Overview
- External ontology
- komodo.medium:841 (source link unavailable) — MSV-THIOTRIX-MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.4
- Notes
- Source: KOMODO ModelSEED | ID: 841 | DSMZ Medium: 841 (mediadive.medium:841) | Aerobic: No
Composition
flowchart LR
CultureMech_006644["msv_thiotrix_medium"]
ing0_Agar["Agar"]
CultureMech_006644 --> ing0_Agar
CHEBI_2509["CHEBI:2509"]
ing0_Agar -.-> CHEBI_2509
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_006644 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006644 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_006644 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_K2HPO4["K2HPO4"]
CultureMech_006644 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_KH2PO4["KH2PO4"]
CultureMech_006644 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_006644 --> ing6_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing6_FeCl3_x_6_H2O -.-> CHEBI_86254
ing7_Na2_EDTA["Na2-EDTA"]
CultureMech_006644 --> ing7_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing7_Na2_EDTA -.-> CHEBI_64734
ing8_Na_acetate["Na-acetate"]
CultureMech_006644 --> ing8_Na_acetate
CHEBI_32954["CHEBI:32954"]
ing8_Na_acetate -.-> CHEBI_32954
ing9_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_006644 --> ing9_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing9_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing10_NaHCO3["NaHCO3"]
CultureMech_006644 --> ing10_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing10_NaHCO3 -.-> CHEBI_32139
ing11_Inositol["Inositol"]
CultureMech_006644 --> ing11_Inositol
CHEBI_24848["CHEBI:24848"]
ing11_Inositol -.-> CHEBI_24848
ing12_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_006644 --> ing12_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing12_Calcium_pantothenate -.-> CHEBI_31345
ing13_Biotin["Biotin"]
CultureMech_006644 --> ing13_Biotin
CHEBI_15956["CHEBI:15956"]
ing13_Biotin -.-> CHEBI_15956
ing14_Folic_acid["Folic acid"]
CultureMech_006644 --> ing14_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing14_Folic_acid -.-> CHEBI_27470
ing15_Vitamin_B12["Vitamin B12"]
CultureMech_006644 --> ing15_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing15_Vitamin_B12 -.-> CHEBI_176843
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.504541 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.100908 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0504541 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.110999 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.0857719 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.00201816 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 8 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.00302725 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 9 |
Na-acetate
C2H3O2.Na 82.034 g/mol
|
0.550959 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 10 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
0.403633 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 11 |
NaHCO3
CHO3.Na 84.006 g/mol
|
52.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 12 |
Inositol
C6H12O6 180.156 g/mol
|
100 G_PER_L |
CHEBI:24848
inositol
|
|
| 13 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 14 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.001 G_PER_L |
CHEBI:15956
biotin
|
|
| 15 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 16 | Vitamin B12 | 0.01 G_PER_L |
CHEBI:176843
vitamin B12
|
Solutions
Standard vitamin solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Riboflavin | 0.1 G_PER_L | |
| Thiamine-HCl x 2 H2O | 0.5 G_PER_L | |
| Nicotinic acid | 0.5 G_PER_L | |
| Pyridoxine hydrochloride | 0.5 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 841 |
| 2026-02-03T11:19:02.509141 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 841 |
| 2026-03-13T06:19:42.609877Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006644 |
| 2026-03-17T06:30:03.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:00.258116+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MSV-THIOTRIX-MEDIUM" to "msv_thiotrix_medium" for consistent matching |
| 2026-06-05T07:40:27.006645+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 20 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:46.085949+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-07T20:44:33.607152+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 4 stock-strength component(s) out of ingredients into 1 solution(s): 4 -> 'Standard vitamin solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |