Overview
- External ontology
- komodo.medium:87a_13941 (source link unavailable) — MEDIUM 87a MODIFIED FOR DSM 13941
- Applications
-
- Microbial cultivation
- pH
- 8.2
- Notes
- Source: KOMODO ModelSEED | ID: 87a_13941 | DSMZ Medium: 87a (mediadive.medium:87a) | Aerobic: No
Composition
flowchart LR
CultureMech_006720["medium_87a_modified_for_dsm_13941"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_006720 --> ing0_Yeast_extract
ing1_Glycyl_glycine["Glycyl-glycine"]
CultureMech_006720 --> ing1_Glycyl_glycine
CHEBI_17201["CHEBI:17201"]
ing1_Glycyl_glycine -.-> CHEBI_17201
ing2_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
CultureMech_006720 --> ing2_Na2HPO4_x_2_H2O
CHEBI_91258["CHEBI:91258"]
ing2_Na2HPO4_x_2_H2O -.-> CHEBI_91258
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006720 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_KNO3["KNO3"]
CultureMech_006720 --> ing4_KNO3
CHEBI_63043["CHEBI:63043"]
ing4_KNO3 -.-> CHEBI_63043
ing5_NaNO3["NaNO3"]
CultureMech_006720 --> ing5_NaNO3
CHEBI_63005["CHEBI:63005"]
ing5_NaNO3 -.-> CHEBI_63005
ing6_NaCl["NaCl"]
CultureMech_006720 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_006720 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8_Fe_III__citrate["Fe(III) citrate"]
CultureMech_006720 --> ing8_Fe_III__citrate
CHEBI_144421["CHEBI:144421"]
ing8_Fe_III__citrate -.-> CHEBI_144421
ing9_Biotin["Biotin"]
CultureMech_006720 --> ing9_Biotin
CHEBI_15956["CHEBI:15956"]
ing9_Biotin -.-> CHEBI_15956
ing10_Vitamin_B12["Vitamin B12"]
CultureMech_006720 --> ing10_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing10_Vitamin_B12 -.-> CHEBI_176843
ing11_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_006720 --> ing11_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing11_Calcium_pantothenate -.-> CHEBI_31345
ing12_Na3_EDTA["Na3-EDTA"]
CultureMech_006720 --> ing12_Na3_EDTA
CHEBI_63125["CHEBI:63125"]
ing12_Na3_EDTA -.-> CHEBI_63125
ing13_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_006720 --> ing13_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing13_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_006720 --> ing14_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
ing15_H3BO3["H3BO3"]
CultureMech_006720 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_006720 --> ing16_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_006720 --> ing17_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
ing18_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_006720 --> ing18_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing18_NiCl2_x_6_H2O -.-> CHEBI_34887
ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_006720 --> ing19_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing20_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_006720 --> ing20_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing20_Na2S_x_9_H2O -.-> CHEBI_76209
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.952381 G_PER_L | ||
| 2 |
Glycyl-glycine
C4H8N2O3 132.119 g/mol
|
0.952381 G_PER_L |
CHEBI:17201
glycylglycine
|
|
| 3 |
Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
|
0.0952381 G_PER_L |
CHEBI:91258
disodium hydrogenphosphate dihydrate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.0952381 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
KNO3
K.NO3 101.102 g/mol
|
0.0952381 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 6 |
NaNO3
NO3.Na 84.994 g/mol
|
0.47619 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
0.0952381 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.047619 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 9 |
Fe(III) citrate
C6H5FeO7 244.944 g/mol
|
5 G_PER_L |
CHEBI:144421
iron(III) citrate
|
|
| 10 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.05 G_PER_L |
CHEBI:15956
biotin
|
|
| 11 | Vitamin B12 | 0.01 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 12 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 13 |
Na3-EDTA
C10H13N2O8.3Na 358.19 g/mol
|
2 G_PER_L |
CHEBI:63125
EDTA trisodium salt
|
|
| 14 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 15 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 17 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 18 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 19 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 20 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 21 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
30 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Solutions
Vitamin solution CA
| Ingredient | Concentration | Notes |
|---|---|---|
| Nicotinic acid | 1 G_PER_L | |
| Thiamine-HCl x 2 H2O | 1 G_PER_L | |
| p-Aminobenzoic acid | 0.5 G_PER_L | |
| Pyridoxine hydrochloride | 0.5 G_PER_L | |
| Folic acid | 0.5 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 87a_13941 |
| 2026-02-03T11:19:01.662878 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 87 |
| 2026-03-13T06:19:43.337373Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006720 |
| 2026-03-17T06:30:00.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:01.079196+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MEDIUM 87a MODIFIED FOR DSM 13941" to "medium_87a_modified_for_dsm_13941" for consistent matching |
| 2026-06-05T07:41:28.171114+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.381812+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:05.829721+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-15T00:17:31.652854+00:00 | apply_cocktail_nesting.py | NESTED_FLATTENED_COCKTAIL | Moved 5 stock-strength component(s) out of ingredients into 1 solution(s): 5 -> 'Vitamin solution CA' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150). |