medium_for_dsm_14457_and_14458_replace_methanol_with_methylamine

CultureMech:006899 bacterial DEFINED LIQUID
Original name: MEDIUM FOR DSM 14457 AND 14458_replace_Methanol_with_Methylamine

Overview

External ontology
komodo.medium:956_replace_Methanol_with_Methylamine (source link unavailable) — MEDIUM FOR DSM 14457 AND 14458_replace_Methanol_with_Methylamine
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: KOMODO ModelSEED | ID: 956_replace_Methanol_with_Methylamine | DSMZ Medium: 956 (mediadive.medium:956) | Aerobic: No

Composition

flowchart LR
    CultureMech_006899["medium_for_dsm_14457_and_14458_replace_methanol_with_methylamine"]
    ing0_Methylamine["Methylamine"]
    CultureMech_006899 --> ing0_Methylamine
    CHEBI_16830["CHEBI:16830"]
    ing0_Methylamine -.-> CHEBI_16830

Ingredients (1)

# Ingredient Concentration Ontology Notes
1 Methylamine
CH5N 31.058 g/mol
CHEBI:16830
methylamine
 After sterilisation add sterile Methylamine to the source-stated final concentration of 0.3%. The percentage basis, supplement dose volume, and final receiving volume are unspecified. No mass concentration, density, or percent unit is inferred. This source-specific variant selects methylamine and excludes methanol.

Preparation

  1. pH 7.2. After sterilising the base, add sterile methylamine to a final concentration of 0.3% (percentage basis unspecified). Do not add methanol in this variant.

Solutions

DSMZ 956 base preparation

Stock composition
IngredientConcentrationNotes
KH2PO4 Source batch input: 2.000 g; KH2PO4 2.000 g The recipe starts with 1000 ml distilled water before supplementation. No prepared-base or final-medium mass concentration is asserted.
(NH4)2SO4 Source batch input: 2.000 g; (NH4)2SO4 2.000 g The recipe starts with 1000 ml distilled water before supplementation. No prepared-base or final-medium mass concentration is asserted.
MgSO4 x 7 H2O Source batch input: 0.125 g; MgSO4 x 7 H2O 0.125 g The recipe starts with 1000 ml distilled water before supplementation. No prepared-base or final-medium mass concentration is asserted.
NaCl Source batch input: 0.500 g; NaCl 0.500 g The recipe starts with 1000 ml distilled water before supplementation. No prepared-base or final-medium mass concentration is asserted.
FeSO4 x 7 H2O Source batch input: 0.002 g; FeSO4 x 7 H2O 0.002 g The recipe starts with 1000 ml distilled water before supplementation. No prepared-base or final-medium mass concentration is asserted.
Distilled water 1 L Starting-water batch amount: 1000 ml / 1000 ml per L = 1 L. Not a final-volume make-up instruction or a mass concentration.
Curator-described preparation stage, not a separately accessioned stock. Composition records source batch inputs. The 1000 ml water is an input, not an explicit prepared-base or final-medium volume; no absolute base dose is inferred.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:03.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 956_replace_Methanol_with_Methylamine
2026-02-03T11:19:17.168002 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 956
2026-03-13T06:19:44.747577Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:006899
2026-03-17T06:30:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:02.723941+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MEDIUM FOR DSM 14457 AND 14458_replace_Methanol_with_Methylamine" to "medium_for_dsm_14457_and_14458_replace_methanol_with_methylamine" for consistent matching
2026-06-05T07:41:29.017540+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:06.041795+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-10-07T16:26:13.553900+00:00 codex CORRECTED_DSMZ618_956_BATCH_INPUTS_AND_PERCENT_ALTERNATIVES Original PDF, preparation and exact source accession verified. Restored batch-input scope; withdrew unsupported final mass concentrations and preserved source amounts/percentages without guessing bases or final volume. Existing source-specific methylamine record reused. Prior history, IDs and unrelated metadata preserved; source-specific removed nodes remain explicit in this change event.