aeropyrum_jxt_medium (CultureMech:006547)

Overview

External ontology

komodo.medium:820 (source link unavailable) — AEROPYRUM-JXT medium

Applications

Microbial cultivation

pH range

{'min': 7.0, 'max': 7.2}

Notes

Archived DSMZ Medium 820 defines AEROPYRUM-JXT MEDIUM as DSMZ Medium 514, with final pH adjusted to 7.0-7.2 and 1.0 g/L sodium thiosulfate pentahydrate added from a filter-sterilized stock after autoclaving; DSMZ Medium 514 lists the Bacto Marine Broth 2216 component recipe.

Composition

flowchart LR
    CultureMech_006547["aeropyrum_jxt_medium"]
    ing0_Bacto_peptone["Bacto peptone"]
    CultureMech_006547 --> ing0_Bacto_peptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_006547 --> ing1_Yeast_extract
    ing2_Fe_III__citrate["Fe(III) citrate"]
    CultureMech_006547 --> ing2_Fe_III__citrate
    CHEBI_144421["CHEBI:144421"]
    ing2_Fe_III__citrate -.-> CHEBI_144421
    ing3_NaCl["NaCl"]
    CultureMech_006547 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_MgCl2["MgCl2"]
    CultureMech_006547 --> ing4_MgCl2
    CHEBI_6636["CHEBI:6636"]
    ing4_MgCl2 -.-> CHEBI_6636
    ing5_Na2SO4["Na2SO4"]
    CultureMech_006547 --> ing5_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing5_Na2SO4 -.-> CHEBI_32149
    ing6_CaCl2["CaCl2"]
    CultureMech_006547 --> ing6_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing6_CaCl2 -.-> CHEBI_3312
    ing7_KCl["KCl"]
    CultureMech_006547 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_NaHCO3["NaHCO3"]
    CultureMech_006547 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_KBr["KBr"]
    CultureMech_006547 --> ing9_KBr
    CHEBI_32030["CHEBI:32030"]
    ing9_KBr -.-> CHEBI_32030
    ing10_SrCl2["SrCl2"]
    CultureMech_006547 --> ing10_SrCl2
    CHEBI_36383["CHEBI:36383"]
    ing10_SrCl2 -.-> CHEBI_36383
    ing11_H3BO3["H3BO3"]
    CultureMech_006547 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_Na_silicate["Na-silicate"]
    CultureMech_006547 --> ing12_Na_silicate
    CHEBI_60720["CHEBI:60720"]
    ing12_Na_silicate -.-> CHEBI_60720
    ing13_NaF["NaF"]
    CultureMech_006547 --> ing13_NaF
    CHEBI_28741["CHEBI:28741"]
    ing13_NaF -.-> CHEBI_28741
    ing14__NH4_NO3["(NH4)NO3"]
    CultureMech_006547 --> ing14__NH4_NO3
    CHEBI_63038["CHEBI:63038"]
    ing14__NH4_NO3 -.-> CHEBI_63038
    ing15_Na2HPO4["Na2HPO4"]
    CultureMech_006547 --> ing15_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing15_Na2HPO4 -.-> CHEBI_34683
    ing16_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_006547 --> ing16_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing16_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing17_Distilled_water["Distilled water"]
    CultureMech_006547 --> ing17_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing17_Distilled_water -.-> CHEBI_15377

Ingredients (18)

