for_dsm_16346

CultureMech:006542 bacterial DEFINED LIQUID
Original name: For DSM 16346

Overview

External ontology
komodo.medium:818.1 (source link unavailable) — For DSM 16346
Applications
  • Microbial cultivation
pH range
{'min': 7.4, 'max': 7.8}
Notes
Source: KOMODO ModelSEED | ID: 818.1 | DSMZ Medium: 818 (mediadive.medium:818) | Aerobic: Yes

Composition

flowchart LR
    CultureMech_006542["for_dsm_16346"]
    ing0_NaCl["NaCl"]
    CultureMech_006542 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KCl["KCl"]
    CultureMech_006542 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_NH4Cl["NH4Cl"]
    CultureMech_006542 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_KH2PO4["KH2PO4"]
    CultureMech_006542 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_Na2SO4["Na2SO4"]
    CultureMech_006542 --> ing4_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing4_Na2SO4 -.-> CHEBI_32149
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_006542 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_006542 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_Sodium_resazurin["Sodium resazurin"]
    CultureMech_006542 --> ing7_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Sodium_resazurin -.-> CHEBI_8806
    ing8_NaHCO3["NaHCO3"]
    CultureMech_006542 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_KNO3["KNO3"]
    CultureMech_006542 --> ing9_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing9_KNO3 -.-> CHEBI_63043
    ing10_Na_acetate["Na-acetate"]
    CultureMech_006542 --> ing10_Na_acetate
    CHEBI_32954["CHEBI:32954"]
    ing10_Na_acetate -.-> CHEBI_32954
    ing11_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
    CultureMech_006542 --> ing11_Na2_EDTA_x_2_H2O
    CHEBI_64758["CHEBI:64758"]
    ing11_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
    ing12_ZnCl2["ZnCl2"]
    CultureMech_006542 --> ing12_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing12_ZnCl2 -.-> CHEBI_49976
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_006542 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_H3BO3["H3BO3"]
    CultureMech_006542 --> ing14_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing14_H3BO3 -.-> CHEBI_33118
    ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_006542 --> ing15_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_006542 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_006542 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_006542 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_Biotin["Biotin"]
    CultureMech_006542 --> ing19_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing19_Biotin -.-> CHEBI_15956
    ing20_Folic_acid["Folic acid"]
    CultureMech_006542 --> ing20_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing20_Folic_acid -.-> CHEBI_27470
    ing21_Thiamine_HCl["Thiamine HCl"]
    CultureMech_006542 --> ing21_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing21_Thiamine_HCl -.-> CHEBI_49105
    ing22_Riboflavin["Riboflavin"]
    CultureMech_006542 --> ing22_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing22_Riboflavin -.-> CHEBI_17015
    ing23_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
    CultureMech_006542 --> ing23_Calcium_D_____pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing23_Calcium_D_____pantothenate -.-> CHEBI_31345
    ing24__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
    CultureMech_006542 --> ing24__DL__alpha_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing24__DL__alpha_Lipoic_acid -.-> CHEBI_16494
    ing25_D_____biotin["D-(+)-biotin"]
    CultureMech_006542 --> ing25_D_____biotin
    ing25_D_____biotin -.-> CHEBI_15956
    ing26_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_006542 --> ing26_Calcium_pantothenate
    ing26_Calcium_pantothenate -.-> CHEBI_31345

