clostridium_longisporum_medium (CultureMech:006392)

Overview

External ontology

komodo.medium:741 (source link unavailable) — CLOSTRIDIUM LONGISPORUM medium

Applications

Microbial cultivation

pH

6.8

Notes

Archived DSMZ Medium 741 defines CLOSTRIDIUM LONGISPORUM MEDIUM as Reinforced Clostridial Medium (Oxoid CM 149) prepared under 100% CO2 with final pH 6.8; KOMODO Medium 741 expands the RCM base into final per-liter components.

Composition

flowchart LR
    CultureMech_006392["clostridium_longisporum_medium"]
    ing0_Agar["Agar"]
    CultureMech_006392 --> ing0_Agar
    CHEBI_2509["CHEBI:2509"]
    ing0_Agar -.-> CHEBI_2509
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_006392 --> ing1_Yeast_extract
    ing2_Glucose["Glucose"]
    CultureMech_006392 --> ing2_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing2_Glucose -.-> CHEBI_17634
    ing3_CO2["CO2"]
    CultureMech_006392 --> ing3_CO2
    CHEBI_16526["CHEBI:16526"]
    ing3_CO2 -.-> CHEBI_16526
    ing4_Sodium_acetate["Sodium acetate"]
    CultureMech_006392 --> ing4_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing4_Sodium_acetate -.-> CHEBI_32954
    ing5_Cysteine_hydrochloride["Cysteine hydrochloride"]
    CultureMech_006392 --> ing5_Cysteine_hydrochloride
    CHEBI_91247["CHEBI:91247"]
    ing5_Cysteine_hydrochloride -.-> CHEBI_91247
    ing6_Lab_Lemco_powder["Lab-Lemco powder"]
    CultureMech_006392 --> ing6_Lab_Lemco_powder
    ing7_Sodium_chloride["Sodium chloride"]
    CultureMech_006392 --> ing7_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing7_Sodium_chloride -.-> CHEBI_26710
    ing8_Soluble_starch["Soluble starch"]
    CultureMech_006392 --> ing8_Soluble_starch
    CHEBI_28017["CHEBI:28017"]
    ing8_Soluble_starch -.-> CHEBI_28017
    ing9_Peptone["Peptone"]
    CultureMech_006392 --> ing9_Peptone

Ingredients (10)

#	Ingredient	Concentration	Ontology	Notes
1	Agar	15.000000 G_PER_L	CHEBI:2509 agar	KOMODO Medium 741 expands the RCM base and lists 15.00 g/L agar.
2	Yeast extract	3.000000 G_PER_L	FOODON:03315426 yeast extract	KOMODO Medium 741 expands the RCM base and lists 3.00 g/L yeast extract.
3	Glucose C6H12O6 180.156 g/mol	5.000000 G_PER_L	CHEBI:17634 D-glucose	KOMODO Medium 741 expands the RCM base and lists 5.00 g/L glucose.
4	CO2 CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Archived DSMZ Medium 741 prepares the medium under 100% CO2.
5	Sodium acetate C2H3O2.Na 82.034 g/mol	3.000000 G_PER_L	CHEBI:32954 sodium acetate	KOMODO Medium 741 expands the RCM base and lists 3.00 g/L sodium acetate.
6	Cysteine hydrochloride C3H7NO2S.HCl 157.622 g/mol	0.500000 G_PER_L	CHEBI:91247 L-cysteine hydrochloride	KOMODO Medium 741 expands the RCM base and lists 0.50 g/L cysteine hydrochloride.
7	Lab-Lemco powder	10.000000 G_PER_L	FOODON:03302088 beef extract	KOMODO Medium 741 expands the RCM base and lists 10.00 g/L Lab-Lemco powder.
8	Sodium chloride Cl.Na 58.443 g/mol	5.000000 G_PER_L	CHEBI:26710 sodium chloride	KOMODO Medium 741 expands the RCM base and lists 5.00 g/L sodium chloride.
9	Soluble starch	1.000000 G_PER_L	CHEBI:28017 starch	KOMODO Medium 741 expands the RCM base and lists 1.00 g/L soluble starch.
10	Peptone	10.000000 G_PER_L	MICRO:0000178 (source link unavailable) peptone	KOMODO Medium 741 expands the RCM base and lists 10.00 g/L peptone.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:03.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 741
2026-03-13T06:19:40.389873Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:006392
2026-03-17T19:45:57.682067+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "CLOSTRIDIUM LONGISPORUM medium" to "clostridium_longisporum_medium" for consistent matching
2026-08-06T08:58:32.408881+00:00	curate_flag_empty_composition.py	FLAGGED_INCOMPLETE_COMPOSITION	Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175).
2026-09-10T00:00:00-07:00	repair_komodo_741_score30.py	RESOLVED_KOMODO_741_SCORE30	Archived DSMZ Medium 741 defines CLOSTRIDIUM LONGISPORUM MEDIUM as Reinforced Clostridial Medium (Oxoid CM 149) prepared under 100% CO2 with final pH 6.8; KOMODO Medium 741 expands the RCM base into final per-liter components.
2026-09-22T00:03:26.686152+00:00	repair_rejected_peptone_groundings.py	CORRECTED_REJECTED_PEPTONE_GROUNDING	CultureMech #484. /ingredients/9/term/id: Peptone FOODON:03302071 -> MICRO:0000178 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved.