_komodo_volume_candidates.py | SHARPENED_VOLUME_COUNTEREVIDENCE | This record reproduces MediaDive medium 708 ('DESULFORHABDUS AMNIGENA MEDIUM') across 36 shared compounds at 89% identical values, 11 of them from that medium's main solution. That is short of the exact agreement required to assert the volume, so it stays a candidate (#262). |