acetobacterium_dehalogenans_medium (CultureMech:006488)

Overview

External ontology

komodo.medium:787 (source link unavailable) — ACETOBACTERIUM DEHALOGENANS medium

Applications

Microbial cultivation

pH

7.4

Notes

Archived DSMZ Medium 787 defines ACETOBACTERIUM DEHALOGENANS MEDIUM as DSMZ Medium 135 prepared without fructose, sodium sulfide, or bicarbonate in 900 mL water under 80:20 N2/CO2, with NaHCO3, Cysteine-HCl x H2O, and Na-syringate added to each 9 mL aliquot from sterile anaerobic stocks; this record expands the Medium 135 trace and vitamin stocks into final per-liter components after the 10.32 mL final aliquot volume.

Composition

flowchart LR
    CultureMech_006488["acetobacterium_dehalogenans_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_006488 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_KH2PO4["KH2PO4"]
    CultureMech_006488 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_K2HPO4["K2HPO4"]
    CultureMech_006488 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_006488 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_006488 --> ing4_Yeast_extract
    ing5_Resazurin["Resazurin"]
    CultureMech_006488 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_006488 --> ing6_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing6_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing7_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_006488 --> ing7_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing7_MnSO4_x_H2O -.-> CHEBI_86364
    ing8_NaCl["NaCl"]
    CultureMech_006488 --> ing8_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing8_NaCl -.-> CHEBI_26710
    ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_006488 --> ing9_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing10_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_006488 --> ing10_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing10_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing11_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_006488 --> ing11_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing11_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_006488 --> ing12_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing13_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_006488 --> ing13_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing13_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing14_KAl_SO4_2_x_12_H2O["KAl(SO4)2 x 12 H2O"]
    CultureMech_006488 --> ing14_KAl_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing14_KAl_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing15_H3BO3["H3BO3"]
    CultureMech_006488 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_006488 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_006488 --> ing17_NiCl2_x_6_H2O
    CHEBI_53542["CHEBI:53542"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_53542
    ing18_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_006488 --> ing18_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing18_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing19_KOH["KOH"]
    CultureMech_006488 --> ing19_KOH
    CHEBI_32035["CHEBI:32035"]
    ing19_KOH -.-> CHEBI_32035
    ing20_Biotin["Biotin"]
    CultureMech_006488 --> ing20_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing20_Biotin -.-> CHEBI_15956
    ing21_Folic_acid["Folic acid"]
    CultureMech_006488 --> ing21_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing21_Folic_acid -.-> CHEBI_27470
    ing22_Pyridoxine_HCl["Pyridoxine-HCl"]
    CultureMech_006488 --> ing22_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing22_Pyridoxine_HCl -.-> CHEBI_30961
    ing23_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_006488 --> ing23_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing23_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751
    ing24_Riboflavin["Riboflavin"]
    CultureMech_006488 --> ing24_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing24_Riboflavin -.-> CHEBI_17015
    ing25_Nicotinic_acid["Nicotinic acid"]
    CultureMech_006488 --> ing25_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing25_Nicotinic_acid -.-> CHEBI_15940
    ing26_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_006488 --> ing26_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing26_Calcium_pantothenate -.-> CHEBI_31345
    ing27_Vitamin_B12["Vitamin B12"]
    CultureMech_006488 --> ing27_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing27_Vitamin_B12 -.-> CHEBI_176843
    ing28_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_006488 --> ing28_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing28_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing29__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
    CultureMech_006488 --> ing29__DL__alpha_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing29__DL__alpha_Lipoic_acid -.-> CHEBI_16494
    CultureMech_006488 --> more["...6 more"]

Ingredients (36)

