Overview
- External ontology
- komodo.medium:792.2 (source link unavailable) — DSM 16658
- Applications
-
- Microbial cultivation
- pH
- 5.7
- Notes
- Source: KOMODO ModelSEED | ID: 792.2 | DSMZ Medium: 792 (mediadive.medium:792) | Aerobic: Yes
Composition
flowchart LR
CultureMech_006500["dsm_16658"]
ing0_NaCl["NaCl"]
CultureMech_006500 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006500 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_006500 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_006500 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_KCl["KCl"]
CultureMech_006500 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_NaBr["NaBr"]
CultureMech_006500 --> ing5_NaBr
CHEBI_63004["CHEBI:63004"]
ing5_NaBr -.-> CHEBI_63004
ing6_H3BO3["H3BO3"]
CultureMech_006500 --> ing6_H3BO3
CHEBI_33118["CHEBI:33118"]
ing6_H3BO3 -.-> CHEBI_33118
ing7_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_006500 --> ing7_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing7_SrCl2_x_6_H2O -.-> CHEBI_36385
ing8_KI["KI"]
CultureMech_006500 --> ing8_KI
CHEBI_8346["CHEBI:8346"]
ing8_KI -.-> CHEBI_8346
ing9_Sodium_resazurin["Sodium resazurin"]
CultureMech_006500 --> ing9_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Sodium_resazurin -.-> CHEBI_8806
ing10_KNO3["KNO3"]
CultureMech_006500 --> ing10_KNO3
CHEBI_63043["CHEBI:63043"]
ing10_KNO3 -.-> CHEBI_63043
ing11_KH2PO4["KH2PO4"]
CultureMech_006500 --> ing11_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing11_KH2PO4 -.-> CHEBI_63036
ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_006500 --> ing12_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing12_Na2S_x_9_H2O -.-> CHEBI_76209
ing13_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_006500 --> ing13_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing13_CuSO4_x_5_H2O -.-> CHEBI_31440
ing14_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_006500 --> ing14_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing14_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_006500 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing16__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
CultureMech_006500 --> ing16__NH4_2Ni_SO4_2_x_6_H2O
CHEBI_86149["CHEBI:86149"]
ing16__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
ing17_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_006500 --> ing17_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing17_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing18_Na2SeO4["Na2SeO4"]
CultureMech_006500 --> ing18_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing18_Na2SeO4 -.-> CHEBI_77775
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
40.3337 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 30.3337, 10.0] |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
33.53893 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 3.53893, 30.0] |
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.78059 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.7583419999999998 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.758342, 1.0] |
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.33367 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
NaBr
Br.Na 102.894 g/mol
|
0.0505561 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 7 |
H3BO3
H3BO3 61.833 g/mol
|
0.1151668 G_PER_L |
CHEBI:33118
boric acid
|
[Merged 2 duplicates: 0.0151668, 0.1] |
| 8 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.00707786 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 9 |
KI
I.K 166.002 g/mol
|
5.05561e-05 G_PER_L |
CHEBI:8346
potassium iodide
|
|
| 10 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.000505561 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 11 |
KNO3
K.NO3 101.102 g/mol
|
100 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 12 |
KH2PO4
H2O4P.K 136.084 g/mol
|
50 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 13 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
15 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 14 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.1 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 15 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.18 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 17 |
(NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
2.8 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
|
| 18 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.1 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 19 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.1 G_PER_L |
CHEBI:77775
sodium selenate
|
Solutions
Wolfe's mineral elixir
| Ingredient | Concentration | Notes |
|---|---|---|
| MnSO4 x H2O | 5 G_PER_L | |
| FeSO4 x 7 H2O | 1 G_PER_L | |
| CoCl2 x 6 H2O | 1.8 G_PER_L | |
| ZnSO4 x 7 H2O | 1.8 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 792.2 |
| 2026-02-03T11:19:15.423407 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 792 |
| 2026-03-13T03:09:33.013945Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:41.366466Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006500 |
| 2026-03-17T06:30:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:58.814688+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DSM 16658" to "dsm_16658" for consistent matching |
| 2026-06-05T07:40:59.065102+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.749606+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:56.534588+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-15T00:17:29.037531+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 4 stock-strength component(s) out of ingredients into 1 solution(s): 4 -> "Wolfe's mineral elixir" @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |
| 2026-09-13T00:00:00-07:00 | repair_komodo_792_pyrolobus_score10.py | RESOLVED_KOMODO_792_PYROLOBUS_TOPOLOGY | KOMODO Medium 792.2 applies Pyrolobus fumarii medium to DSM 16658. |