Overview
- External ontology
- komodo.medium:78_22519 (source link unavailable) — MEDIUM 78 MODIFIED FOR DSM 22519
- Applications
-
- Microbial cultivation
- Notes
- Source: KOMODO ModelSEED | ID: 78_22519 | DSMZ Medium: 78 (mediadive.medium:78) | Aerobic: No
Composition
flowchart LR
CultureMech_006455["medium_78_modified_for_dsm_22519"]
ing0_L_Histidine["L-Histidine"]
CultureMech_006455 --> ing0_L_Histidine
CHEBI_15971["CHEBI:15971"]
ing0_L_Histidine -.-> CHEBI_15971
ing1_L_Serine["L-Serine"]
CultureMech_006455 --> ing1_L_Serine
CHEBI_17115["CHEBI:17115"]
ing1_L_Serine -.-> CHEBI_17115
ing2_L_Glutamine["L-Glutamine"]
CultureMech_006455 --> ing2_L_Glutamine
CHEBI_18050["CHEBI:18050"]
ing2_L_Glutamine -.-> CHEBI_18050
ing3_Vitamin_B12["Vitamin B12"]
CultureMech_006455 --> ing3_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing3_Vitamin_B12 -.-> CHEBI_176843
ing4_Pantothenic_acid["Pantothenic acid"]
CultureMech_006455 --> ing4_Pantothenic_acid
CHEBI_7916["CHEBI:7916"]
ing4_Pantothenic_acid -.-> CHEBI_7916
ing5_Riboflavin["Riboflavin"]
CultureMech_006455 --> ing5_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing5_Riboflavin -.-> CHEBI_17015
ing6_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
CultureMech_006455 --> ing6_Pyridoxamine_hydrochloride
CHEBI_131531["CHEBI:131531"]
ing6_Pyridoxamine_hydrochloride -.-> CHEBI_131531
ing7_Biotin["Biotin"]
CultureMech_006455 --> ing7_Biotin
CHEBI_15956["CHEBI:15956"]
ing7_Biotin -.-> CHEBI_15956
ing8_Folic_acid["Folic acid"]
CultureMech_006455 --> ing8_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing8_Folic_acid -.-> CHEBI_27470
ing9_Nicotinic_acid["Nicotinic acid"]
CultureMech_006455 --> ing9_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing9_Nicotinic_acid -.-> CHEBI_15940
ing10_Nicotine_amide["Nicotine amide"]
CultureMech_006455 --> ing10_Nicotine_amide
CHEBI_17154["CHEBI:17154"]
ing10_Nicotine_amide -.-> CHEBI_17154
ing11_alpha_lipoic_acid["alpha-lipoic acid"]
CultureMech_006455 --> ing11_alpha_lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing11_alpha_lipoic_acid -.-> CHEBI_16494
ing12_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_006455 --> ing12_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing12_p_Aminobenzoic_acid -.-> CHEBI_30753
ing13_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
CultureMech_006455 --> ing13_Thiamine_HCl_x_2_H2O
CHEBI_132751["CHEBI:132751"]
ing13_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
ing14_Ground_beef["Ground beef"]
CultureMech_006455 --> ing14_Ground_beef
ing15_NaOH["NaOH"]
CultureMech_006455 --> ing15_NaOH
CHEBI_32145["CHEBI:32145"]
ing15_NaOH -.-> CHEBI_32145
ing16_Casitone["Casitone"]
CultureMech_006455 --> ing16_Casitone
ing17_Yeast_extract["Yeast extract"]
CultureMech_006455 --> ing17_Yeast_extract
ing18_K2HPO4["K2HPO4"]
CultureMech_006455 --> ing18_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing18_K2HPO4 -.-> CHEBI_131527
ing19_Resazurin["Resazurin"]
CultureMech_006455 --> ing19_Resazurin
CHEBI_8806["CHEBI:8806"]
ing19_Resazurin -.-> CHEBI_8806
ing20_L_Cysteine_HCl["L-Cysteine HCl"]
CultureMech_006455 --> ing20_L_Cysteine_HCl
CHEBI_91247["CHEBI:91247"]
ing20_L_Cysteine_HCl -.-> CHEBI_91247
ing21_Agar["Agar"]
CultureMech_006455 --> ing21_Agar
CHEBI_2509["CHEBI:2509"]
ing21_Agar -.-> CHEBI_2509
ing22_Haemin["Haemin"]
CultureMech_006455 --> ing22_Haemin
CHEBI_50385["CHEBI:50385"]
