Overview
- External ontology
- komodo.medium:845 (source link unavailable) — SPIRILLUM D427 medium
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.2, 'max': 7.4}
- Notes
- Source: KOMODO ModelSEED | ID: 845 | DSMZ Medium: 845 (mediadive.medium:845) | Aerobic: Yes
Composition
flowchart LR
CultureMech_006650["spirillum_d427_medium"]
ing0_Peptone["Peptone"]
CultureMech_006650 --> ing0_Peptone
ing1_Disodium_succinate["Disodium succinate"]
CultureMech_006650 --> ing1_Disodium_succinate
CHEBI_63675["CHEBI:63675"]
ing1_Disodium_succinate -.-> CHEBI_63675
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_006650 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006650 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_006650 --> ing4_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing4_FeCl3_x_6_H2O -.-> CHEBI_86254
ing5_MnSO4_x_H2O["MnSO4 x H2O"]
CultureMech_006650 --> ing5_MnSO4_x_H2O
CHEBI_86364["CHEBI:86364"]
ing5_MnSO4_x_H2O -.-> CHEBI_86364
ing6_Agar["Agar"]
CultureMech_006650 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_Catalase["Catalase"]
CultureMech_006650 --> ing7_Catalase
ing8_Na2_EDTA_x_2_H2O["Na2-EDTA x 2 H2O"]
CultureMech_006650 --> ing8_Na2_EDTA_x_2_H2O
CHEBI_64758["CHEBI:64758"]
ing8_Na2_EDTA_x_2_H2O -.-> CHEBI_64758
ing9_ZnCl2["ZnCl2"]
CultureMech_006650 --> ing9_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing9_ZnCl2 -.-> CHEBI_49976
ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_006650 --> ing10_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
ing11_H3BO3["H3BO3"]
CultureMech_006650 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_006650 --> ing12_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_006650 --> ing13_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_006650 --> ing14_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_006650 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing16_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_006650 --> ing16_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing16_p_Aminobenzoic_acid -.-> CHEBI_30753
ing17_D_____biotin["D-(+)-biotin"]
CultureMech_006650 --> ing17_D_____biotin
CHEBI_15956["CHEBI:15956"]
ing17_D_____biotin -.-> CHEBI_15956
ing18_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_006650 --> ing18_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing18_Calcium_pantothenate -.-> CHEBI_31345
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Peptone | 4.98504 G_PER_L | ||
| 2 |
Disodium succinate
C4H4O4.2Na 162.052 g/mol
|
0.997009 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
|
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.997009 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.997009 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.00199402 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 6 |
MnSO4 x H2O
H2O.Mn.O4S 169.016 g/mol
|
0.00199402 G_PER_L |
CHEBI:86364
manganese(II) sulfate monohydrate
|
|
| 7 | Agar | 0.997009 G_PER_L |
CHEBI:2509
agar
|
|
| 8 | Catalase | 1 G_PER_L | ||
| 9 |
Na2-EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
|
5.2 G_PER_L |
CHEBI:64758
EDTA disodium salt dihydrate
|
|
| 10 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 11 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 13 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 14 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 15 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 17 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.08 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 18 |
D-(+)-biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 19 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Solutions
Trace element solution SL-11
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| FeCl2 x 4 H2O | 1.5 G_PER_L |
Seven vitamins solution
Dose: 1 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Vitamin B12 | 0.1 G_PER_L | |
| Nicotinic acid | 0.2 G_PER_L | |
| Pyridoxine hydrochloride | 0.3 G_PER_L | |
| Thiamine-HCl x 2 H2O | 0.2 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable). |
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 845 |
| 2026-02-03T11:18:58.862130 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 845 |
| 2026-03-13T06:19:42.689803Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006650 |
| 2026-03-17T06:29:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:00.355295+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SPIRILLUM D427 medium" to "spirillum_d427_medium" for consistent matching |
| 2026-05-20T06:02:05.502888+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7.2-7.4' -> {'min': 7.2, 'max': 7.4} |
| 2026-06-05T07:41:46.196895+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.584713+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-15T00:17:31.718867+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 5 stock-strength component(s) out of ingredients into 2 solution(s): 1 -> 'Trace element solution SL-11' @ 1 ml/l; 4 -> 'Seven vitamins solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |
| 2026-08-15T05:34:58.530634+00:00 | promote_komodo_volume_candidates.py | ASSERTED_VOLUME_FROM_COMPOSITION_MATCH | This record reproduces MediaDive medium 845 ('SPIRILLUM WINOGRADSKYI MEDIUM') across 23 shared compounds at 100% identical values. That identifies the medium despite the name difference, so its stated addition volume is now asserted rather than proposed (#262). |