#	Ingredient	Concentration	Ontology	Notes
1	NH4Cl Cl.H4N 53.492 g/mol	0.927759 G_PER_L	CHEBI:31206 ammonium chloride	Archived DSMZ Medium 787 uses Archived DSMZ Medium 135 without fructose, bicarbonate, or sulfide and lists 1.00 g NH4Cl; after 9 mL base medium receives 1.32 mL of stock additions this yields 0.927759 g/L.
2	KH2PO4 H2O4P.K 136.084 g/mol	0.306160 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Archived DSMZ Medium 787 uses Archived DSMZ Medium 135 without fructose, bicarbonate, or sulfide and lists 0.33 g KH2PO4; after 9 mL base medium receives 1.32 mL of stock additions this yields 0.306160 g/L.
3	K2HPO4 HO4P.2K 174.174 g/mol	0.417491 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	Archived DSMZ Medium 787 uses Archived DSMZ Medium 135 without fructose, bicarbonate, or sulfide and lists 0.45 g K2HPO4; after 9 mL base medium receives 1.32 mL of stock additions this yields 0.417491 g/L.
4	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	0.145101 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate	Archived DSMZ Medium 787 uses Archived DSMZ Medium 135 containing 0.10 g MgSO4 x 7 H2O plus 20 mL of Archived DSMZ Medium 141 trace elements containing 3.00 g/L MgSO4 x 7 H2O; after 9 mL base medium receives 1.32 mL of stock additions this yields 0.145101 g/L.
5	Yeast extract	1.855517 G_PER_L	FOODON:03315426 yeast extract	Archived DSMZ Medium 787 uses Archived DSMZ Medium 135 without fructose, bicarbonate, or sulfide and lists 2.00 g Yeast extract; after 9 mL base medium receives 1.32 mL of stock additions this yields 1.855517 g/L.
6	Resazurin C12H7NO4 229.191 g/mol	0.000928 G_PER_L	CHEBI:8806 Resazurin	Archived DSMZ Medium 787 uses Archived DSMZ Medium 135 without fructose, bicarbonate, or sulfide and lists 1.00 mg Resazurin; after 9 mL base medium receives 1.32 mL of stock additions this yields 0.000928 g/L.
7	Nitrilotriacetic acid C6H9NO6 191.139 g/mol	0.026163 G_PER_L	CHEBI:44557 nitrilotriacetic acid	Archived DSMZ Medium 787 uses 20 mL of Archived DSMZ Medium 141 trace elements per Archived DSMZ Medium 135; the trace stock contains 1.50 g/L Nitrilotriacetic acid, yielding 0.026163 g/L after the DSMZ 787 stock additions.
8	MnSO4 x H2O H2O.Mn.O4S 169.016 g/mol	0.008721 G_PER_L	CHEBI:86364 manganese(II) sulfate monohydrate	Archived DSMZ Medium 787 uses 20 mL of Archived DSMZ Medium 141 trace elements per Archived DSMZ Medium 135; the trace stock contains 0.50 g/L MnSO4 x H2O, yielding 0.008721 g/L after the DSMZ 787 stock additions.
9	NaCl Cl.Na 58.443 g/mol	0.017442 G_PER_L	CHEBI:26710 sodium chloride	Archived DSMZ Medium 787 uses 20 mL of Archived DSMZ Medium 141 trace elements per Archived DSMZ Medium 135; the trace stock contains 1.00 g/L NaCl, yielding 0.017442 g/L after the DSMZ 787 stock additions.
10	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	0.001744 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate	Archived DSMZ Medium 787 uses 20 mL of Archived DSMZ Medium 141 trace elements per Archived DSMZ Medium 135; the trace stock contains 0.10 g/L FeSO4 x 7 H2O, yielding 0.001744 g/L after the DSMZ 787 stock additions.
11	CoSO4 x 7 H2O Co.7H2O.O4S 281.101 g/mol	0.003140 G_PER_L	CHEBI:91244 cobalt(2+) sulfate heptahydrate	Archived DSMZ Medium 787 uses 20 mL of Archived DSMZ Medium 141 trace elements per Archived DSMZ Medium 135; the trace stock contains 0.18 g/L CoSO4 x 7 H2O, yielding 0.003140 g/L after the DSMZ 787 stock additions.
12	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.001744 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Archived DSMZ Medium 787 uses 20 mL of Archived DSMZ Medium 141 trace elements per Archived DSMZ Medium 135; the trace stock contains 0.10 g/L CaCl2 x 2 H2O, yielding 0.001744 g/L after the DSMZ 787 stock additions.
