Overview
- External ontology
- komodo.medium:829a (source link unavailable) — DESULFUROBACTERIUM ATLANTICUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: KOMODO ModelSEED | ID: 829a | DSMZ Medium: 829a (mediadive.medium:829a) | Aerobic: Yes Superseded historical provenance, not a current formulation claim: Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification. Preserve the provider's aerobic metadata as a source assertion, not a biological growth claim. Its linked original specifies anoxic preparation; any disagreement remains unresolved rather than silently overriding either source. The original's anoxic instructions and stock preparations are separate source context; they do not override the provider's exact reported table rates and nulls. Full exact-source recipe and stock/preparation tables were inspected. Reconstructed nodes retain explicit source scope; complete prior ingredient/stock assertions, including copied LB mappings and stage conflations, are archived in append-only history. A title alone is not the basis for removal. Unchanged annotations are preserved, not recertified; no new organism-growth association is added.
Composition
flowchart LR
CultureMech_006565["desulfurobacterium_atlanticum_medium"]
ing0_H2O["H2O"]
CultureMech_006565 --> ing0_H2O
ing1_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_006565 --> ing1_Na2S_x_9_H2O
ing2_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_006565 --> ing2_Na2S2O3_x_5_H2O
ing3_CO2["CO2"]
CultureMech_006565 --> ing3_CO2
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_006565 --> ing4_MgCl2_x_6_H2O
ing5_KH2PO4["KH2PO4"]
CultureMech_006565 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_H2["H2"]
CultureMech_006565 --> ing6_H2
ing7_NH4Cl["NH4Cl"]
CultureMech_006565 --> ing7_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing7_NH4Cl -.-> CHEBI_31206
ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_006565 --> ing8_CaCl2_x_2_H2O
ing9_KCl["KCl"]
CultureMech_006565 --> ing9_KCl
ing10_Resazurin["Resazurin"]
CultureMech_006565 --> ing10_Resazurin
ing11_NaCl["NaCl"]
CultureMech_006565 --> ing11_NaCl
ing12_MES["MES"]
CultureMech_006565 --> ing12_MES
CHEBI_39005["CHEBI:39005"]
ing12_MES -.-> CHEBI_39005
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | H2O | Exact-accession provider medium Gram Amount is null; no fixed amount is asserted. This is a source-reported rate or null, not an inferred stock dose or a normalization from the linked DSMZ recipe. Mol Amount and SEED aggregates are not adopted. | ||
| 2 | Na2S x 9 H2O | 0.50 G_PER_L | Exact-accession provider medium Gram Amount: 0.50 g/L, preserving displayed precision. This is a source-reported rate or null, not an inferred stock dose or a normalization from the linked DSMZ recipe. Mol Amount and SEED aggregates are not adopted. | |
| 3 | Na2S2O3 x 5 H2O | 2.50 G_PER_L | Exact-accession provider medium Gram Amount: 2.50 g/L, preserving displayed precision. This is a source-reported rate or null, not an inferred stock dose or a normalization from the linked DSMZ recipe. Mol Amount and SEED aggregates are not adopted. | |
| 4 | CO2 | Exact-accession provider medium Gram Amount is null; no fixed amount is asserted. This is a source-reported rate or null, not an inferred stock dose or a normalization from the linked DSMZ recipe. Mol Amount and SEED aggregates are not adopted. | ||
| 5 | MgCl2 x 6 H2O | 3.00 G_PER_L | Exact-accession provider medium Gram Amount: 3.00 g/L, preserving displayed precision. This is a source-reported rate or null, not an inferred stock dose or a normalization from the linked DSMZ recipe. Mol Amount and SEED aggregates are not adopted. | |
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.20 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Exact-accession provider medium Gram Amount: 0.20 g/L, preserving displayed precision. This is a source-reported rate or null, not an inferred stock dose or a normalization from the linked DSMZ recipe. Mol Amount and SEED aggregates are not adopted. |
| 7 | H2 | Exact-accession provider medium Gram Amount is null; no fixed amount is asserted. This is a source-reported rate or null, not an inferred stock dose or a normalization from the linked DSMZ recipe. Mol Amount and SEED aggregates are not adopted. | ||
| 8 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.50 G_PER_L |
CHEBI:31206
ammonium chloride
|
Exact-accession provider medium Gram Amount: 0.50 g/L, preserving displayed precision. This is a source-reported rate or null, not an inferred stock dose or a normalization from the linked DSMZ recipe. Mol Amount and SEED aggregates are not adopted. |
| 9 | CaCl2 x 2 H2O | 0.15 G_PER_L | Exact-accession provider medium Gram Amount: 0.15 g/L, preserving displayed precision. This is a source-reported rate or null, not an inferred stock dose or a normalization from the linked DSMZ recipe. Mol Amount and SEED aggregates are not adopted. | |
| 10 | KCl | 0.50 G_PER_L | Exact-accession provider medium Gram Amount: 0.50 g/L, preserving displayed precision. This is a source-reported rate or null, not an inferred stock dose or a normalization from the linked DSMZ recipe. Mol Amount and SEED aggregates are not adopted. | |
| 11 | Resazurin | 5.00E-4 G_PER_L | Exact-accession provider medium Gram Amount: 5.00E-4 g/L, preserving displayed precision. This is a source-reported rate or null, not an inferred stock dose or a normalization from the linked DSMZ recipe. Mol Amount and SEED aggregates are not adopted. | |
| 12 | NaCl | 30.00 G_PER_L | Exact-accession provider medium Gram Amount: 30.00 g/L, preserving displayed precision. This is a source-reported rate or null, not an inferred stock dose or a normalization from the linked DSMZ recipe. Mol Amount and SEED aggregates are not adopted. | |
| 13 |
MES
C6H13NO4S 195.24 g/mol
|
1.95 G_PER_L |
CHEBI:39005
2-(N-morpholino)ethanesulfonic acid
|
Exact-accession provider medium Gram Amount: 1.95 g/L, preserving displayed precision. This is a source-reported rate or null, not an inferred stock dose or a normalization from the linked DSMZ recipe. Mol Amount and SEED aggregates are not adopted. |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 829a |
| 2026-02-03T11:19:18.909233 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 829 |
| 2026-03-13T03:09:42.325232Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:41.974109Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006565 |
| 2026-03-17T06:30:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:59.509657+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFUROBACTERIUM ATLANTICUM MEDIUM" to "desulfurobacterium_atlanticum_medium" for consistent matching |
| 2026-06-05T07:40:26.845662+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:46.030384+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-15T20:07:39.726500+00:00 | dedupe_identical_ingredients.py | REMOVED_DUPLICATE_INGREDIENT_ROWS | Removed 3 ingredient row(s) that were identical in every field to another row in this record, which made the recipe read as double-strength in those components (#263): Sodium chloride, Tryptone, Yeast extract. |
| 2026-09-02T07:37:39.757931+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |
| 2026-10-09T04:52:49.323535+00:00 | codex | RESTORED_MEDIUM_829_SOURCE_FORMULATIONS_AND_STOCK_SCOPES_WITH_EVIDENCE | Restore exact-source medium-829 formulations and stock boundaries, preserve provider rates/nulls and source conflicts, repair quantity units and family scope, and retain prior evidence/history. Each scientific change is linked to an inspected source excerpt; no growth association inferred. |