halobacterium_medium (CultureMech:006940)

Overview

External ontology

komodo.medium:97 (source link unavailable) — HALOBACTERIUM medium

Applications

Microbial cultivation

pH

7.4

High-metal flag

True

Notes

Source: KOMODO ModelSEED | ID: 97 | DSMZ Medium: 97 (mediadive.medium:97) | Aerobic: No

Composition

flowchart LR
    CultureMech_006940["halobacterium_medium"]
    ing0_Casamino_acids["Casamino acids"]
    CultureMech_006940 --> ing0_Casamino_acids
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_006940 --> ing1_Yeast_extract
    ing2_Na3_citrate["Na3-citrate"]
    CultureMech_006940 --> ing2_Na3_citrate
    CHEBI_53258["CHEBI:53258"]
    ing2_Na3_citrate -.-> CHEBI_53258
    ing3_KCl["KCl"]
    CultureMech_006940 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_006940 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_006940 --> ing5_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing6_MnSO4_x_H2O["MnSO4 x H2O"]
    CultureMech_006940 --> ing6_MnSO4_x_H2O
    CHEBI_86364["CHEBI:86364"]
    ing6_MnSO4_x_H2O -.-> CHEBI_86364
    ing7_NaCl["NaCl"]
    CultureMech_006940 --> ing7_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing7_NaCl -.-> CHEBI_26710
    ing8_Agar["Agar"]
    CultureMech_006940 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509

Ingredients (9)

#	Ingredient	Concentration	Ontology
1	Casamino acids	7.5 G_PER_L
2	Yeast extract	10 G_PER_L
3	Na3-citrate C6H5O7.3Na 258.069 g/mol	3 G_PER_L	CHEBI:53258 sodium citrate
4	KCl Cl.K 74.551 g/mol	2 G_PER_L	CHEBI:32588 potassium chloride
5	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol	20 G_PER_L	CHEBI:31795 magnesium sulfate heptahydrate
6	FeSO4 x 7 H2O Fe.7H2O.O4S 278.013 g/mol	0.05 G_PER_L	CHEBI:75836 iron(2+) sulfate heptahydrate
7	MnSO4 x H2O H2O.Mn.O4S 169.016 g/mol	0.0002 G_PER_L	CHEBI:86364 manganese(II) sulfate monohydrate
8	NaCl Cl.Na 58.443 g/mol	250 G_PER_L	CHEBI:26710 sodium chloride
9	Agar	20 G_PER_L	CHEBI:2509 agar

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T01:15:03.fZ	komodo-web-import	Imported from KOMODO web table	Source: KOMODO, ID: 97
2026-02-03T11:18:55.481578	dsmz-resolver-v1.0	Enriched with DSMZ composition	Copied ingredients from DSMZ Medium 97
2026-03-13T06:19:45.166460Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:006940
2026-03-17T06:29:42.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:03.196397+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "HALOBACTERIUM medium" to "halobacterium_medium" for consistent matching
2026-06-05T07:40:28.363697+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:28.822066+00:00	chebi-regrounding-v1.0	Corrected CHEBI ingredient grounding (audit G21/G22)	Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-10-07T21:02:15.484988+00:00	codex	CURATED_KOMODO_CONCENTRATION_SOURCE_EVIDENCE	Exact medium/source membership, ingredient inventory/forms, physical-state conditions, literal snippets, and arithmetic checked. Stock boundaries, source-only rows, preparation context, and variants remain explicit follow-up tasks; secondary chemical mappings unchanged. KOMODO accession 97 is mapped to the same DSMZ accession by https://komodo.modelseed.org/servlet/KomodoTomcatServerSideUtilitiesModelSeed?MediaList; mapping response SHA256=d8259d98e6dbffdf6961a1ab92bc86f3567d5d96de352bfa079edb2a91e9580a. The mapping supports identity only, not ingredient quantities or variants.