Overview
- External ontology
- komodo.medium:762 (source link unavailable) — HALOMONAS DESIDERATA MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 9.5, 'max': 10.0}
- Notes
- Source: KOMODO ModelSEED | ID: 762 | DSMZ Medium: 762 (mediadive.medium:762) | Aerobic: No Concentration follow-up: water volumes denote batch inputs, not final volume. Historical source-duplicate links and import annotations are preserved but not revalidated by this correction; linked records may still carry flattened quantities. Preparation stages follow the cited source.
Composition
flowchart LR
CultureMech_006417["halomonas_desiderata_medium"]
ing0_Glucose["Glucose"]
CultureMech_006417 --> ing0_Glucose
CHEBI_17234["CHEBI:17234"]
ing0_Glucose -.-> CHEBI_17234
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_006417 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_KH2PO4["KH2PO4"]
CultureMech_006417 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_KNO3["KNO3"]
CultureMech_006417 --> ing3_KNO3
CHEBI_63043["CHEBI:63043"]
ing3_KNO3 -.-> CHEBI_63043
ing4_Distilled_water["Distilled water"]
CultureMech_006417 --> ing4_Distilled_water
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
Source batch input: 5 g. Retained g/L is the provider's nominal main-recipe rate (volume field 1000 ml), not a measured final-medium concentration or a stock concentration. |
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Source batch input: 0.2 g. Retained g/L is the provider's nominal main-recipe rate (volume field 1000 ml), not a measured final-medium concentration or a stock concentration. |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Source batch input: 1 g. Retained g/L is the provider's nominal main-recipe rate (volume field 1000 ml), not a measured final-medium concentration or a stock concentration. |
| 4 |
KNO3
K.NO3 101.102 g/mol
|
2 G_PER_L |
CHEBI:63043
potassium nitrate
|
Source batch input: 2 g. Retained g/L is the provider's nominal main-recipe rate (volume field 1000 ml), not a measured final-medium concentration or a stock concentration. |
| 5 | Distilled water | 0.9 L |
mediadive.compound:4 (source link unavailable)
Distilled water
|
900 ml distilled-water input for the base, excluding the separate carbonate preparation; not a final volume. |
Preparation
- Prepare the medium without Na2CO3 and NaHCO3 in 900 ml distilled water and autoclave. Prepare the Na2CO3 and NaHCO3 in 100 ml water, sterilise separately and add to the cooled medium. Final pH 9.5-10.0.
Solutions
Separately sterilized carbonate preparation
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2CO3 | Source batch input: 5.4 g Na2CO3 in the separately sterilized carbonate preparation. The flattened g/L rate is withdrawn at this stock stage; 100 ml water is not final stock volume. No stock or final-medium concentration is calculated. | |
| NaHCO3 | Source batch input: 4.2 g NaHCO3 in the separately sterilized carbonate preparation. The flattened g/L rate is withdrawn at this stock stage; 100 ml water is not final stock volume. No stock or final-medium concentration is calculated. | |
| Distilled water | 0.1 L | 100 ml water input for the carbonate preparation, not a final stock volume or stock dose. |
100 ml water is preparation input, not final stock volume or numeric stock addition dose. Stock strength and final-medium concentrations are unasserted. Separate sterilization method is not specified.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 762 |
| 2026-02-03T11:19:05.836660 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 762 |
| 2026-03-13T06:19:40.570460Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006417 |
| 2026-03-17T06:30:14.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:57.907640+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOMONAS DESIDERATA MEDIUM" to "halomonas_desiderata_medium" for consistent matching |
| 2026-05-20T06:00:53.667538+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '9.5-10.0' -> {'min': 9.5, 'max': 10.0} |
| 2026-06-05T07:40:26.158644+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.310766+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:45.784301+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-10-08T03:47:55.453935+00:00 | codex | CORRECTED_KOMODO650_752_762_AMOUNTS_AND_PREPARATION_STAGES | Corrected source-explicit amounts and preparation stages. Exact excerpts accompany all changes; retained identity/history and linked records without propagating this formulation. No growth claim added. |