for_dsm_16260 (CultureMech:006494)

Overview

External ontology

komodo.medium:78b.1 (source link unavailable) — For DSM 16260

Applications

Microbial cultivation

pH

7.0

Notes

Source: KOMODO ModelSEED | ID: 78b.1 | DSMZ Medium: 78b (mediadive.medium:78b) | Aerobic: Yes

Composition

flowchart LR
    CultureMech_006494["for_dsm_16260"]
    ing0_Ribose["Ribose"]
    CultureMech_006494 --> ing0_Ribose
    CHEBI_16988["CHEBI:16988"]
    ing0_Ribose -.-> CHEBI_16988
    ing1_Glucuronic_acid["Glucuronic acid"]
    CultureMech_006494 --> ing1_Glucuronic_acid
    CHEBI_4178["CHEBI:4178"]
    ing1_Glucuronic_acid -.-> CHEBI_4178
    ing2_Casamino_acid["Casamino acid"]
    CultureMech_006494 --> ing2_Casamino_acid
    ing3_KCl["KCl"]
    CultureMech_006494 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_L_Glutamine["L-Glutamine"]
    CultureMech_006494 --> ing4_L_Glutamine
    CHEBI_18050["CHEBI:18050"]
    ing4_L_Glutamine -.-> CHEBI_18050
    ing5_Na_pyruvate["Na-pyruvate"]
    CultureMech_006494 --> ing5_Na_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing5_Na_pyruvate -.-> CHEBI_50144
    ing6_Casitone["Casitone"]
    CultureMech_006494 --> ing6_Casitone
    ing7_K2HPO4["K2HPO4"]
    CultureMech_006494 --> ing7_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing7_K2HPO4 -.-> CHEBI_131527
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_006494 --> ing8_Yeast_extract
    ing9_N_acetylglucosamine["N-acetylglucosamine"]
    CultureMech_006494 --> ing9_N_acetylglucosamine
    CHEBI_59640["CHEBI:59640"]
    ing9_N_acetylglucosamine -.-> CHEBI_59640
    ing10_glutathione["glutathione"]
    CultureMech_006494 --> ing10_glutathione
    CHEBI_16856["CHEBI:16856"]
    ing10_glutathione -.-> CHEBI_16856
    ing11_Tryptone_Peptone__Difco_0123_17_["Tryptone Peptone (Difco 0123-17)"]
    CultureMech_006494 --> ing11_Tryptone_Peptone__Difco_0123_17_
    ing12_Fetal_or_newborn_calf_serum["Fetal or newborn calf serum"]
    CultureMech_006494 --> ing12_Fetal_or_newborn_calf_serum
    ing13_Ascorbic_acid["Ascorbic acid"]
    CultureMech_006494 --> ing13_Ascorbic_acid
    CHEBI_22652["CHEBI:22652"]
    ing13_Ascorbic_acid -.-> CHEBI_22652
    ing14_hemin["hemin"]
    CultureMech_006494 --> ing14_hemin
    CHEBI_50385["CHEBI:50385"]
    ing14_hemin -.-> CHEBI_50385
    ing15_Cysteine["Cysteine"]
    CultureMech_006494 --> ing15_Cysteine
    CHEBI_17561["CHEBI:17561"]
    ing15_Cysteine -.-> CHEBI_17561
    ing16_Resazurin["Resazurin"]
    CultureMech_006494 --> ing16_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing16_Resazurin -.-> CHEBI_8806
    ing17_L_Serine["L-Serine"]
    CultureMech_006494 --> ing17_L_Serine
    CHEBI_17115["CHEBI:17115"]
    ing17_L_Serine -.-> CHEBI_17115
    ing18_L_Histidine["L-Histidine"]
    CultureMech_006494 --> ing18_L_Histidine
    CHEBI_15971["CHEBI:15971"]
    ing18_L_Histidine -.-> CHEBI_15971
    ing19_Ground_beef__fat_free_["Ground beef (fat free)"]
    CultureMech_006494 --> ing19_Ground_beef__fat_free_

Ingredients (20)

