sc_medium

Overview

External ontology

komodo.medium:751 (source link unavailable) — SC medium

Applications

• Microbial cultivation

pH

6.6

Notes

Source: KOMODO ModelSEED | ID: 751 | DSMZ Medium: 751 (mediadive.medium:751) | Aerobic: No Superseded historical provenance (not a current formulation claim): Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. The LB constituent expansion is withdrawn: the exact DSMZ formulation specifies bovine serum albumin instead. Source batch, stock and receiving-medium scopes are separate; chemical groundings and growth claims are not recertified.

Composition

flowchart LR
    CultureMech_006403["sc_medium"]
    ing0_Corn_meal_agar["Corn meal agar"]
    CultureMech_006403 --> ing0_Corn_meal_agar
    CHEBI_2509["CHEBI:2509"]
    ing0_Corn_meal_agar -.-> CHEBI_2509
    ing1_Soy_peptone["Soy peptone"]
    CultureMech_006403 --> ing1_Soy_peptone
    ing2_K2HPO4["K2HPO4"]
    CultureMech_006403 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_KH2PO4["KH2PO4"]
    CultureMech_006403 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_006403 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_Distilled_water["Distilled water"]
    CultureMech_006403 --> ing5_Distilled_water

Ingredients (6)

Preparation

1. Adjust the basal medium to pH 6.6 before autoclaving.
2. Autoclave the basal medium, including the hemin input; the source gives no fixed cycle temperature or duration.
3. Cool to 50 C, then add the separately filter-sterilized albumin, glucose and cysteine stocks.

Solutions

Target organisms

No target organisms are recorded.

Recipe variants

• Name

  Liquid SC medium

  Relationship

  PHYSICAL_STATE_VARIANT

  Description

  DSMZ 751 liquid formulation with altered phosphate batch inputs; not a sequential change to the agar base recipe.

  Modifications

  • Omit corn meal agar.
  • Use KH2PO4 1.5 g per batch instead of 1 g.
  • Use K2HPO4 0.5 g per batch instead of 1 g.

  Purpose

  Liquid medium

  Evidence

  • Reference

    https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium751.pdf

    Supports

    SUPPORT

    Snippet

    For liquid medium, omit corn meal agar and change the phosphate concentration to KH2PO4, 1.5 g and K2HPO4, 0.5 g.

    Explanation

    variants[0].modifications: All three modifications belong together. Grams per batch are not assumed grams per final liter. PDF page 1; layout extraction and page image inspected. No final-medium volume, percentage basis, density or chemical grounding is inferred. Locator=original recipe text; accessed=2026-10-08T21:53:37.797126+00:00; capture SHA256=4e923b20851eebb337f189949dd4ff7bc75ca8eb0309ae2ca9e4f7651b6673d3.

Provenance