medium_for_h_dombrowskii (CultureMech:006897)

Overview

External ontology

komodo.medium:954 (source link unavailable) — medium FOR H. DOMBROWSKII

Applications

Microbial cultivation

pH

7.4

High-metal flag

True

Notes

Source: KOMODO ModelSEED | ID: 954 | DSMZ Medium: 954 (mediadive.medium:954) | Aerobic: No

Composition

flowchart LR
    CultureMech_006897["medium_for_h_dombrowskii"]
    ing0_Casamino_acids["Casamino acids"]
    CultureMech_006897 --> ing0_Casamino_acids
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_006897 --> ing1_Yeast_extract
    ing2_Tris["Tris"]
    CultureMech_006897 --> ing2_Tris
    CHEBI_9754["CHEBI:9754"]
    ing2_Tris -.-> CHEBI_9754
    ing3_KCl["KCl"]
    CultureMech_006897 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_006897 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_006897 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_NaCl["NaCl"]
    CultureMech_006897 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_Agar["Agar"]
    CultureMech_006897 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509

Ingredients (8)

#	Ingredient	Concentration	Ontology
1	Casamino acids	5 G_PER_L
2	Yeast extract	5 G_PER_L
3	Tris C4H11NO3 121.136 g/mol	12.1 G_PER_L	CHEBI:9754 tris
4	KCl Cl.K 74.551 g/mol	2 G_PER_L	CHEBI:32588 potassium chloride
5	MgCl2 x 6 H2O 2Cl.6H2O.Mg 203.301 g/mol	20 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate
6	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol	0.2 G_PER_L	CHEBI:86158 calcium chloride dihydrate
7	NaCl Cl.Na 58.443 g/mol	200 G_PER_L	CHEBI:26710 sodium chloride
8	Agar	20 G_PER_L	CHEBI:2509 agar

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:03.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 954
2026-02-03T11:19:08.457738	dsmz-resolver-v1.0	Enriched with DSMZ composition	Copied ingredients from DSMZ Medium 954
2026-03-13T06:19:44.739191Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:006897
2026-03-17T06:30:21.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:02.714585+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "medium FOR H. DOMBROWSKII" to "medium_for_h_dombrowskii" for consistent matching
2026-06-05T07:40:28.144076+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:28.800819+00:00	chebi-regrounding-v1.0	Corrected CHEBI ingredient grounding (audit G21/G22)	Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-10-08T06:29:49.318246+00:00	codex	CURATED_KOMODO_CONCENTRATION_SOURCE_EVIDENCE	Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. KOMODO accession 954 is mapped to the same DSMZ accession by https://komodo.modelseed.org/servlet/KomodoTomcatServerSideUtilitiesModelSeed?MediaList; mapping response SHA256=d8259d98e6dbffdf6961a1ab92bc86f3567d5d96de352bfa079edb2a91e9580a. The mapping supports identity only, not ingredient quantities or variants.