m9_seo

CultureMech:006991 bacterial DEFINED LIQUID
Original name: M9 (Seo)

Overview

External ontology
MEDIADB:104 (source link unavailable) — M9 (Seo)
Applications
  • Cultivation of genome-sequenced organisms
  • Metabolic modeling
  • Systems biology research
Notes
Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/

Composition

flowchart LR
    CultureMech_006991["m9_seo"]
    ing0_D_Glucose["D-Glucose"]
    CultureMech_006991 --> ing0_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing0_D_Glucose -.-> CHEBI_17634
    ing1_L_Proline["L-Proline"]
    CultureMech_006991 --> ing1_L_Proline
    CHEBI_17203["CHEBI:17203"]
    ing1_L_Proline -.-> CHEBI_17203
    ing2_Calcium_chloride_anhydrous["Calcium chloride anhydrous"]
    CultureMech_006991 --> ing2_Calcium_chloride_anhydrous
    CHEBI_3312["CHEBI:3312"]
    ing2_Calcium_chloride_anhydrous -.-> CHEBI_3312
    ing3_Dibasic_sodium_phosphate["Dibasic sodium phosphate"]
    CultureMech_006991 --> ing3_Dibasic_sodium_phosphate
    CHEBI_34683["CHEBI:34683"]
    ing3_Dibasic_sodium_phosphate -.-> CHEBI_34683
    ing4_Leucine["Leucine"]
    CultureMech_006991 --> ing4_Leucine
    CHEBI_15603["CHEBI:15603"]
    ing4_Leucine -.-> CHEBI_15603
    ing5_Sodium_chloride["Sodium chloride"]
    CultureMech_006991 --> ing5_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing5_Sodium_chloride -.-> CHEBI_26710
    ing6_Thiamine_HCl["Thiamine HCl"]
    CultureMech_006991 --> ing6_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing6_Thiamine_HCl -.-> CHEBI_49105
    ing7_Potassium_dihydrogen_phosphate["Potassium dihydrogen phosphate"]
    CultureMech_006991 --> ing7_Potassium_dihydrogen_phosphate
    CHEBI_63036["CHEBI:63036"]
    ing7_Potassium_dihydrogen_phosphate -.-> CHEBI_63036
    ing8_Magnesium_sulfate["Magnesium sulfate"]
    CultureMech_006991 --> ing8_Magnesium_sulfate
    CHEBI_32599["CHEBI:32599"]
    ing8_Magnesium_sulfate -.-> CHEBI_32599
    ing9_Ammonium_chloride["Ammonium chloride"]
    CultureMech_006991 --> ing9_Ammonium_chloride
    CHEBI_31206["CHEBI:31206"]
    ing9_Ammonium_chloride -.-> CHEBI_31206

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 D-Glucose
C6H12O6 180.156 g/mol
11.0 MILLIMOLAR CHEBI:17634
D-glucose
 Cross-references: KEGG:glc_D
2 L-Proline
C5H9NO2 115.132 g/mol
1.7 MILLIMOLAR CHEBI:17203
L-proline
 Cross-references: KEGG:pro-L
3 Calcium chloride anhydrous
Ca.2Cl 110.984 g/mol
0.1 MILLIMOLAR CHEBI:3312
calcium dichloride
4 Dibasic sodium phosphate
HO4P.2Na 141.958 g/mol
49.0 MILLIMOLAR CHEBI:34683
disodium hydrogenphosphate
5 Leucine
C6H13NO2 131.175 g/mol
0.38 MILLIMOLAR CHEBI:15603
L-leucine
6 Sodium chloride
Cl.Na 58.443 g/mol
8.6 MILLIMOLAR CHEBI:26710
sodium chloride
7 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.0007 MILLIMOLAR CHEBI:49105
thiamine hydrochloride
8 Potassium dihydrogen phosphate
H2O4P.K 136.084 g/mol
22.0 MILLIMOLAR CHEBI:63036
potassium dihydrogen phosphate
9 Magnesium sulfate
Mg.O4S 120.368 g/mol
2.0 MILLIMOLAR CHEBI:32599
magnesium sulfate
10 Ammonium chloride
Cl.H4N 53.492 g/mol
19.0 MILLIMOLAR CHEBI:31206
ammonium chloride

Preparation

  1. Dissolve all ingredients in distilled water to specified concentrations
  2. Adjust pH if specified in original formulation
  3. Sterilize by filtration (0.22 μm) to preserve heat-sensitive components

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T07:21:43.546938Z mediadb-import Imported from MediaDB Source: MediaDB (Institute for Systems Biology), Medium ID: 104, Reference: Mazumdar et al. (2014) PLOS One
2026-03-13T06:19:45.479978Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:006991
2026-03-17T06:30:17.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:03.556683+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "'M9 (Seo" to "m9_seo" for consistent matching
2026-06-05T07:41:12.096122+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-31T06:17:36.574456+00:00 repair_mediadb_names.py REPAIRED_MEDIADB_TRUNCATED_NAME Restored the medium's own name in media_term.term.label, original_name. The MediaDB SQL parser truncated every value containing a parenthesis at that parenthesis; recovered from media_database.07Oct2015.sql.