#	Ingredient	Concentration	Ontology	Notes
1	Ribose C5H10O5 150.13 g/mol	1.91 G_PER_L	CHEBI:16988 D-ribose	KOMODO Medium 78b.1 lists 1.91 g/L Ribose in the final-component table.
2	Glucuronic acid C6H10O7 194.139 g/mol	1.91 G_PER_L	CHEBI:4178 D-glucuronic acid	KOMODO Medium 78b.1 lists 1.91 g/L Glucuronic acid in the final-component table.
3	Casamino acid	0.15 G_PER_L	FOODON:03315719 mammalian milk protein (hydrolyzed)	KOMODO Medium 78b.1 lists 0.15 g/L Casamino acid in the final-component table.
4	KCl Cl.K 74.551 g/mol	0.48 G_PER_L	CHEBI:32588 potassium chloride	KOMODO Medium 78b.1 lists 0.48 g/L KCl in the final-component table.
5	L-Glutamine C5H10N2O3 146.146 g/mol	0.67 G_PER_L	CHEBI:18050 L-glutamine	KOMODO Medium 78b.1 lists 0.67 g/L L-Glutamine in the final-component table.
6	Na-pyruvate C3H3O3.Na 110.044 g/mol	0.48 G_PER_L	CHEBI:50144 sodium pyruvate	KOMODO Medium 78b.1 lists 0.48 g/L Na-pyruvate in the final-component table.
7	Casitone	28.01 G_PER_L		KOMODO Medium 78b.1 lists 28.01 g/L Casitone in the final-component table.
8	K2HPO4 HO4P.2K 174.174 g/mol	4.67 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	KOMODO Medium 78b.1 lists 4.67 g/L K2HPO4 in the final-component table.
9	Yeast extract	4.82 G_PER_L	FOODON:03315426 yeast extract	KOMODO Medium 78b.1 lists 4.82 g/L Yeast extract in the final-component table.
10	N-acetylglucosamine	0.48 G_PER_L	CHEBI:59640 N-acetylglucosamine	KOMODO Medium 78b.1 lists 0.48 g/L N-acetylglucosamine in the final-component table.
11	glutathione C10H17N3O6S 307.328 g/mol	1.34 G_PER_L	CHEBI:16856 glutathione	KOMODO Medium 78b.1 lists 1.34 g/L glutathione in the final-component table.
12	Tryptone Peptone (Difco 0123-17)	0.15 G_PER_L	FOODON:03315719 mammalian milk protein (hydrolyzed)	KOMODO Medium 78b.1 lists 0.15 g/L Tryptone Peptone (Difco 0123-17) in the final-component table.
13	Fetal or newborn calf serum	9.57 G_PER_L		KOMODO Medium 78b.1 lists 9.57 g/L Fetal or newborn calf serum in the final-component table.
14	Ascorbic acid	0.10 G_PER_L	CHEBI:22652 ascorbic acid	KOMODO Medium 78b.1 lists 0.10 g/L Ascorbic acid in the final-component table.
15	hemin C34H32ClFeN4O4 651.952 g/mol	4.78E-4 G_PER_L	CHEBI:50385 hemin	KOMODO Medium 78b.1 lists 4.78E-4 g/L hemin in the final-component table.
16	Cysteine C3H7NO2S 121.161 g/mol	0.47 G_PER_L	CHEBI:17561 L-cysteine	KOMODO Medium 78b.1 lists 0.47 g/L Cysteine in the final-component table.
17	Resazurin C12H7NO4 229.191 g/mol	9.34E-4 G_PER_L	CHEBI:8806 Resazurin	KOMODO Medium 78b.1 lists 9.34E-4 g/L Resazurin in the final-component table.
18	L-Serine C3H7NO3 105.093 g/mol	0.48 G_PER_L	CHEBI:17115 L-serine	KOMODO Medium 78b.1 lists 0.48 g/L L-Serine in the final-component table.
19	L-Histidine C6H9N3O2 155.157 g/mol	0.57 G_PER_L	CHEBI:15971 L-histidine	KOMODO Medium 78b.1 lists 0.57 g/L L-Histidine in the final-component table.
20	Ground beef (fat free)	466.80 G_PER_L		KOMODO Medium 78b.1 lists 466.80 g/L Ground beef (fat free) in the final-component table.

Preparation

DSMZ Medium 78b boils lean chopped beef or horse meat with water and NaOH for 15 min, filters while retaining meat particles and filtrate, adds Casitone, yeast extract, K2HPO4, resazurin, and water to 700 ml, sparges with 80% N2/20% CO2 for 30-45 min, adds L-cysteine HCl x H2O, adjusts pH to 7.0, dispenses under the same gas into Hungate tubes with meat particles, and autoclaves at 121 C for 20 min.
When the DSMZ catalogue specifies Haemin, Vitamin K1, or Vitamin K3, prepare those stocks separately, filter-sterilize them, and add them to the autoclaved chopped-meat medium.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:03.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 78b.1
2026-02-03T11:19:07.530396	dsmz-resolver-v1.0	Enriched with DSMZ composition	Copied ingredients from DSMZ Medium 78
2026-03-13T03:09:12.201117Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 2 duplicate ingredient(s)
2026-03-13T06:19:41.307027Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:006494
2026-03-17T06:30:19.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:58.747006+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "For DSM 16260" to "for_dsm_16260" for consistent matching
2026-06-05T07:41:02.388317+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:57.615923+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-12T00:00:00-07:00	repair_dsmz_78b_treponema_score15.py	RESOLVED_DSMZ_KOMODO_78B_SCORE15	KOMODO Medium 78b.1 records the DSM 16260 strain variant of the Treponema parvum formulation.