thiobacillus_ferrooxidans_medium_with_thiosulfate (CultureMech:006362)

Overview

External ontology

komodo.medium:71 (source link unavailable) — THIOBACILLUS FERROOXIDANS MEDIUM WITH THIOSULFATE

Applications

Microbial cultivation

pH range

{'min': 4.4, 'max': 4.7}

Notes

Source: KOMODO ModelSEED | ID: 71 | DSMZ Medium: 71 (mediadive.medium:71) | Aerobic: No

Composition

flowchart LR
    CultureMech_006362["thiobacillus_ferrooxidans_medium_with_thiosulfate"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_006362 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_006362 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2__NH4_2SO4["(NH4)2SO4"]
    CultureMech_006362 --> ing2__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing2__NH4_2SO4 -.-> CHEBI_62946
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_006362 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_006362 --> ing4_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing4_Na2S2O3_x_5_H2O -.-> CHEBI_32150

Ingredients (5)

#	Ingredient	Concentration	Ontology
1	KH2PO4 H2O4P.K 136.084 g/mol	3 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate
2	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.5 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
3	(NH4)2SO4 2H4N.O4S 132.141 g/mol	3 G_PER_L	CHEBI:62946 ammonium sulfate
4	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.25 G_PER_L	CHEBI:86158 calcium chloride dihydrate
5	Na2S2O3 x 5 H2O 5H2O.2Na.O3S2 248.186 g/mol	5 G_PER_L	CHEBI:32150 sodium thiosulfate pentahydrate

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:03.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 71
2026-02-03T11:19:10.698385	dsmz-resolver-v1.0	Enriched with DSMZ composition	Copied ingredients from DSMZ Medium 71
2026-03-13T06:19:40.112777Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:006362
2026-03-17T06:30:28.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:57.319211+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "THIOBACILLUS FERROOXIDANS MEDIUM WITH THIOSULFATE" to "thiobacillus_ferrooxidans_medium_with_thiosulfate" for consistent matching
2026-05-20T06:02:09.078225+00:00	migrate_ph_range.py	MIGRATED_PH_RANGE	ph_range '4.4-4.7' -> {'min': 4.4, 'max': 4.7}
2026-06-05T07:41:51.225344+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:13.418903+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-13T00:00:00-07:00	repair_komodo_71_acidithiobacillus_score10.py	RESOLVED_KOMODO_71_ACIDITHIOBACILLUS_TOPOLOGY	KOMODO Medium 71 has the same 5-ingredient signature as DSMZ Medium 71.
2026-10-07T20:30:39.950647+00:00	codex	CURATED_KOMODO_CONCENTRATION_SOURCE_EVIDENCE	Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. KOMODO accession 71 is mapped to the same DSMZ accession by https://komodo.modelseed.org/servlet/KomodoTomcatServerSideUtilitiesModelSeed?MediaList; mapping response SHA256=d8259d98e6dbffdf6961a1ab92bc86f3567d5d96de352bfa079edb2a91e9580a. The mapping supports identity only, not ingredient quantities or variants.