ae_medium

CultureMech:006656 bacterial COMPLEX SOLID_AGAR
Original name: AE medium

Overview

External ontology
komodo.medium:849 (source link unavailable) — AE medium
Applications
  • Microbial cultivation
Notes
Source: KOMODO ModelSEED | ID: 849 | DSMZ Medium: 849 (mediadive.medium:849) | Aerobic: No

Composition

flowchart LR
    CultureMech_006656["ae_medium"]
    ing0_Glucose["Glucose"]
    CultureMech_006656 --> ing0_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing0_Glucose -.-> CHEBI_17234
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_006656 --> ing1_Yeast_extract
    ing2_Peptone["Peptone"]
    CultureMech_006656 --> ing2_Peptone
    ing3_Absolute_ethanol["Absolute ethanol"]
    CultureMech_006656 --> ing3_Absolute_ethanol
    CHEBI_16236["CHEBI:16236"]
    ing3_Absolute_ethanol -.-> CHEBI_16236
    ing4_Glacial_acetic_acid["Glacial acetic acid"]
    CultureMech_006656 --> ing4_Glacial_acetic_acid
    CHEBI_15366["CHEBI:15366"]
    ing4_Glacial_acetic_acid -.-> CHEBI_15366
    ing5_Agar["Agar"]
    CultureMech_006656 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_Distilled_water["Distilled water"]
    CultureMech_006656 --> ing6_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing6_Distilled_water -.-> CHEBI_15377

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Glucose
C6H12O6 180.156 g/mol
5.0 G_PER_L CHEBI:17234
glucose
 DSMZ Medium 849 lists 5.0 g glucose.
2 Yeast extract 3.0 G_PER_L FOODON:03315426
yeast extract
 DSMZ Medium 849 lists 3.0 g yeast extract.
3 Peptone 4.0 G_PER_L MICRO:0000178 (source link unavailable)
peptone
 DSMZ Medium 849 lists 4.0 g peptone.
4 Absolute ethanol
C2H6O 46.069 g/mol
30.0 ML_PER_L CHEBI:16236
ethanol
 DSMZ Medium 849 lists 30.0 ml absolute ethanol.
5 Glacial acetic acid
C2H4O2 60.052 g/mol
30.0 ML_PER_L CHEBI:15366
acetic acid
 DSMZ Medium 849 lists 30.0 ml glacial acetic acid.
6 Agar 9.0 G_PER_L CHEBI:2509
agar
 DSMZ Medium 849 lists 9.0 g agar.
7 Distilled water
H2O 18.015 g/mol
940.0 ML_PER_L CHEBI:15377
water
 DSMZ Medium 849 lists 940.0 ml distilled water.

Preparation

  1. Autoclave the medium without the addition of glacial acetic acid and absolute ethanol.
  2. Sterilize glacial acetic acid and absolute ethanol by filtration through Teflon filters or by autoclaving in completely closed screw-cap glass bottles sealed with Teflon-coated septa.
  3. Add sterile glacial acetic acid and absolute ethanol.
  4. Incubate plates in sealed containers to keep humidity high above 90%.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:03.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 849
2026-02-03T11:19:03.159005 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 849
2026-03-13T06:19:42.755990Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:006656
2026-03-17T06:30:05.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:00.427889+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "AE medium" to "ae_medium" for consistent matching
2026-06-05T07:40:27.049495+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_dsmz_849_ae_score15.py RESOLVED_DSMZ_849_AE_SCORE15 DSMZ Medium 849 lists AE medium with glucose, yeast extract, peptone, 30.0 ml absolute ethanol, 30.0 ml glacial acetic acid, agar, and 940.0 ml distilled water.