13	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	0.003140 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate	Archived DSMZ Medium 787 uses 20 mL of Archived DSMZ Medium 141 trace elements per Archived DSMZ Medium 135; the trace stock contains 0.18 g/L ZnSO4 x 7 H2O, yielding 0.003140 g/L after the DSMZ 787 stock additions.
14	CuSO4 x 5 H2O Cu.5H2O.O4S 249.684 g/mol	0.000174 G_PER_L	CHEBI:31440 copper(II) sulfate pentahydrate	Archived DSMZ Medium 787 uses 20 mL of Archived DSMZ Medium 141 trace elements per Archived DSMZ Medium 135; the trace stock contains 0.01 g/L CuSO4 x 5 H2O, yielding 0.000174 g/L after the DSMZ 787 stock additions.
15	KAl(SO4)2 x 12 H2O Al.12H2O.K.2O4S 474.386 g/mol	0.000349 G_PER_L	CHEBI:86465 potassium aluminium sulfate dodecahydrate	Archived DSMZ Medium 787 uses 20 mL of Archived DSMZ Medium 141 trace elements per Archived DSMZ Medium 135; the trace stock contains 0.02 g/L KAl(SO4)2 x 12 H2O, yielding 0.000349 g/L after the DSMZ 787 stock additions.
16	H3BO3 H3BO3 61.833 g/mol	0.000174 G_PER_L	CHEBI:33118 boric acid	Archived DSMZ Medium 787 uses 20 mL of Archived DSMZ Medium 141 trace elements per Archived DSMZ Medium 135; the trace stock contains 0.01 g/L H3BO3, yielding 0.000174 g/L after the DSMZ 787 stock additions.
17	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.000174 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Archived DSMZ Medium 787 uses 20 mL of Archived DSMZ Medium 141 trace elements per Archived DSMZ Medium 135; the trace stock contains 0.01 g/L Na2MoO4 x 2 H2O, yielding 0.000174 g/L after the DSMZ 787 stock additions.
18	NiCl2 x 6 H2O 2Cl.6H2O.Ni 237.689 g/mol	0.000523 G_PER_L	CHEBI:53542 nickel chloride hexahydrate	Archived DSMZ Medium 787 uses 20 mL of Archived DSMZ Medium 141 trace elements per Archived DSMZ Medium 135; the trace stock contains 0.03 g/L NiCl2 x 6 H2O, yielding 0.000523 g/L after the DSMZ 787 stock additions.
19	Na2SeO3 x 5 H2O 5H2O.2Na.O3Se 263.012 g/mol	0.000005233 G_PER_L	CHEBI:131361 disodium selenite pentahydrate	Archived DSMZ Medium 787 uses 20 mL of Archived DSMZ Medium 141 trace elements per Archived DSMZ Medium 135; the trace stock contains 0.30 mg/L Na2SeO3 x 5 H2O, yielding 0.000005233 g/L after the DSMZ 787 stock additions.
20	KOH HO.K 56.105 g/mol	variable VARIABLE	CHEBI:32035 potassium hydroxide	Archived DSMZ Medium 141 adjusts the trace element solution with KOH; Archived DSMZ Medium 787 uses 20 mL of that trace solution via Medium 135.
21	Biotin C10H16N2O3S 244.316 g/mol	0.000034884 G_PER_L	CHEBI:15956 Biotin	Archived DSMZ Medium 787 uses 20 mL of Archived DSMZ Medium 141 vitamin solution per Archived DSMZ Medium 135; the vitamin stock contains 2.00 mg/L Biotin, yielding 0.000034884 g/L after the DSMZ 787 stock additions.
22	Folic acid C19H19N7O6 441.404 g/mol	0.000034884 G_PER_L	CHEBI:27470 Folic acid	Archived DSMZ Medium 787 uses 20 mL of Archived DSMZ Medium 141 vitamin solution per Archived DSMZ Medium 135; the vitamin stock contains 2.00 mg/L Folic acid, yielding 0.000034884 g/L after the DSMZ 787 stock additions.
23	Pyridoxine-HCl C8H11NO3.HCl 205.641 g/mol	0.000174419 G_PER_L	CHEBI:30961 Pyridoxine hydrochloride	Archived DSMZ Medium 787 uses 20 mL of Archived DSMZ Medium 141 vitamin solution per Archived DSMZ Medium 135; the vitamin stock contains 10.00 mg/L Pyridoxine-HCl, yielding 0.000174419 g/L after the DSMZ 787 stock additions.
24	Thiamine-HCl x 2 H2O C12H18N4OS.2Cl.2H2O 373.306 g/mol	0.000087209 G_PER_L	CHEBI:132751 Thiamine-HCl x 2 H2O	Archived DSMZ Medium 787 uses 20 mL of Archived DSMZ Medium 141 vitamin solution per Archived DSMZ Medium 135; the vitamin stock contains 5.00 mg/L Thiamine-HCl x 2 H2O, yielding 0.000087209 g/L after the DSMZ 787 stock additions.
