Overview
- External ontology
- komodo.medium:976 (source link unavailable) — METHYLOPHAGA ALCALICA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 9.5
- Notes
- Source: KOMODO ModelSEED | ID: 976 | DSMZ Medium: 976 (mediadive.medium:976) | Aerobic: No
Composition
flowchart LR
CultureMech_006934["methylophaga_alcalica_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_006934 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_KNO3["KNO3"]
CultureMech_006934 --> ing1_KNO3
CHEBI_63043["CHEBI:63043"]
ing1_KNO3 -.-> CHEBI_63043
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_006934 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_NaCl["NaCl"]
CultureMech_006934 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_Na2CO3["Na2CO3"]
CultureMech_006934 --> ing4_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing4_Na2CO3 -.-> CHEBI_29377
ing5_Methanol["Methanol"]
CultureMech_006934 --> ing5_Methanol
CHEBI_17790["CHEBI:17790"]
ing5_Methanol -.-> CHEBI_17790
ing6_Ferric_citrate["Ferric citrate"]
CultureMech_006934 --> ing6_Ferric_citrate
CHEBI_144421["CHEBI:144421"]
ing6_Ferric_citrate -.-> CHEBI_144421
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_006934 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
ing8_MgCl2_x_4_H2O["MgCl2 x 4 H2O"]
CultureMech_006934 --> ing8_MgCl2_x_4_H2O
ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_006934 --> ing9_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing10_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_006934 --> ing10_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing10_CuSO4_x_5_H2O -.-> CHEBI_31440
ing11_Agar["Agar"]
CultureMech_006934 --> ing11_Agar
CHEBI_2509["CHEBI:2509"]
ing11_Agar -.-> CHEBI_2509
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
KNO3
K.NO3 101.102 g/mol
|
1 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.22 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
Na2CO3
CO3.2Na 105.988 g/mol
|
5 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 6 |
Methanol
CH4O 32.042 g/mol
|
7.92 G_PER_L |
CHEBI:17790
methanol
|
|
| 7 |
Ferric citrate
C6H5FeO7 244.944 g/mol
|
0.03 G_PER_L |
CHEBI:144421
iron(III) citrate
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.03 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 9 | MgCl2 x 4 H2O | 0.005 G_PER_L | ||
| 10 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.005 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 11 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.5 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 12 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:03.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 976 |
| 2026-02-03T11:19:04.529803 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 976 |
| 2026-03-13T06:19:45.095591Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:006934 |
| 2026-03-17T06:30:09.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:03.116141+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHYLOPHAGA ALCALICA MEDIUM" to "methylophaga_alcalica_medium" for consistent matching |
| 2026-06-05T07:40:28.326035+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.412276+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:46.576768+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |