Overview
- External ontology
- komodo.medium:815 (source link unavailable) — DESULFOTOMACULUM HALOPHILUM medium
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- pH buffer: NaOH | Source: KOMODO ModelSEED | ID: 815 | DSMZ Medium: 815 (mediadive.medium:815) | Aerobic: No
Composition
flowchart LR
CultureMech_006539["desulfotomaculum_halophilum_medium"]
ing0_Na2SO4["Na2SO4"]
CultureMech_006539 --> ing0_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing0_Na2SO4 -.-> CHEBI_32149
ing1_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_006539 --> ing1_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_x_2_H2O -.-> CHEBI_86158
ing2_NaCl["NaCl"]
CultureMech_006539 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_KCl["KCl"]
CultureMech_006539 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_NH4Cl["NH4Cl"]
CultureMech_006539 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_KH2PO4["KH2PO4"]
CultureMech_006539 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_006539 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
ing7_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_006539 --> ing7_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing7_SrCl2_x_6_H2O -.-> CHEBI_36385
ing8_MOPS["MOPS"]
CultureMech_006539 --> ing8_MOPS
CHEBI_39074["CHEBI:39074"]
ing8_MOPS -.-> CHEBI_39074
ing9_Yeast_extract["Yeast extract"]
CultureMech_006539 --> ing9_Yeast_extract
ing10_Sodium_resazurin["Sodium resazurin"]
CultureMech_006539 --> ing10_Sodium_resazurin
CHEBI_8806["CHEBI:8806"]
ing10_Sodium_resazurin -.-> CHEBI_8806
ing11_Na_pyruvate["Na-pyruvate"]
CultureMech_006539 --> ing11_Na_pyruvate
CHEBI_50144["CHEBI:50144"]
ing11_Na_pyruvate -.-> CHEBI_50144
ing12_Na2CO3["Na2CO3"]
CultureMech_006539 --> ing12_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing12_Na2CO3 -.-> CHEBI_29377
ing13_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_006539 --> ing13_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing13_Na2S_x_9_H2O -.-> CHEBI_76209
ing14_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
CultureMech_006539 --> ing14_Na2_EDTA_x_2_H2O
CHEBI_64758["CHEBI:64758"]
ing14_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
ing15_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_006539 --> ing15_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing15_FeCl2_x_4_H2O -.-> CHEBI_86249
ing16_ZnCl2["ZnCl2"]
CultureMech_006539 --> ing16_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing16_ZnCl2 -.-> CHEBI_49976
ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_006539 --> ing17_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
ing18_H3BO3["H3BO3"]
CultureMech_006539 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_006539 --> ing19_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_006539 --> ing20_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_006539 --> ing21_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_006539 --> ing22_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing23_NaOH["NaOH"]
CultureMech_006539 --> ing23_NaOH
CHEBI_32145["CHEBI:32145"]
ing23_NaOH -.-> CHEBI_32145
Ingredients (24)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 2 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
6 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
40 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.3 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
8 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 8 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.1 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 9 | MOPS | 3 G_PER_L |
CHEBI:39074
MOPS
|
|
| 10 | Yeast extract | 1 G_PER_L | ||
| 11 |
Sodium resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 12 |
Na-pyruvate
C3H3O3.Na 110.044 g/mol
|
2.5 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 13 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.5 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 14 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.1 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 15 |
Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
|
5.2 G_PER_L |
CHEBI:64758
EDTA disodium salt dihydrate
|
|
| 16 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 17 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 18 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 20 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 21 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 22 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 23 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 24 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 815 |
| 2026-02-03T11:19:12.550705 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 815 |
| 2026-03-13T03:09:25.960319Z | data-quality-cleanup-v1.0 | Data quality fixes | Added pH buffer: NaOH |
| 2026-03-13T06:19:41.673993Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006539 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-17T06:30:35.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:59.170526+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFOTOMACULUM HALOPHILUM medium" to "desulfotomaculum_halophilum_medium" for consistent matching |
| 2026-05-16T09:03:30.032518+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:57.562718+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:55.942561+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-13T00:00:00-07:00 | repair_komodo_815_desulfotomaculum_score10.py | RESOLVED_KOMODO_815_DESULFOTOMACULUM_TOPOLOGY | Linked the strain-specific KOMODO Medium 815.1 record under the KOMODO Medium 815 DESULFOTOMACULUM HALOPHILUM base. |