25	Riboflavin C17H20N4O6 376.369 g/mol	0.000087209 G_PER_L	CHEBI:17015 Riboflavin	Archived DSMZ Medium 787 uses 20 mL of Archived DSMZ Medium 141 vitamin solution per Archived DSMZ Medium 135; the vitamin stock contains 5.00 mg/L Riboflavin, yielding 0.000087209 g/L after the DSMZ 787 stock additions.
26	Nicotinic acid C6H5NO2 123.111 g/mol	0.000087209 G_PER_L	CHEBI:15940 Nicotinic acid	Archived DSMZ Medium 787 uses 20 mL of Archived DSMZ Medium 141 vitamin solution per Archived DSMZ Medium 135; the vitamin stock contains 5.00 mg/L Nicotinic acid, yielding 0.000087209 g/L after the DSMZ 787 stock additions.
27	Calcium pantothenate 2C9H16NO5.Ca 476.536 g/mol	0.000087209 G_PER_L	CHEBI:31345 Calcium pantothenate	Archived DSMZ Medium 787 uses 20 mL of Archived DSMZ Medium 141 vitamin solution per Archived DSMZ Medium 135; the vitamin stock contains 5.00 mg/L D-Ca-pantothenate, yielding 0.000087209 g/L after the DSMZ 787 stock additions.
28	Vitamin B12	0.000001744 G_PER_L	CHEBI:176843 Vitamin B12	Archived DSMZ Medium 787 uses 20 mL of Archived DSMZ Medium 141 vitamin solution per Archived DSMZ Medium 135; the vitamin stock contains 0.10 mg/L Vitamin B12, yielding 0.000001744 g/L after the DSMZ 787 stock additions.
29	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	0.000087209 G_PER_L	CHEBI:30753 p-Aminobenzoic acid	Archived DSMZ Medium 787 uses 20 mL of Archived DSMZ Medium 141 vitamin solution per Archived DSMZ Medium 135; the vitamin stock contains 5.00 mg/L p-Aminobenzoic acid, yielding 0.000087209 g/L after the DSMZ 787 stock additions.
30	(DL)-alpha-Lipoic acid C8H14O2S2 206.332 g/mol	0.000087209 G_PER_L	CHEBI:16494 (DL)-alpha-Lipoic acid	Archived DSMZ Medium 787 uses 20 mL of Archived DSMZ Medium 141 vitamin solution per Archived DSMZ Medium 135; the vitamin stock contains 5.00 mg/L Lipoic acid, yielding 0.000087209 g/L after the DSMZ 787 stock additions.
31	NaHCO3 CHO3.Na 84.006 g/mol	9.689922 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Archived DSMZ Medium 787 adds 1.00 mL of 10% w/v NaHCO3 stock per 9 mL base medium.
32	Cysteine-HCl x H2O C3H7NO2S.H2O.HCl 175.637 g/mol	0.494186 G_PER_L	CHEBI:91248 L-cysteine hydrochloride hydrate	Archived DSMZ Medium 787 adds 0.17 mL of 3% w/v Cysteine-HCl x H2O stock per 9 mL base medium.
33	Na-syringate C9H9O5.Na 220.156 g/mol	0.872093 G_PER_L	CHEBI:132110 sodium syringate	Archived DSMZ Medium 787 adds 0.15 mL of 6% w/v Na-syringate stock per 9 mL base medium.
34	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Archived DSMZ Medium 787 prepares the base medium under 80% N2 plus 20% CO2.
35	CO2 CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Archived DSMZ Medium 787 prepares the base medium under 80% N2 plus 20% CO2.
36	Distilled water H2O 18.015 g/mol	900.000 ML_PER_L	CHEBI:15377 water	Archived DSMZ Medium 787 dissolves the modified Medium 135 ingredients in 900 mL water.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:03.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 787
2026-03-13T06:19:41.250214Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:006488
2026-03-17T19:45:58.682098+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "ACETOBACTERIUM DEHALOGENANS medium" to "acetobacterium_dehalogenans_medium" for consistent matching
2026-08-06T08:58:32.379545+00:00	curate_flag_empty_composition.py	FLAGGED_INCOMPLETE_COMPOSITION	Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175).
2026-09-10T00:00:00-07:00	repair_komodo_787_score30.py	RESOLVED_KOMODO_787_SCORE30	Archived DSMZ Medium 787 defines ACETOBACTERIUM DEHALOGENANS MEDIUM as DSMZ Medium 135 prepared without fructose, sodium sulfide, or bicarbonate in 900 mL water under 80:20 N2/CO2, with NaHCO3, Cysteine-HCl x H2O, and Na-syringate added to each 9 mL aliquot from sterile anaerobic stocks; this record expands the Medium 135 trace and vitamin stocks into final per-liter components after the 10.32 mL final aliquot volume.