Overview
- External ontology
- komodo.medium:828 (source link unavailable) — NEVSKIA MEDIUM
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.0, 'max': 7.2}
- Notes
- Source: KOMODO ModelSEED | ID: 828 | DSMZ Medium: 828 (mediadive.medium:828) | Aerobic: No
Composition
flowchart LR
CultureMech_006559["nevskia_medium"]
ing0_Potassium_phosphate_buffer["Potassium phosphate buffer"]
CultureMech_006559 --> ing0_Potassium_phosphate_buffer
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006559 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_006559 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_Na_lactate["Na-lactate"]
CultureMech_006559 --> ing3_Na_lactate
CHEBI_75228["CHEBI:75228"]
ing3_Na_lactate -.-> CHEBI_75228
ing4_Agar["Agar"]
CultureMech_006559 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
ing5_HCl["HCl"]
CultureMech_006559 --> ing5_HCl
CHEBI_17883["CHEBI:17883"]
ing5_HCl -.-> CHEBI_17883
ing6_ZnCl2["ZnCl2"]
CultureMech_006559 --> ing6_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing6_ZnCl2 -.-> CHEBI_49976
ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_006559 --> ing7_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
ing8_H3BO3["H3BO3"]
CultureMech_006559 --> ing8_H3BO3
CHEBI_33118["CHEBI:33118"]
ing8_H3BO3 -.-> CHEBI_33118
ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_006559 --> ing9_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
ing10_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_006559 --> ing10_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing10_CuCl2_x_2_H2O -.-> CHEBI_86318
ing11_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_006559 --> ing11_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing11_NiCl2_x_6_H2O -.-> CHEBI_34887
ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_006559 --> ing12_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_006559 --> ing13_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
ing14_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_006559 --> ing14_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing14_p_Aminobenzoic_acid -.-> CHEBI_30753
ing15_D_____biotin["D-(+)-biotin"]
CultureMech_006559 --> ing15_D_____biotin
CHEBI_15956["CHEBI:15956"]
ing15_D_____biotin -.-> CHEBI_15956
ing16_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_006559 --> ing16_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing16_Calcium_pantothenate -.-> CHEBI_31345
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Potassium phosphate buffer | 64.35 G_PER_L | (1M, pH 7) | |
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.05 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.014 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
Na-lactate
C3H5O3.Na 112.06 g/mol
|
0.56 G_PER_L |
CHEBI:75228
sodium lactate
|
|
| 5 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
(if necessary) |
| 6 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 7 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 8 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 9 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 10 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 11 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 12 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 13 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 14 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
12.8 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 15 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.08 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 16 |
D-(+)-biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 17 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Solutions
SL-9
Dose: 0.5 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| FeCl2 x 4 H2O | 2 G_PER_L |
Seven vitamins solution
Dose: 0.5 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Vitamin B12 | 0.1 G_PER_L | |
| Nicotinic acid | 0.2 G_PER_L | |
| Pyridoxine hydrochloride | 0.3 G_PER_L | |
| Thiamine-HCl x 2 H2O | 0.2 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 828 |
| 2026-02-03T11:19:10.430562 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 828 |
| 2026-03-13T06:19:41.890786Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006559 |
| 2026-03-17T06:30:27.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:59.412270+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NEVSKIA MEDIUM" to "nevskia_medium" for consistent matching |
| 2026-05-20T06:00:54.118184+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.0-7.2' -> {'min': 7.0, 'max': 7.2} |
| 2026-06-05T07:40:26.806683+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:46.017012+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-07T20:44:33.568125+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 5 stock-strength component(s) out of ingredients into 2 solution(s): 1 -> 'SL-9' @ 0.5 ml/l; 4 -> 'Seven vitamins solution' @ 0.5 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |