Overview
- External ontology
- komodo.medium:868 (source link unavailable) — DESULFOVIGRA ADIPICA
- Applications
-
- Microbial cultivation
- pH range
- {'min': 7.0, 'max': 7.2}
- Notes
- Archived DSMZ Medium 868 lists Desulfovigra adipica as basal DSMZ Medium 503 supplemented per liter with 7.0 ml 10% Na2SO4, 10.0 ml 10% yeast extract, 1.0 ml trace element solution from DSMZ Medium 320, 50.0 ml 5% NaHCO3, 1.0 ml selenite-tungstate solution from DSMZ Medium 385, 10.0 ml vitamin solution from DSMZ Medium 141, 1.0 ml 10% propanol, and 17.0 ml 3% Na2S x 9H2O from sterile anaerobic stock solutions. The sheet states to adjust the completed medium to pH 7.0-7.2 with sterile anaerobic 5% Na2CO3 and to feed the same amount of propanol again after growth starts.
Ingredients (0)
No ingredients recorded.
Preparation
- Use basal DSMZ Medium 503 and add the Na2SO4, yeast extract, trace element, NaHCO3, selenite-tungstate, vitamin, propanol, and Na2S x 9H2O supplements from sterile anaerobic stock solutions at the listed amounts per liter.
- After completing the medium, adjust to pH 7.0-7.2 with sterile anaerobic 5% Na2CO3.
- When growth has started, feed the same amount of propanol again.
Solutions
10% (w/v) Na2SO4 solution
Dose: 7.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2SO4 | 100.0 G_PER_L | Solute concentration for DSMZ Medium 868 10% (w/v) Na2SO4 stock. |
DSMZ Medium 868 adds 7.0 ml/L sterile anaerobic 10% Na2SO4 stock.
10% (w/v) Yeast extract solution
Dose: 10.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Yeast extract | 100.0 G_PER_L | Solute concentration for DSMZ Medium 868 10% (w/v) yeast extract stock. |
DSMZ Medium 868 adds 10.0 ml/L sterile anaerobic 10% yeast extract stock.
Trace element solution SL-10 (DSMZ Medium 320)
Dose: 1.0 ML_PER_L
DSMZ Medium 868 adds 1.0 ml/L sterile anaerobic trace element solution from DSMZ Medium 320.
5% (w/v) NaHCO3 solution
Dose: 50.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaHCO3 | 50.0 G_PER_L | Solute concentration for DSMZ Medium 868 5% (w/v) NaHCO3 stock. |
DSMZ Medium 868 adds 50.0 ml/L sterile anaerobic 5% NaHCO3 stock.
Selenite-tungstate solution (DSMZ Medium 385)
Dose: 1.0 ML_PER_L
DSMZ Medium 868 adds 1.0 ml/L sterile anaerobic selenite-tungstate solution from DSMZ Medium 385.
Vitamin solution (DSMZ Medium 141)
Dose: 10.0 ML_PER_L
DSMZ Medium 868 adds 10.0 ml/L sterile anaerobic vitamin solution from DSMZ Medium 141.
10% (v/v) Propanol solution
Dose: 1.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Propanol | 10.0 PERCENT_V_V | Solute proportion for DSMZ Medium 868 10% (v/v) propanol stock. |
DSMZ Medium 868 adds 1.0 ml/L sterile anaerobic 10% propanol stock.
3% (w/v) Na2S x 9H2O solution
Dose: 17.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2S x 9H2O | 30.0 G_PER_L | Solute concentration for DSMZ Medium 868 3% (w/v) Na2S x 9H2O stock. |
DSMZ Medium 868 adds 17.0 ml/L sterile anaerobic 3% Na2S x 9H2O stock.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 868 |
| 2026-03-13T06:19:43.128330Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006698 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Na2CO3 |
| 2026-03-17T06:30:32.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:00.848121+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFOVIGRA ADIPICA" to "desulfovigra_adipica" for consistent matching |
| 2026-05-16T09:03:30.386464+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-05-20T06:01:15.032686+00:00 | migrate_ph_range.py | MIGRATED_PH_RANGE | ph_range '7-7.2' -> {'min': 7.0, 'max': 7.2} |
| 2026-06-05T07:40:58.063313+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:56.138480+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-11T00:00:00-07:00 | repair_dsmz_868_desulfovigra_adipica_score15.py | RESOLVED_DSMZ_868_DESULFOVIGRA_ADIPICA_SCORE15 | Archived DSMZ Medium 868 lists Desulfovigra adipica as basal DSMZ Medium 503 supplemented per liter with 7.0 ml 10% Na2SO4, 10.0 ml 10% yeast extract, 1.0 ml trace element solution from DSMZ Medium 320, 50.0 ml 5% NaHCO3, 1.0 ml selenite-tungstate solution from DSMZ Medium 385, 10.0 ml vitamin solution from DSMZ Medium 141, 1.0 ml 10% propanol, and 17.0 ml 3% Na2S x 9H2O from sterile anaerobic stock solutions. The sheet states to adjust the completed medium to pH 7.0-7.2 with sterile anaerobic 5% Na2CO3 and to feed the same amount of propanol again after growth starts. Replaced the KOMODO pH-buffer placeholder with the archived DSMZ supplement list and removed a stale kg_microbe_match to MediaDive 634c. |