Overview
- External ontology
- komodo.medium:803 (source link unavailable) — SPHAEROTILUS-LEPTOTHRIX medium
- Applications
-
- Microbial cultivation
- pH
- 7.1
- Notes
- Source: KOMODO ModelSEED | ID: 803 | DSMZ Medium: 803 (mediadive.medium:803) | Aerobic: No
Composition
flowchart LR
CultureMech_006520["sphaerotilus_leptothrix_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_006520 --> ing0_Yeast_extract
ing1_Peptone["Peptone"]
CultureMech_006520 --> ing1_Peptone
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006520 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2["CaCl2"]
CultureMech_006520 --> ing3_CaCl2
CHEBI_3312["CHEBI:3312"]
ing3_CaCl2 -.-> CHEBI_3312
ing4_Ferric_ammonium_citrate["Ferric ammonium citrate"]
CultureMech_006520 --> ing4_Ferric_ammonium_citrate
CHEBI_31604["CHEBI:31604"]
ing4_Ferric_ammonium_citrate -.-> CHEBI_31604
ing5_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_006520 --> ing5_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing5_MnSO4_x_H2O -.-> CHEBI_86364
ing6_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_006520 --> ing6_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing6_FeCl3_x_6_H2O -.-> CHEBI_86254
ing7_Agar["Agar"]
CultureMech_006520 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
ing8_Tap_water["Tap water"]
CultureMech_006520 --> ing8_Tap_water
CHEBI_15377["CHEBI:15377"]
ing8_Tap_water -.-> CHEBI_15377
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 1 G_PER_L | ||
| 2 | Peptone | 1.5 G_PER_L | ||
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.05 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 5 | Ferric ammonium citrate | 0.5 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
|
| 6 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.05 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 7 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.01 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 8 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
|
| 9 |
Tap water
H2O 18.015 g/mol
|
1 L |
CHEBI:15377
water
|
Source batch input: 1000 ml tap water = 1 L. This is an input volume, not a mass concentration, volume percentage or measured final-medium volume. No density or final-volume assumption is made. |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 803 |
| 2026-02-03T11:19:03.017861 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 803 |
| 2026-03-13T06:19:41.512985Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006520 |
| 2026-03-17T06:30:04.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:58.984353+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SPHAEROTILUS-LEPTOTHRIX medium" to "sphaerotilus_leptothrix_medium" for consistent matching |
| 2026-06-05T07:40:26.578655+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-10-08T06:29:49.318246+00:00 | codex | CURATED_KOMODO_CONCENTRATION_SOURCE_EVIDENCE | Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. KOMODO accession 803 is mapped to the same DSMZ accession by https://komodo.modelseed.org/servlet/KomodoTomcatServerSideUtilitiesModelSeed?MediaList; mapping response SHA256=d8259d98e6dbffdf6961a1ab92bc86f3567d5d96de352bfa079edb2a91e9580a. The mapping supports identity only, not ingredient quantities or variants. |
| 2026-10-08T07:20:58.579071+00:00 | codex | CORRECTED_DSMZ803_1046_1065_WATER_INPUT_UNITS | Original DSMZ PDF and exact linked API recipe independently inspected. Preserved prior evidence/history, identifiers, non-water amounts, preparation, chemical mappings and parent/variant links. No final-volume, density, stock dilution or growth inference. |