methylophaga_sulfidovorans_medium

CultureMech:006894 bacterial DEFINED SOLID_AGAR
Original name: METHYLOPHAGA SULFIDOVORANS MEDIUM

Overview

External ontology
komodo.medium:951 (source link unavailable) — METHYLOPHAGA SULFIDOVORANS MEDIUM
Applications
  • Microbial cultivation
pH range
{'min': 7.2, 'max': 7.8}
Notes
pH buffer: HCl | Source: KOMODO ModelSEED | ID: 951 | DSMZ Medium: 951 (mediadive.medium:951) | Aerobic: No

Composition

flowchart LR
    CultureMech_006894["methylophaga_sulfidovorans_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_006894 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1__NH4_2SO4["(NH4)2SO4"]
    CultureMech_006894 --> ing1__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing1__NH4_2SO4 -.-> CHEBI_62946
    ing2_CaCl2_x_6_H2O["CaCl2 x 6 H2O"]
    CultureMech_006894 --> ing2_CaCl2_x_6_H2O
    CHEBI_91243["CHEBI:91243"]
    ing2_CaCl2_x_6_H2O -.-> CHEBI_91243
    ing3_KCl["KCl"]
    CultureMech_006894 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_006894 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_KH2PO4["KH2PO4"]
    CultureMech_006894 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_Na2CO3["Na2CO3"]
    CultureMech_006894 --> ing6_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing6_Na2CO3 -.-> CHEBI_29377
    ing7_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_006894 --> ing7_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing7_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing8_Methanol["Methanol"]
    CultureMech_006894 --> ing8_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing8_Methanol -.-> CHEBI_17790
    ing9_Agar["Agar"]
    CultureMech_006894 --> ing9_Agar
    CHEBI_2509["CHEBI:2509"]
    ing9_Agar -.-> CHEBI_2509
    ing10_HCl["HCl"]
    CultureMech_006894 --> ing10_HCl
    CHEBI_17883["CHEBI:17883"]
    ing10_HCl -.-> CHEBI_17883
    ing11_ZnCl2["ZnCl2"]
    CultureMech_006894 --> ing11_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing11_ZnCl2 -.-> CHEBI_49976
    ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_006894 --> ing12_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing13_H3BO3["H3BO3"]
    CultureMech_006894 --> ing13_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing13_H3BO3 -.-> CHEBI_33118
    ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_006894 --> ing14_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_006894 --> ing15_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_006894 --> ing16_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_006894 --> ing17_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing18_Biotin["Biotin"]
    CultureMech_006894 --> ing18_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing18_Biotin -.-> CHEBI_15956
    ing19_Pantothenate["Pantothenate"]
    CultureMech_006894 --> ing19_Pantothenate
    CHEBI_16454["CHEBI:16454"]
    ing19_Pantothenate -.-> CHEBI_16454
    ing20_Riboflavine["Riboflavine"]
    CultureMech_006894 --> ing20_Riboflavine
    CHEBI_17015["CHEBI:17015"]
    ing20_Riboflavine -.-> CHEBI_17015
    ing21_Vitamin_B12["Vitamin B12"]
    CultureMech_006894 --> ing21_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing21_Vitamin_B12 -.-> CHEBI_176843

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
15 G_PER_L CHEBI:26710
sodium chloride
2 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.5 G_PER_L CHEBI:62946
ammonium sulfate
3 CaCl2 x 6 H2O
Ca.2Cl.6H2O 219.074 g/mol
0.33 G_PER_L CHEBI:91243
calcium chloride hexahydrate
4 KCl
Cl.K 74.551 g/mol
0.2 G_PER_L CHEBI:32588
potassium chloride
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 KH2PO4
H2O4P.K 136.084 g/mol
0.02 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
7 Na2CO3
CO3.2Na 105.988 g/mol
2 G_PER_L CHEBI:29377
sodium carbonate
8 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.001 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
9 Methanol
CH4O 32.042 g/mol
7.92 G_PER_L CHEBI:17790
methanol
10 Agar 15 G_PER_L CHEBI:2509
agar
11 HCl
HCl 36.461 g/mol
5 G_PER_L CHEBI:17883
hydrogen chloride
12 ZnCl2
2Cl.Zn 136.296 g/mol
0.0696517 G_PER_L CHEBI:49976
zinc dichloride
13 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.199005 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
14 H3BO3
H3BO3 61.833 g/mol
0.00597015 G_PER_L CHEBI:33118
boric acid
15 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.189055 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
16 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.00199005 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
17 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.0238806 G_PER_L CHEBI:34887
nickel dichloride
18 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.0358209 G_PER_L CHEBI:75213
sodium molybdate dihydrate
19 Biotin
C10H16N2O3S 244.316 g/mol
0.02 G_PER_L CHEBI:15956
biotin
20 Pantothenate
C9H16NO5 218.229 g/mol
0.05 G_PER_L CHEBI:16454
pantothenate
21 Riboflavine
C17H20N4O6 376.369 g/mol
0.01 G_PER_L CHEBI:17015
riboflavin
22 Vitamin B12 0.01 G_PER_L CHEBI:176843
vitamin B12

Solutions

Trace element solution SL10

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeCl2 x 4 H2O 1.49254 G_PER_L

Vitamin solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
Nicotinic acid 0.2 G_PER_L
Thiamine 0.1 G_PER_L
p-Aminobenzoic acid 0.1 G_PER_L
Pyridoxine hydrochloride 0.5 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:03.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 951
2026-02-03T11:18:58.148754 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 951
2026-03-13T06:19:44.726534Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:006894
2026-03-17T06:29:50.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:02.699080+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHYLOPHAGA SULFIDOVORANS MEDIUM" to "methylophaga_sulfidovorans_medium" for consistent matching
2026-05-20T06:00:55.086918+00:00 migrate_ph_range.py MIGRATED_PH_RANGE ph_range '7.2-7.8' -> {'min': 7.2, 'max': 7.8}
2026-06-05T07:40:28.125059+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:46.507152+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T20:44:33.760388+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 5 stock-strength component(s) out of ingredients into 2 solution(s): 1 -> 'Trace element solution SL10' @ 1 ml/l; 4 -> 'Vitamin solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).