ing22_Haemin -.-> CHEBI_50385
ing23_Vitamin_K3["Vitamin K3"]
CultureMech_006455 --> ing23_Vitamin_K3
CHEBI_28869["CHEBI:28869"]
ing23_Vitamin_K3 -.-> CHEBI_28869
ing24_Ethanol["Ethanol"]
CultureMech_006455 --> ing24_Ethanol
CHEBI_16236["CHEBI:16236"]
ing24_Ethanol -.-> CHEBI_16236
ing25_Vitamin_K1["Vitamin K1"]
CultureMech_006455 --> ing25_Vitamin_K1
CHEBI_18067["CHEBI:18067"]
ing25_Vitamin_K1 -.-> CHEBI_18067
Ingredients (26)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
L-Histidine
C6H9N3O2 155.157 g/mol
|
12 G_PER_L |
CHEBI:15971
L-histidine
|
|
| 2 |
L-Serine
C3H7NO3 105.093 g/mol
|
10 G_PER_L |
CHEBI:17115
L-serine
|
|
| 3 |
L-Glutamine
C5H10N2O3 146.146 g/mol
|
14 G_PER_L |
CHEBI:18050
L-glutamine
|
|
| 4 | Vitamin B12 | 0.05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 5 |
Pantothenic acid
C9H17NO5 219.237 g/mol
|
0.05 G_PER_L |
CHEBI:7916
pantothenic acid
|
|
| 6 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 7 |
Pyridoxamine hydrochloride
C8H12N2O2.HCl 204.657 g/mol
|
0.01 G_PER_L |
CHEBI:131531
pyridoxamine hydrochloride
|
|
| 8 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 9 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 10 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.025 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 11 |
Nicotine amide
C6H6N2O 122.127 g/mol
|
0.025 G_PER_L |
CHEBI:17154
nicotinamide
|
|
| 12 |
alpha-lipoic acid
C8H14O2S2 206.332 g/mol
|
0.05 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 13 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 14 |
Thiamine-HCl x 2 H2O
C12H18N4OS.2Cl.2H2O 373.306 g/mol
|
0.05 G_PER_L |
CHEBI:132751
thiamine hydrochloride dihydrate
|
|
| 15 | Ground beef | 487.805 G_PER_L | ||
| 16 |
NaOH
HO.Na 39.997 g/mol
|
26.0 G_PER_L |
CHEBI:32145
sodium hydroxide
|
[Merged 2 duplicates: 25.0, 1.0] |
| 17 | Casitone | 29.2683 G_PER_L | ||
| 18 | Yeast extract | 4.87805 G_PER_L | ||
| 19 |
K2HPO4
HO4P.2K 174.174 g/mol
|
4.87805 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 20 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.00097561 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 21 |
L-Cysteine HCl
C3H7NO2S.HCl 157.622 g/mol
|
0.487805 G_PER_L |
CHEBI:91247
L-cysteine hydrochloride
|
|
| 22 | Agar | 14.6341 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 23 |
Haemin
C34H32ClFeN4O4 651.952 g/mol
|
0.5 G_PER_L |
CHEBI:50385
hemin
|
|
| 24 |
Vitamin K3
C11H8O2 172.183 g/mol
|
0.05 G_PER_L |
CHEBI:28869
menadione
|
|
| 25 |
Ethanol
C2H6O 46.069 g/mol
|
959.5 G_PER_L |
CHEBI:16236
ethanol
|
[Merged 2 duplicates: 9.5, 950.0] |
| 26 |
Vitamin K1
C31H46O2 450.707 g/mol
|
0.1 G_PER_L |
CHEBI:18067
phylloquinone
|
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 78_22519 |
| 2026-02-03T11:19:19.644109 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 78 |
| 2026-03-13T03:09:44.113201Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:40.926880Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006455 |
| 2026-03-17T06:30:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:58.318507+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEDIUM 78 MODIFIED FOR DSM 22519" to "medium_78_modified_for_dsm_22519" for consistent matching |
| 2026-06-05T07:41:27.081696+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:05.409570+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |