r_cw_medium (CultureMech:006432)

Overview

External ontology

komodo.medium:775 (source link unavailable) — R - CW medium

Applications

Microbial cultivation

pH

5.5

Notes

Source: KOMODO ModelSEED | ID: 775 | DSMZ Medium: 775 (mediadive.medium:775) | Aerobic: No

Composition

flowchart LR
    CultureMech_006432["r_cw_medium"]
    ing0_Trypticase["Trypticase"]
    CultureMech_006432 --> ing0_Trypticase
    ing1_Tryptone["Tryptone"]
    CultureMech_006432 --> ing1_Tryptone
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_006432 --> ing2_Yeast_extract
    ing3_KH2PO4["KH2PO4"]
    CultureMech_006432 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_Na_acetate["Na-acetate"]
    CultureMech_006432 --> ing4_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing4_Na_acetate -.-> CHEBI_32954
    ing5__NH4_2_citrate["(NH4)2 citrate"]
    CultureMech_006432 --> ing5__NH4_2_citrate
    CHEBI_63076["CHEBI:63076"]
    ing5__NH4_2_citrate -.-> CHEBI_63076
    ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_006432 --> ing6_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing7_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_006432 --> ing7_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing7_MnSO4_x_H2O -.-> CHEBI_86364
    ing8_Tween_80["Tween 80"]
    CultureMech_006432 --> ing8_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing8_Tween_80 -.-> CHEBI_53426
    ing9_Cheese_whey["Cheese whey"]
    CultureMech_006432 --> ing9_Cheese_whey

Ingredients (10)

#	Ingredient	Concentration	Ontology
1	Trypticase	5 G_PER_L	MICRO:0000175 (source link unavailable) Trypticase peptone
2	Tryptone	5 G_PER_L
3	Yeast extract	5 G_PER_L
4	KH2PO4 H2O4P.K 136.084 g/mol	5 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate
5	Na-acetate C2H3O2.Na 82.034 g/mol	5 G_PER_L	CHEBI:32954 sodium acetate
6	(NH4)2 citrate C6H6O7.2H4N 226.185 g/mol	2 G_PER_L	CHEBI:63076 diammonium citrate
7	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.5 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
8	MnSO4 x H2O H2O.Mn.O4S 169.016 g/mol	0.2 G_PER_L	CHEBI:86364 manganese(II) sulfate monohydrate
9	Tween 80 (C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol	1 G_PER_L	CHEBI:53426 polysorbate 80
10	Cheese whey	1000 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:03.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 775
2026-02-03T11:19:04.705964	dsmz-resolver-v1.0	Enriched with DSMZ composition	Copied ingredients from DSMZ Medium 775
2026-03-13T06:19:40.697106Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:006432
2026-03-17T06:30:10.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:58.054870+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "R - CW medium" to "r_cw_medium" for consistent matching
2026-06-05T07:40:26.273556+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:45.829325+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-22T00:03:26.686152+00:00	repair_rejected_peptone_groundings.py	CORRECTED_REJECTED_PEPTONE_GROUNDING	CultureMech #484. /ingredients/0/term/id: Trypticase CHEBI:78018 -> MICRO:0000175 (replace_term). Reviewed identities: https://github.com/CultureBotAI/MediaIngredientMech/tree/24562493919391df4468a4285b7c997960cc8868. Publication resolver: MIM label index 9f09e4fb97fb0e6cbbd6f25baca40b36512adb88. MediaDive source IDs and concentrations preserved.
2026-10-08T03:14:00.696534+00:00	codex	CURATED_KOMODO_CONCENTRATION_SOURCE_EVIDENCE	Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. KOMODO accession 775 is mapped to the same DSMZ accession by https://komodo.modelseed.org/servlet/KomodoTomcatServerSideUtilitiesModelSeed?MediaList; mapping response SHA256=d8259d98e6dbffdf6961a1ab92bc86f3567d5d96de352bfa079edb2a91e9580a. The mapping supports identity only, not ingredient quantities or variants.