Overview
- External ontology
- MEDIADB:118 (source link unavailable) — histidine limiting minimal media (Hassan et al)
- Applications
-
- Cultivation of genome-sequenced organisms
- Metabolic modeling
- Systems biology research
- Notes
- Source: MediaDB (Chemically-defined media for genome-sequenced organisms) Reference: https://mediadb.systemsbiology.net/
Composition
flowchart LR
CultureMech_007005["histidine_limiting_minimal_media_hassan_et_al"]
ing0_D_Glucose["D-Glucose"]
CultureMech_007005 --> ing0_D_Glucose
CHEBI_17634["CHEBI:17634"]
ing0_D_Glucose -.-> CHEBI_17634
ing1_Citrate["Citrate"]
CultureMech_007005 --> ing1_Citrate
CHEBI_16947["CHEBI:16947"]
ing1_Citrate -.-> CHEBI_16947
ing2_Thiamine["Thiamine"]
CultureMech_007005 --> ing2_Thiamine
CHEBI_18385["CHEBI:18385"]
ing2_Thiamine -.-> CHEBI_18385
ing3_Histidine["Histidine"]
CultureMech_007005 --> ing3_Histidine
CHEBI_27570["CHEBI:27570"]
ing3_Histidine -.-> CHEBI_27570
ing4_Potassium_dibasic_phosphate["Potassium dibasic phosphate"]
CultureMech_007005 --> ing4_Potassium_dibasic_phosphate
CHEBI_131527["CHEBI:131527"]
ing4_Potassium_dibasic_phosphate -.-> CHEBI_131527
ing5_Magnesium_sulfate["Magnesium sulfate"]
CultureMech_007005 --> ing5_Magnesium_sulfate
CHEBI_32599["CHEBI:32599"]
ing5_Magnesium_sulfate -.-> CHEBI_32599
ing6_Sodium_ammonium_phosphate["Sodium ammonium phosphate"]
CultureMech_007005 --> ing6_Sodium_ammonium_phosphate
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
D-Glucose
C6H12O6 180.156 g/mol
|
27.7531 MILLIMOLAR |
CHEBI:17634
D-glucose
|
Cross-references: KEGG:glc_D |
| 2 |
Citrate
C6H5O7 189.099 g/mol
|
9.51728 MILLIMOLAR |
CHEBI:16947
citrate(3-)
|
Cross-references: KEGG:cit |
| 3 |
Thiamine
C12H17N4OS 265.362 g/mol
|
0.113053 MILLIMOLAR |
CHEBI:18385
thiamine(1+)
|
Cross-references: KEGG:thm |
| 4 |
Histidine
C6H9N3O2 155.157 g/mol
|
0.0643087 MILLIMOLAR |
CHEBI:27570
histidine
|
|
| 5 |
Potassium dibasic phosphate
HO4P.2K 174.174 g/mol
|
57.405 MILLIMOLAR |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
Magnesium sulfate
Mg.O4S 120.368 g/mol
|
0.811458 MILLIMOLAR |
CHEBI:32599
magnesium sulfate
|
|
| 7 | Sodium ammonium phosphate | 16.7394 MILLIMOLAR |
Preparation
- Dissolve all ingredients in distilled water to specified concentrations
- Adjust pH if specified in original formulation
- Sterilize by filtration (0.22 μm) to preserve heat-sensitive components
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T07:21:43.656260Z | mediadb-import | Imported from MediaDB | Source: MediaDB (Institute for Systems Biology), Medium ID: 118, Reference: Mazumdar et al. (2014) PLOS One |
| 2026-03-13T06:19:45.547025Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007005 |
| 2026-03-17T06:30:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:03.633164+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "'histidine limiting minimal media (Hassan et al" to "histidine_limiting_minimal_media_hassan_et_al" for consistent matching |
| 2026-06-05T07:41:08.561074+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-31T06:17:34.058739+00:00 | repair_mediadb_names.py | REPAIRED_MEDIADB_TRUNCATED_NAME | Restored the medium's own name in media_term.term.label, original_name. The MediaDB SQL parser truncated every value containing a parenthesis at that parenthesis; recovered from media_database.07Oct2015.sql. |