#	Ingredient	Concentration	Ontology	Notes
1	Bacto peptone	5.000000 G_PER_L	MICRO:0000178 (source link unavailable) Bacto peptone	Archived DSMZ Medium 514 lists 5.00 g Bacto peptone in 1000 mL distilled water.
2	Yeast extract	1.000000 G_PER_L	FOODON:03315426 yeast extract	Archived DSMZ Medium 514 lists 1.00 g Bacto yeast extract in 1000 mL distilled water.
3	Fe(III) citrate C6H5FeO7 244.944 g/mol	0.100000 G_PER_L	CHEBI:144421 iron(III) citrate	Archived DSMZ Medium 514 lists 0.10 g Fe(III) citrate in 1000 mL distilled water.
4	NaCl Cl.Na 58.443 g/mol	19.450000 G_PER_L	CHEBI:26710 sodium chloride	Archived DSMZ Medium 514 lists 19.45 g NaCl in 1000 mL distilled water.
5	MgCl2 2Cl.Mg 95.211 g/mol	5.900000 G_PER_L	CHEBI:6636 magnesium dichloride	Archived DSMZ Medium 514 lists 5.90 g MgCl2 (anhydrous) in 1000 mL distilled water.
6	Na2SO4 2Na.O4S 142.043 g/mol	3.240000 G_PER_L	CHEBI:32149 sodium sulfate	Archived DSMZ Medium 514 lists 3.24 g Na2SO4 in 1000 mL distilled water.
7	CaCl2 Ca.2Cl 110.984 g/mol	1.800000 G_PER_L	CHEBI:3312 calcium dichloride	Archived DSMZ Medium 514 lists 1.80 g CaCl2 in 1000 mL distilled water.
8	KCl Cl.K 74.551 g/mol	0.550000 G_PER_L	CHEBI:32588 potassium chloride	Archived DSMZ Medium 514 lists 0.55 g KCl in 1000 mL distilled water.
9	NaHCO3 CHO3.Na 84.006 g/mol	0.160000 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Archived DSMZ Medium 514 lists 0.16 g NaHCO3 in 1000 mL distilled water.
10	KBr Br.K 119.002 g/mol	0.080000 G_PER_L	CHEBI:32030 potassium bromide	Archived DSMZ Medium 514 lists 0.08 g KBr in 1000 mL distilled water.
11	SrCl2 2Cl.Sr 158.526 g/mol	0.034000 G_PER_L	CHEBI:36383 strontium dichloride	Archived DSMZ Medium 514 lists 34.00 mg SrCl2 in 1000 mL distilled water, converted to 0.034000 g/L.
12	H3BO3 H3BO3 61.833 g/mol	0.022000 G_PER_L	CHEBI:33118 boric acid	Archived DSMZ Medium 514 lists 22.00 mg H3BO3 in 1000 mL distilled water, converted to 0.022000 g/L.
13	Na-silicate 2Na.O3Si 122.063 g/mol	0.004000 G_PER_L	CHEBI:60720 sodium silicate	Archived DSMZ Medium 514 lists 4.00 mg Na-silicate in 1000 mL distilled water, converted to 0.004000 g/L.
14	NaF F.Na 41.988 g/mol	0.002400 G_PER_L	CHEBI:28741 sodium fluoride	Archived DSMZ Medium 514 lists 2.40 mg NaF in 1000 mL distilled water, converted to 0.002400 g/L.
15	(NH4)NO3 H4N.NO3 80.043 g/mol	0.001600 G_PER_L	CHEBI:63038 ammonium nitrate	Archived DSMZ Medium 514 lists 1.60 mg (NH4)NO3 in 1000 mL distilled water, converted to 0.001600 g/L.
16	Na2HPO4 HO4P.2Na 141.958 g/mol	0.008000 G_PER_L	CHEBI:34683 disodium hydrogenphosphate	Archived DSMZ Medium 514 lists 8.00 mg Na2HPO4 in 1000 mL distilled water, converted to 0.008000 g/L.
17	Na2S2O3 x 5 H2O 5H2O.2Na.O3S2 248.186 g/mol	1.000000 G_PER_L	CHEBI:32150 sodium thiosulfate pentahydrate	Archived DSMZ Medium 820 adds 1.0 g/L Na2S2O3 x 5 H2O from a filter-sterilized stock after autoclaving DSMZ Medium 514.
18	Distilled water H2O 18.015 g/mol	1000.000 ML_PER_L	CHEBI:15377 water	Archived DSMZ Medium 514 lists 1000 mL distilled water.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:03.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 820
2026-03-13T06:19:41.751821Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:006547
2026-03-17T19:45:59.258335+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "AEROPYRUM-JXT medium" to "aeropyrum_jxt_medium" for consistent matching
2026-05-20T06:01:07.622487+00:00	migrate_ph_range.py	MIGRATED_PH_RANGE	ph_range '7.0-7.2' -> {'min': 7.0, 'max': 7.2}
2026-08-06T08:58:32.362814+00:00	curate_flag_empty_composition.py	FLAGGED_INCOMPLETE_COMPOSITION	Media record has no ingredients and no solutions — no usable composition; flagged so the gap is not silent (#175).
2026-09-10T00:00:00-07:00	repair_komodo_820_score30.py	RESOLVED_KOMODO_820_SCORE30	Archived DSMZ Medium 820 defines AEROPYRUM-JXT MEDIUM as DSMZ Medium 514, with final pH adjusted to 7.0-7.2 and 1.0 g/L sodium thiosulfate pentahydrate added from a filter-sterilized stock after autoclaving; DSMZ Medium 514 lists the Bacto Marine Broth 2216 component recipe.