Ingredients (27)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
1.19641 G_PER_L CHEBI:26710
sodium chloride
2 KCl
Cl.K 74.551 g/mol
0.299103 G_PER_L CHEBI:32588
potassium chloride
3 NH4Cl
Cl.H4N 53.492 g/mol
0.299103 G_PER_L CHEBI:31206
ammonium chloride
4 KH2PO4
H2O4P.K 136.084 g/mol
0.199402 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 Na2SO4
2Na.O4S 142.043 g/mol
0.299103 G_PER_L CHEBI:32149
sodium sulfate
6 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.398804 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.149551 G_PER_L CHEBI:86158
calcium chloride dihydrate
8 Sodium resazurin
C12H7NO4 229.191 g/mol
0.000498504 G_PER_L CHEBI:8806
Resazurin
9 NaHCO3
CHO3.Na 84.006 g/mol
0.598205 G_PER_L CHEBI:32139
sodium hydrogencarbonate
10 KNO3
K.NO3 101.102 g/mol
0.498504 G_PER_L CHEBI:63043
potassium nitrate
11 Na-acetate
C2H3O2.Na 82.034 g/mol
0.498504 G_PER_L CHEBI:32954
sodium acetate
12 Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
5.2 G_PER_L CHEBI:64758
EDTA disodium salt dihydrate
13 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
16 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
17 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
18 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
20 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
21 Folic acid
C19H19N7O6 441.404 g/mol
0.02 G_PER_L CHEBI:27470
folic acid
22 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.05 G_PER_L CHEBI:49105
thiamine hydrochloride
23 Riboflavin
C17H20N4O6 376.369 g/mol
0.05 G_PER_L CHEBI:17015
riboflavin
24 Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.05 G_PER_L CHEBI:31345
Calcium pantothenate
25 (DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
0.05 G_PER_L CHEBI:16494
lipoic acid
26 D-(+)-biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
27 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.1 G_PER_L CHEBI:31345
Calcium pantothenate

Solutions

Trace element solution SL-11

Stock composition
IngredientConcentrationNotes
FeCl2 x 4 H2O 1.5 G_PER_L

Seven vitamins solution

Stock composition
IngredientConcentrationNotes
Thiamine-HCl x 2 H2O 0.2 G_PER_L
Nicotinic acid 0.2 G_PER_L [Merged 2 duplicates: 0.05, 0.2]
p-Aminobenzoic acid 0.08 G_PER_L [Merged 2 duplicates: 0.05, 0.08]
Pyridoxine hydrochloride 0.3 G_PER_L [Merged 2 duplicates: 0.1, 0.3]
Vitamin B12 0.1 G_PER_L [Merged 2 duplicates: 0.001, 0.1]

Wolin's vitamin solution (10x)

Stock composition
IngredientConcentrationNotes
Nicotinic acid 0.05 G_PER_L [Merged 2 duplicates: 0.05, 0.2]
p-Aminobenzoic acid 0.05 G_PER_L [Merged 2 duplicates: 0.05, 0.08]
Pyridoxine hydrochloride 0.1 G_PER_L [Merged 2 duplicates: 0.1, 0.3]
Vitamin B12 0.001 G_PER_L [Merged 2 duplicates: 0.001, 0.1]

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:03.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 818.1
2026-02-03T11:19:08.982057 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 818
2026-02-06T05:10:36.526123Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T03:09:15.758843Z data-quality-cleanup-v1.0 Data quality fixes Merged 4 duplicate ingredient(s)
2026-03-13T06:19:41.707477Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:006542
2026-03-17T06:30:23.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:59.208143+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "For DSM 16346" to "for_dsm_16346" for consistent matching
2026-05-20T06:01:18.261971+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.4-7.8' -> {'min': 7.4, 'max': 7.8}
2026-06-05T07:41:02.405432+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.832416+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:57.621257+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-15T00:17:30.211099+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 6 stock-strength component(s) out of ingredients into 3 solution(s): 1 -> 'Trace element solution SL-11' @ 1 ml/l; 1 -> 'Seven vitamins solution' @ 1 ml/l; 0 -> "Wolin's vitamin solution (10x)" @ 1 ml/l. 4 component(s) were SUMMED across stocks by the flattening and are restored to each stock's own value, verified by exact sum: nicotinic acid, p-aminobenzoic acid, pyridoxine hydrochloride, vitamin b12. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).
2026-09-13T00:00:00-07:00 repair_komodo_818_chrysiogenes_score10.py RESOLVED_KOMODO_818_CHRYSIOGENES_STRAIN_VARIANT KOMODO Medium 818.1 preserves CHRYSIOGENES MEDIUM components and pH 7.4-7.8 but applies the recipe for DSM 16346.