mops_minimal_medium_bergholz_et_al

CultureMech:006989 bacterial DEFINED LIQUID
Original name: MOPS minimal medium (Bergholz et al)

Overview

External ontology
MEDIADB:102 (source link unavailable) — MOPS minimal medium (Bergholz et al)
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_006989["mops_minimal_medium_bergholz_et_al"]
    ing0_D_Glucose["D-Glucose"]
    CultureMech_006989 --> ing0_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing0_D_Glucose -.-> CHEBI_17634
    ing1_Magnesium_chloride["Magnesium chloride"]
    CultureMech_006989 --> ing1_Magnesium_chloride
    CHEBI_86345["CHEBI:86345"]
    ing1_Magnesium_chloride -.-> CHEBI_86345
    ing2_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_006989 --> ing2_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing2_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing3_Boric_acid["Boric acid"]
    CultureMech_006989 --> ing3_Boric_acid
    CHEBI_33118["CHEBI:33118"]
    ing3_Boric_acid -.-> CHEBI_33118
    ing4_Potassium_sulfate["Potassium sulfate"]
    CultureMech_006989 --> ing4_Potassium_sulfate
    CHEBI_32036["CHEBI:32036"]
    ing4_Potassium_sulfate -.-> CHEBI_32036
    ing5_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
    CultureMech_006989 --> ing5_Potassium_dibasic_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing5_Potassium_dibasic_phosphate -.-> CHEBI_131527
    ing6_Sodium_chloride["Sodium chloride"]
    CultureMech_006989 --> ing6_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing6_Sodium_chloride -.-> CHEBI_26710
    ing7_Ammonium_chloride["Ammonium chloride"]
    CultureMech_006989 --> ing7_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing7_Ammonium_chloride -.-> CHEBI_31206
    ing8_Ferrous_sulfate["Ferrous sulfate"]
    CultureMech_006989 --> ing8_Ferrous_sulfate
    CHEBI_75832["CHEBI:75832"]
    ing8_Ferrous_sulfate -.-> CHEBI_75832
    ing9_Cobalt_chloride["Cobalt chloride"]
    CultureMech_006989 --> ing9_Cobalt_chloride
    CHEBI_35696["CHEBI:35696"]
    ing9_Cobalt_chloride -.-> CHEBI_35696
    ing10_Zinc_sulfate["Zinc sulfate"]
    CultureMech_006989 --> ing10_Zinc_sulfate
    CHEBI_35176["CHEBI:35176"]
    ing10_Zinc_sulfate -.-> CHEBI_35176
    ing11_Manganese_chloride["Manganese chloride"]
    CultureMech_006989 --> ing11_Manganese_chloride
    CHEBI_63041["CHEBI:63041"]
    ing11_Manganese_chloride -.-> CHEBI_63041
    ing12_Cupric_sulfate["Cupric sulfate"]
    CultureMech_006989 --> ing12_Cupric_sulfate
    CHEBI_23414["CHEBI:23414"]
    ing12_Cupric_sulfate -.-> CHEBI_23414
    ing13_Molybdic_acid_ammonium_salt_tetrahydrate["Molybdic acid ammonium salt tetrahydrate"]
    CultureMech_006989 --> ing13_Molybdic_acid_ammonium_salt_tetrahydrate
    CHEBI_91249["CHEBI:91249"]
    ing13_Molybdic_acid_ammonium_salt_tetrahydrate -.-> CHEBI_91249
    ing14_MOPS["MOPS"]
    CultureMech_006989 --> ing14_MOPS
    CHEBI_39074["CHEBI:39074"]
    ing14_MOPS -.-> CHEBI_39074
    ing15_Tricine["Tricine"]
    CultureMech_006989 --> ing15_Tricine
    CHEBI_46760["CHEBI:46760"]
    ing15_Tricine -.-> CHEBI_46760

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 D-Glucose
C6H12O6 180.156 g/mol
5.5506 MILLIMOLAR CHEBI:17634
D-glucose
 Cross-references: KEGG:glc_D
2 Magnesium chloride
2Cl.6H2O.Mg 203.301 g/mol
0.523 MILLIMOLAR CHEBI:86345
magnesium dichloride hexahydrate
3 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.0005 MILLIMOLAR CHEBI:3312
calcium dichloride
4 Boric acid
H3BO3 61.833 g/mol
0.008 MILLIMOLAR CHEBI:33118
boric acid
5 Potassium sulfate
2K.O4S 174.259 g/mol
0.276 MILLIMOLAR CHEBI:32036
potassium sulfate
6 Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
2.64 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
7 Sodium chloride
Cl.Na 58.443 g/mol
50.0 MILLIMOLAR CHEBI:26710
sodium chloride
8 Ammonium chloride
Cl.H4N 53.492 g/mol
9.52 MILLIMOLAR CHEBI:31206
ammonium chloride
9 Ferrous sulfate
Fe.O4S 151.908 g/mol
0.01 MILLIMOLAR CHEBI:75832
iron(2+) sulfate (anhydrous)
10 Cobalt chloride
2Cl.Co 129.839 g/mol
6e-05 MILLIMOLAR CHEBI:35696
cobalt dichloride
11 Zinc sulfate
O4S.Zn 161.453 g/mol
0.0002 MILLIMOLAR CHEBI:35176
zinc sulfate
12 Manganese chloride
Cl2Mn 125.844 g/mol
0.0016 MILLIMOLAR CHEBI:63041
manganese(II) chloride
13 Cupric sulfate
Cu.O4S 159.609 g/mol
0.0002 MILLIMOLAR CHEBI:23414
copper(II) sulfate
14 Molybdic acid ammonium salt tetrahydrate
2H4N.MoO4 196.014 g/mol
6e-05 MILLIMOLAR CHEBI:91249
ammonium molybdate
15 MOPS 40.0 MILLIMOLAR CHEBI:39074
MOPS
16 Tricine 4.0 MILLIMOLAR CHEBI:46760
tricine

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:43.530190Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 102, Reference: Mazumdar et al. (2014) PLOS One
2026-01-28T19:48:34.387429Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:45.469220Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:006989
2026-03-17T06:30:38.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:03.544545+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "'MOPS minimal medium (Bergholz et al" to "mops_minimal_medium_bergholz_et_al" for consistent matching
2026-06-05T07:41:38.258541+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-31T06:18:18.038653+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored the medium's own name in media_term.term.label, original_name. The MediaDB SQL parser truncated every value containing a parenthesis at that parenthesis; recovered from media_database.07Oct2015.sql.