modified_isp5_medium

CultureMech:006975 bacterial COMPLEX SOLID_AGAR
Original name: MODIFIED ISP5 medium

Overview

External ontology
komodo.medium:993b (source link unavailable) — MODIFIED ISP5 medium
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: KOMODO ModelSEED | ID: 993b | DSMZ Medium: 993b (mediadive.medium:993b) | Aerobic: No

Composition

flowchart LR
    CultureMech_006975["modified_isp5_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_006975 --> ing0_Yeast_extract
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_006975 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_L_Asparagine["L-Asparagine"]
    CultureMech_006975 --> ing2_L_Asparagine
    CHEBI_17196["CHEBI:17196"]
    ing2_L_Asparagine -.-> CHEBI_17196
    ing3_Glycerol["Glycerol"]
    CultureMech_006975 --> ing3_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing3_Glycerol -.-> CHEBI_17754
    ing4_K2HPO4["K2HPO4"]
    CultureMech_006975 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_Agar["Agar"]
    CultureMech_006975 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Yeast extract 5 G_PER_L
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
100 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 L-Asparagine
C4H8N2O3 132.119 g/mol
1 G_PER_L CHEBI:17196
L-asparagine
4 Glycerol
C3H8O3 92.094 g/mol
10 G_PER_L CHEBI:17754
glycerol
5 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
6 Agar 20 G_PER_L CHEBI:2509
agar

Solutions

Trace element solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
FeSO4 x 7 H2O 1 G_PER_L
MnCl2 x 4 H2O 1 G_PER_L
ZnSO4 x 7 H2O 1 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T01:15:03.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 993b
2026-02-03T11:19:14.639770 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 993
2026-03-13T06:19:45.359124Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:006975
2026-03-17T06:30:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:03.415406+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED ISP5 medium" to "modified_isp5_medium" for consistent matching
2026-06-05T07:40:28.459475+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:46.624797+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-07T20:44:33.812293+00:00 apply_cocktail_nesting.py NESTED_FLATTENED_COCKTAIL Moved 3 stock-strength component(s) out of ingredients into 1 solution(s): 3 -> 'Trace element solution' @ 1 ml/l. Each moved component matched the MediaDive stock by name AND value; bulk ingredients sharing a name were left in place (#150).